Objective To discuss the therapentic efficacy of hyperbaric oxygen combined with PC program in newly diagnosed epithelial ovarian cancer patients. Methods Fifty-eight patients with epithelial ovarian cancer were divided into two groups by random digits table: HBO group(30 cases) and PC group(28cases). HBO group were exposed to hyperbaric oxygen of 2 standard atmospheric pressure 60 min, then given chemotherapy 25-30 min after extravehicular: cyclophosphamide 1000 mg/m2 + cisplatin 75 mg/m2.PC group with the same regimen without hyperbaric oxygen therapy were analyzed. The two groups were compared in the efficacy and 3-year survival rate, progression-free survival and adverse reactions. Results The total effective rate, not controlled rate, recurrence rate,recurrence time, 3-year survival rate in HBO group [83.3%(25/30),6.7%(2/30),33.3%(10/30), (21.0 ± 0.8) months,43.3%(13/30)] were better than those in PC group [67.9% (19/28), 17.9% (5/28), 46.4% (13/28), (18.0 ± 0.6) months, 17.9% (5/28)] (P ＜0.05), progression-free survival and overall survival time in HBO group were longer than those in PC group (P ＜0.05) and adverse reactions rate in HBO group was lower than that in PC group (P ＜0.05).Conclusions The hyperbaric oxygen combined with PC programs are better than PC programs in advanced epithelial ovarian cancer chemotherapy response rate, progression-free survival time and 3-year survival rates in ovarian cancer adjuvant chemotherapy. HBO can significantly reduce the PC's hematological toxicity and toxicity of the digestive system.

Objective To observe the clinical curative effect of patients with ovulation obstructive infertility by combined treatmentofstimulating ovulation particles and clmiphene citrateclinical. Methods 171 patients were randomly divided into Traditional Chinese medicine (TCM) group (42 cases), clmiphene citrateclinical (CC) group (53 cases) andcombined treatment of TCM and CC group (76 cases). Ultrasound examinations were used to monitor the development of endometrium, follicular development and value of Plusatility Index (PI). The levels of E2,LH and P were measured before and after medication. And pregnancy of patients was followed up. Results Ovulation rates of combined treatment of TCM and CC group and the CC group were both higher than TCM group (P<0.05). Pregnancy rate of combined treatment of TCM and CC group was higher than TCM group and CC group (P<0.05). Endometrial thickness and type A lining rate of combined treatment of TCM and CC groupandTCM group were both higher than CC group (P < 0.05). The values of PI of combined treatment of TCM and CC groupand TCMgroup were both lower than CC group on HCG day after treatment (P<0.05). E2 values ofpost-treatment of three groups were all significantly higher than that before treatment.Difference in values of E2 and LH between pretherapy and post-treatment of combined treatment of TCM and CCwere obviously higher than that of TCM group (P<0.05).Difference in values of P between pretherapy and post-treatment of tcombined treatment of TCM and CC and TCM group were higher than CC group on the seventh day after ovulation (P < 0.05). Conclusions Combination of traditional Chinese medicine and CCcouldnot only promote follicle growth , but also improve anti-estrogen role of CC , endometrial blood flow and endocrine regulation , enhance ovulation rate and decrease the rate of abortion. Ovulation obstructive infertility treatment was better than that of CC and TCM treatment.

Objective To explore the optimal experiment conditions of CCK-8 and MTS for cell proliferation assays in human amniotic epithelial cells and to evaluate the cytotoxicity of these reagents. Methods Human amniotic epithelial cells (hAECs) in logarithm growth stages were prepared in different cell concentrations with DMEM/F12 and 10% FBS. The sensitivity and optimal wavelengths was determined based on the optical density (OD) measured at 450 nm and 492 nm. The optimal time was determined under the conditions of the same cell concentration and defined OD values. HAECs were treated with DMSO, CCK-8 and MTS for 1 h, 2 h, 3 h, and 4 h, respectively. 24 h later, cytotoxicity of the CCK-8 and MTS was evaluated by determination of cell proliferation and Trypan Blue staining. Results The optimal detection wavelength was 450 nm for CCK-8, and 492 nm for MTS. The sensitivity of CCK-8 was slightly lower then that of MTS. The optimal time for incubation hAECs with CCK-8 was 4 h within 1~4 h. The inhibitory on cell proliferation and cytotoxicity of CCK-8 were weaker then those of MTS. Conclusion CCK-8 is a convenient reagent with low cytotoxicity for detection of the proliferation of hAECs.