20?10 9/L) was observed (mean 41 days, range 12 to 118 days). There w as the association between the number of CD34 +CD44 + cells infusion and time to neutrophic recovery (?= -0.657 , P

China ; epidemiology ; Hong Kong ; epidemiology ; Humans ; Microscopy, Electron ; SARS Virus ; genetics ; growth & development ; ultrastructure ; Severe Acute Respiratory Syndrome ; epidemiology ; prevention & control ; virology

Objective To compare the current trcmment of intertrochanteric fracture fixation commonly used in the two systems:proximal femoral nail(PFN-A)and the dynamic hip screw(DHS)of clinical efficacy and evaluate the treatment of the two intertroehanteric fracture of the gifted bad.Methods The clinical use on closed reduction and traction,respectively PFN-A with DHS and the treatment of intertrochanteric fracture patients with 106 cases and 115 cases,compared with its ease of operation,and the effect of surgery,postoperative complications.Results The clinical material demonstrated that in after PFN-A treatment thighbone thick pmsperom bone fracture technique,technique,the complication is short in DHS.Conclusion PFN-A well-designed,with simple anti-rotation indeed,surgical trauma,less bleeding,fewer complications,etc.,is the treatment of intertrochanteric fracture fixation one of the ideal.

Objective To observe the arrangement and distribution of the fascicular groups in the ulnar nerve trunk to the intrinsic muscles so as to provide anatomical evidence for selective repair in the clinic.Methods 12 cadaveric upper extremities recently embalmed with formalin were obtained. The ulnar nerve and its branches were exposed. Microanatomy and longitudinal contradissection of each functional fascicular group were performed by tracing their terminal branches, and the arrangement and distribution were observed and recorded. The fascicular groups to the intrinsic muscles of hand in the nerve trunk were observed to see how they crossed and mixed with other fascicular groups. The diameter of the nerve trunk and the fascicles to the intrinsic muscles were measured, and the area and the distribution range of the fascicles calculated. Results The shape and the diameter of the ulnar nerve trunk varied at different positions. The fibers of functional fascicular groups had the definite differentiation at distal while interfused and diffused at proximal. The fascicular groups to the intrinsic muscles of hand were the largest and their fibers relatively concentrated (the distribution range occupied 40% ～ 80% of the ulnar nerve), and had the definite localization at the forearm and distal to the 3/8 segment of upper arm. Conclusion Since the fascicles to the intrinsic muscles of the hand are the most important in the ulnar nerve, selective repair by using epineural and perineural techniques at the forearm and upper arm will reduce the loss of nerve fibers and will contribute to the functional recovery.

Objective To observe the level of the nitric oxide (NO) and endothelin-1 (ET-1) in the exhaled breath condensate(EBC)and serum of the patients with ALI /ARDS, and investigate its clinical significance. Methods The study group included 52 mechanical ventilation patients with ALI/ARDS in ICU , which were divided into the survival and death group, while 30 healthy volunteers were recruited as healthy control. EBC samples of the healthy control and the study group on the 1st day and 5st day were collected by EcoScreen condenser with the synchronous collection of the venous blood. The concentrations of NO and ET-1 in the EBC and serum were measured by EIA. Results The levels of NO and ET-1 in EBC and serum of the patients with ALI /ARDS were all significantly higher than those of the healthy control. After treatment , the levels of NO and ET-1 in EBC and serum of the patients all decreased significantly compared with before treatment. After treatment , The levels of NO in EBC and serum of the survival group were significantly lower than those of the death group. After treatment , the levels of ET-1 in serum of the survival group was significantly lower than that of the death group. Conclusions Detecting the levels of NO and ET-1 in the EBC and serum can reflect oxidative stress , inflammatory reaction and endothelial injury in lung of patients with ALI/ARDS.

Objective To discuss the features of MRI and molybdenum target X-ray in mammary primary non-Hodgkin's lymphoma.Methods 3 cases of mammary primary non-Hodgkin's lymphoma were studied.The features of MRI and molybdenum target X-ray werecollected and analyzed(all 3 cases had molybdenum target X-ray in MLO site and CC site,one had MRI image).Results All 3 caseslesions were in single side,one case had two lesions in one side.The diameter of all the lesions were more than 3cm.The lesion presented as iso-or slight hyperdensity shadow,and related to breast tissue and density was hemogeneous.The tumor was irregular but no bristle andcalcification.There was no skin and nipple involved in superficial of mammary gland.In T_1WI showed even low signal.In T_2WI showedeven little high or equal signal.Intensity was hemogeneous and obvious.The curve of time-signal intensity was platform pattern.Conclusion The features of MRI and molybdenum target X-ray in mammary primary non-Hodgkin's lymphoma are characteristic and helpful for deciding correct therapy plan.

Objective To reconstruct on the basis of histological study the computerized 3D ima ges of the fascicular groups of ulnar nerve,which can be used to guide the microsurg ical repair of injuries of brachial plexus and ulnar nerve and to provide morpholog ical evidence for the functional rec overy of intrinsic muscles of hand after root avulsion of brachial plexus.Methods The successive AchE-staining histo logical cross-sections were made.T he photos of the sections were transformed into digital information and sto red in the computer.Different functional fas-cicular groups were distinguished a nd marked on each section.The topographic database was analyzed by image pro-cessing software,with the support o f OpenGL three dimensional graphic l ibrary.3D images of the fascicular g roups of ulnar nerve were reconstructed usin g the contour-based surface constru ction method in the program written w ith VC language.Results The 3D images of the individual external surface of the reconstructive fa scicular groups were displayed and the human-computer in teraction was realized by performin g the arbitrary rotation and the rand om cross-section.By selecting the int erested object,the spatial structu re of any fascicular group could be di splayed in the same space simultaneously.Compare d with the result of microanatomy,th e 3D shape could truly reflect the str ucture,adjacent relationship and arrangem ent of fascicular groups in the ulnar nerve trunk.Conclusions Since the re-constructive 3D images can truly reflect the arrangement and distribution of the functional fascicular grou ps,they will have positive effects on understanding the internal structure of ulnar nerve,designing operative procedur e and adjusting operative method for surgeons.[

Objective: To establish a system and method for screening plant extract against mitomycin C-induced genotoxic damage. Methods: Salmonella typhimurium TA1535/pSK1002 and acute toxicity experiment of mice were used, and the effects of the extracts from ten plants, Chrysanthemis Flos, Allii Bulbus, Zingiberis Rhizoma Recens, Ginkgo Folium, Ginseng Radix et Rhizoma, Vitis Viniferae Semen, Gemmae Camelliae Sinensis Folium, Ganoderma, Acanthopanacis Senticosi Radix et Rhizoma seu Caulis, and Sojae Semen, and the extract combinations on mitomycin C-induced genotoxic damage were observed by SOS/umu test. Results: Significantly protective effects of five extracts, including the extracts from Allii Bulbus, Vitis Vindferae Semen, Gemmae Camelliae Sinensis Folium, Acanthopanacis Senticosi Radix et Rhizoma seu Caulis, and Sojae Semen against mitomycin C-induced genotoxicity were observed. Acanthopanacis Senticosi Radix et Rhizoma seu Caulis extract (1.5 g/L) could inhibit the mitomycin C-induced genotoxicity (67.12%). Combinations of any two extracts showed higher antimutagenic capacity than any single one. Among all the combinations, Acanthopanacis Senticosi Radix et Rhizoma seu Caulis-Gemmae Camelliae Sinensis Folium and Acanthopanacis Senticosi Radix et Rhizoma seu Caulis-Vitis Viniferae Semen showed the highest activity, and the inhibition rate of the former against the mitomycin C-induced genotoxicity was 83.2%. In vivo tests showed that Acanthopanacis Senticosi Radix et Rhizoma seu Caulis- Gemmae Camelliae Sinensis Folium could significantly decrease the micronucleus rate and sperm abnormality rate of mice induced by mitomycin C and also increase the thymus indexes. Conclusion: Based on the results, it is clearly proved that the SOS/umu is not only a useful and convenient way to evaluate the antimutagenic ability of plant extracts, but also could be used as a kind of rapid screening model for cytoprotector with high throughput screening of candidate extracts or compounds.

Female ; Genetic Predisposition to Disease ; Hepatitis B ; genetics ; Hepatitis B virus ; Humans ; Polymorphism, Genetic ; Pregnancy ; Pregnancy Complications, Infectious ; virology ; Promoter Regions, Genetic ; genetics ; Tumor Necrosis Factor-alpha ; genetics

Objective:To observe the expression of BTLA on T cells during activation and further analyze its inhibitory effects on T cell activation in different phases.Methods: T cells from PBMC were enriched by negative selection using magnetic beads.Expression of BTLA,CTLA-4 and PD-1 on freshly isolated human T cells and kinetics expression of BTLA,CILA-4 and PD-1 on CD3 mAb stimulated T cells were examined by flow cytometry.T cells were stimulated by anti-CD3 mAb combined with anti-CD28 mAb in the presence of anti-BTLA mAb 8H9,then T cell proliferation was tested by MTT assay in the different culture time.Immature DCs were generated from monocytes cultured in the medium containing GM-CSF and IL-4, and further driven to maturation by anti-CD40 mAb.Expression of HVEM on DCs was measured by flow cytometry.T cells were co-cultured with DCs in the presence of soluble 8H9 or anti-HVEM antibody to block HVEM-BTLA interaction,T cell proliferation was measured by MTT assay.Results:Freshly isolated T cells exhibited high levels of BTLA expression, but not CTLA-4 and PD-1.After T cell activation, BTLA expression decreased on first 2 days, with rapidly increasing to high levels.Unlike BTLA, expression of CTLA-4 and PD-1 was gradually increased during T cell activation.8H9 significantly inhibited the proliferation of T cell stimulated by CD3 mAb and CD28 mAb.8H9 could still exhibit inhibitory effect on T cell proliferation after 24 h or 48 h of preactivation by CD3 mAb plus CD28 mAb stimulation.HVEM was highly expressed on immature DCs, and down-regulated on mature DCs.Blockade of BTLA by soluble 8H9 or anti-HVEM antibody enhanced DC-mediated T cell proliferation within 48 h.Conclusion: BTLA signal enhances the threshold of T cell activation and plays importantly negative regulatory role in the initiation and early phase of T cell activation.