Objective To observe the influence of bacterial lysates (broncho-vaxom) combined with montelukast sodium on serum immunoglobulin, interferon (IFN)-γ and interleukin (IL)-4 in asthma and the clinical efficacy. Methods Thirty-one patients with asthma in acute period of onset in children (study group) received broncho-vaxom combined with montelukast sodium for 3 months on the basis of conventional treatment. The changes of serum IgG, IgA, IgM, IFN-γ and IL-4 levels before and after treatment were observed and compared with 31 healthy children (control group), the score changes of day and night symptom were also observed before and after treatment. Results IgG and IgA levels were abnormal significantly in study group before treatment (P < 0.05 ), they were significantly increased after treatment, but IgG was still significantly lower than that in control group (P < 0.05 ). IFN-γ and IL-4 were significantly abnormal in study group before treatment (P < 0.05), IFN-γ was significantly increased after treatment (P<0.05), while IL-4 was significantly decreased (P<0.05); after 3 months of treatment, the score of day and night symptom in study group were (0.4±0.1 ) and (0.7±0.2) scores respectively, which both decreased significantly than those before treatment [(2.6±0.3 ) and (3.4±0.7) scores respectively],the difference were statistically significant(P < 0.05 ). Conclusion Children with asthma have the existence of decreased immune function, application of broncho-vaxom combined with montelukast sodium can effectively control its symptoms and improve immune status.

Objective To discuss the validity and the feasibility of modified specific immunotherapy of hypoglossis allergen in the treatment of children allergic asthma. Methods 100 children with allergic asthma were selected from October,2007 to April ,2008 in our hospital and divided into the observation group and the control group. The control group adopted routine method, the observation group made modifi-cation based upon routine nursing, placing emphasis on intervention of cognition and behavior of children and their parents, improvement of treatment compliance, whole- process, dynamic and continuous observa-tion of the treatment process, making individualized health education plan. The treatment compliance, total score of asthma control, and pulmonary function examination of impulse oscillation(IOS) were compared be-tween the two groups. Results The observation group was superior to the control group in treatment com-pliance, pulmonary function examination and the control results of asthma. Conclusions Specific im-munotherapy of hypoglossis allergen combined with modified nursing method can increase treatment com-pliance of children and lead to better results.

AIM: To investigate the immune stimulation capacity of B7-H1 blockade on immature dendritic cells (DCs) in vitro. METHODS: The human monocyte-derived dendritic cells were induced in the presence of cytokine GM-CSF and IL-4. The expression of B7-H1 was detected by FCM. On blockade of B7-H1, the maturation and endocytic activity, T cells stimulatory proliferation capacity, IL-12 production, T cell differentiation effect of DCs were detected by FCM, MTT assay, ELISA and ELISPOT, respectively. RESULTS: The expression of B7-H1 was increased with the induction of DCs. On day 7, the positive expression was 54.12%, and the TNF-? induced mature DCs had the positive expression rate of 83.64%. The blockade of B7-H1 on immature DCs had sharply increased their T cells stimulatory proliferation capacity and IL-12 production, and efficiently induced the development of Th1/Tc1 cells, but had no effect on their maturation and endocytic activity. CONCLUSION: The blockade of B7-H1 on immature DCs increases its immune stimulation activity. It is valuable to investigate the antitumor immune responses of DCs vaccine with B7-H1 blockade.