Objectives To observe the changes of apoptosis-related proteins during the development of sarcopenia and to explore the effect and mechanism of long-term aerobic exercises and heat shock protein 70 (HSP70) on those changes for the natural aging C57BL/6 mice.Methods After 1 week of acclimatization,50 27-week-old male C57BL/6 mice were randomly divided into a young sedentary control (YS) group,an old sedentary control (OS) group and an old exercises (OE) group.After the gripstrength was measured the mice from group YS were sacrificed at the age of 28 weeks.The mice from group OE exercised on a fiat treadmill at 12rn/minute for 50 min 3 or 4 times a week for 30 weeks with the intensity of 75％ VO2max.At the age of 60 weeks,the mice of group OS and OE were killed after their grip-strength was measured.The gastrocnemius muscle of each group were isolated and weighted,and the sarcopenia index (SI) was measured.Then the total protein of the gastrocnemius muscle was extracted.HSP70 and cleaved caspase-9 expression were examined.The binding rate of HSP70 and Apaf-1 was measured using Co-Immunoprecipitation.TUNEL staining was performed to detect the apoptosis of the gastrocnemius muscle.Results (1) Compared with group YS,SI of group OS decreased significantly by 23.09％,but significant increase was observed in group OE compared to group OS by 30.48％.(2) The grip strength per gram of body weight of group OS decreased significantly by 42.74％ compared to group YS,while that of group OE increased significantly by 21.51％ compared to group OS.(3) The apoptosis index increases by 475.28％ with age.However,the index decreased by 46.41％ in group OE over group OS.(4) Western blotting showed that the expression of cleaved caspase-9 increased significantly by 45.4％ in group OS compared to group YS,while that of group OE decreased significantly by 33.4％ compared to group OS.(5) The binding rate of HSP 70 with Apaf-1 of group OS decreased significantly by 67.11％ compared to group YS,while that of group OE increased significanlty by 306.31％ compared to group OS.Conclusions (1) Gastrocnemius atrophy occurring to mice of 60 weeks can be significanlty delayed through 30-week exercises training.(2) The increasing apoptosis may be involved in the development of age-related sarcopenia and 30-week exercises can significantly attenuate such apoptosis in rodent skeletal muscle.(3) Aerobic exercises increases HSP70/Apaf-1 interaction,which might play an important role in reducing age-related cellular damage and cell death in the skeletal muscle of rats.

Objective: To analyze the relationship among cardiopulmonary endurance, body composition, flexibility, muscle strength and endurance with functional movement performance of male college students, and to explore the factors associated with the performance of functional movements. Methods: A total of 112 male college students were selected, muscle power and flexibility test was based on the national student physical health standard, founctional movement screen created by Cook was used to test founctional movement performance. Results: Male college students have poor flexibility, stability and control of functional movements, and high probability of compensatory or asymmetric movements; In-line lunge, trunk stability push-up were positively associated with cardiorespiratory fitness(r=0.17, 0.17, P<0.05); hurdle step, shoulder mobility, trunk stability push-up and rotary were negative associated with body composition(r=-0.17, -0.13, -0.25, -0.22,P<0.05); shoulder mobility, trunk stability push-up and rotary were positively associated with score of standing long jump, trunk stability push-up was positively associated with endurance of deltoid and trapetus as well as endurance of pectoralis major and latissimus dorsi; shoulder mobility was positively associated with score of sit and reach(r=0.17, P<0.01). The independent risk factors of functional performance of male college students, hurdle step(β=-0.02), trunk stability push-up (β=-0.12) and rotary were body fat rate(β=-0.03); the protective factors trunk stability push-up were body fat rate(β=-0.04), VO 2max (β=0.04) and endurance of pectoralis major and latissimus dorsi(β=0.03). Conclusion The functional performance of male college students was not optimistic, which could be improved by increasing health-related physical fitness.