[Objective]To discuss the treatment to post-apoplexy depression with acupuncture of strengthening brain and regulating mind.[Method] Select 60 cases;randomly divide them into acupuncture group and western medicine group with 30 cases in each.Both accept basic therapy,the acupuncture group is added with acupuncture of strengthening brain and regulating mind,the other with fluxetine;for successive 4 weeks make scores to HRMD mete table,nerve function lack degree and daily life Barthel index.[Result] Both scores had changes after treatment,however the acupuncture group was better than another group,with marked cure effect,meanwhile with quantity-efficacy relationship.[Conclusion] Acupuncture of strengthening brain and regulating mind can relieve depression,promote nerve function recovery and improve life quality.

Aim To investigate the protective effect of different concentrations of isoflurane preconditioning in isolated rat hearts and the relationship between this effect with changes of endogenous antioxidant enzymes.Methods The isolated SD rat hearts perfused on Langendorff apparatus were randomly divided into 6 groups(n=14):control group(CON);1.44 MAC isoflurane control group(ISO);ischemia-reperfusion group(I/R);3 isoflurane preconditioning groups(I1,I2,I3).All hearts were made globally ischemic for 30 min followed by 60 min reperfusion except CON and ISO.The hearts of ISO and I1,I2,I3 were perfused with K-H solution saturated with 0.72 MAC or 1.08 MAC or 1.44 MAC isoflurane respectively for 20 min followed by 5 min washout after equilibrium.The cardiac function variables were measured after equilibrium,immediately before ischemia,at the end of 30 min and 60 min reperfusion.The infarct size and activities of the endogenous antioxidant enzymes were measured at the end of reperfusion.And the activities of enzymes in I2 group were measured after equilibrium,immediately before ischemia,immediately after ischemia and at the end of 30 min reperfusion.Results Ischemia/reperfusion significantly increased LVEDP and decreased LVDP,dp/dtmax,dp/dtmin and HR(P

0.05).Inside experimental area,pain scores were decreased in both intradermal 0.25% lidocaine and saline injection group(P

Aim To explore the effect of propofol preconditioning on cardiocyte apoptosis and cytochrome C release from mitochondria during hypoxia/reoxygenation in isolated rat hearts.Methods Fifty male Sprague-Dawley rats weighing 250～300 g were randomly divided into 5 groups(n=10 each):control group(C);Dimethyl sulfoxide(DMSO)preconditioning group(D);3 propofol preconditioning groups with 25 ?mol?L-1(P1)、50 ?mol?L-1(P2)、100 ?mol?L-1(P3)propofol respectively.The isolated rat hearts were retrogradely perfused via aorta with K-H solution on Langendorff apparatus.The isolated hearts were made hypoxia for 30 minutes followed by 60 minutes reoxygenation in each group.The D,P1,P2,P3 groups were preconditioned by perfusing 10 min K-H solution containing 20 ?mol?L-1 DMSO and 25,50,100 ?mol?L-1 propofol respectively and then followed by 5 min K-H solution reperfusion before hypoxia.The preconditioning procedure was repeated twice.The cardiac functional variables were recorded after equilibration(baseline values),immediately before hypoxia,at the end of 30 min and 60 min reoxygenation.Apoptotic myocardial cells were detected by terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling(TUNEL)and the level of cytochrome C expression in myocardial cytosol and mitochondria was measured by Western blot at the end of reoxygenation.Results At the end of 30 min and 60 min reoxygenation,LVEDP was significantly lower and LVDP was significantly higher in P1,P2,P3 groups than in D group(P

Objective To explore the feasibility of the ultrasonic atomization surface anesthesia with lido-caine for awake fiberoptic endotracheal intubation in patientsˊautonomous position -display before general anesthesia and to evaluate its advantages.Methods 68 adult patients who needed prone position for elective surgery under general anesthesia were selected and randomly divided into two groups,the control group and the treatment group,each group in 34 cases.In control group patients were received surface anesthesia of cricothyroid membrane puncture.In treatment group,nebulized 2% lidocaine with ultrasonic nebulizer was used for topical anesthesia.Patients lied in the prone position according to their own comfort with the guide of the medical staff in the waking state after an awake fiberoptic endotracheal intubation.The statistics of mean arterial pressure (MAP)and heart rate (HR)were recorded respectively in the basal state(T0),in the time instantly after intubations(T1 ),in the 3 minute after intubations(T2 ) and in the time instantly after the body turning(T3 ).Choking cough response were recorded during endotracheal intubation.Patients were asked the efficacy of surface anesthesia and the tolerance for awake intubation after operation.Results Patients in both two groups completed the whole process smoothly.MAP and HR had no signifi-cant differences between the two groups in the same time point (all P >0.05).There were no statistical significance between the two groups in choking cough response,the time of surface anesthesia and intubation,neither (all P >0.05).Conclusion The surface anesthesia with lidocaine by continuous ultrasonic atomizing inhalation is a good and simple method deserving generalization with plenty merits and is practicable for patients to display position autonomously. This method have the advantages of small operation,it will and can replace cricothyroid membrane puncture.

Objective To perform a meta-analysis on the association of of genotype B/C and HBV DNA conversion of negative or HBeAg clearance to lamivudine therapy.Methods Several databases (PubMed, Web of Science, Science Direct, and CNKI Database) were searched from 1966 to 2012 for all publications on the association between genotype B/C and response to LAM therapy.The articles were selected according to the protocol previously designed.Meta analysis using Revman 5 software.Results Finally, 19 RCTs were retrieved involving 3148 patients for the subsequent meta-analysis. Among them, 10 articles (n=1860) look at HBV DNA and 9 (n=1288) at HbeAg clearance.For HBV DNA conversion of negative, the overall RR (95% CI) associated with genotype B/C was 1.07(0.98-1.17). Of the nine analyzed trials, HBeAg clearance was observed in genotype B group as compared with that genotype C group, the overall RR (95% CI) was 1.27 (0.94-1.71).Conclusion Meta-analysis indicates that genotype B/C is not associated with response to LAM therapy.Further mechanism researches are required to clarify.Large-scale population studies in multicountries are also necessary to evaluate the influence of HBV genotypes in hepatitis B progression and antiviral treatment.

Objective To investigate the role of gamma-aminobutyric acid transporter-1 (GAT-1) in the spinal cord in a rat model of bone cancer pain.Methods Eighty female SD rats weighing 150-180 g were randomly divided into 5 groups (n =16 each): sham operation group(group Ⅰ ),bone cancer pain group(group Ⅲ ),sham operation+ NO-711 group(group Ⅲ ),Ⅳ group BCP + NO-711 group(group Ⅳ ) and BCP + vehicle group (group Ⅴ ).Bone cancer pain was induced by inoculating Walker-256 mammary gland carcinoma cells into medullary cavity of tibia.NO-711 (20 μg,10 μl) was administered intrathecally once a day for 3 consecutive days from the 14th day after operation.Mechanical withdrawl threshold (MWT) of mechanical stimulus was determined the day before operation and at days 3,5,7,10,14 and 16 after operation.The animals were sacrificed on the 16th day after operation,and then the spinal cords were removed for determination of the expression of GAT-1 and double immunostaining of GAT-1 and glial fibrillary acidic protein (GFAP,astrocyte marker).Results MWT were significantly decreased in groups Ⅱ,Ⅳ and Ⅴ as compared with groups.Ⅰ and Ⅲ.The expression of GAT-1 significantly up-regulated in groups Ⅱ,Ⅴ as compared with groups Ⅰ and Ⅲ.NO-711 significantly increased MWT,while decreased the expression of GAT-1 in group Ⅳ compared with groups Ⅱ and Ⅴ.The expression of GAT-1 up-regulation appeared colocalizes with in astrocytes activation in spinal dorsal horn.Conclusion The up-regulation of expression of GAT-1 in spinal cord is involued in the development and maintenance of bone cancer pain,which may be related to the astrocytes activation.

Objective To research the effect of postoperative intervention on the quality of life of cervical cancer patients, in order to provide the basis for improve the quality of life of patients after operation. Methods 86 cases of cervical cancer were divided into control group and intervention group by simple random grouping method, each group contained 43 cases. The postoperative interventions were used in the intervention group after 3 months, including psychological assessment, individual education, pelvic floor rehabilitation therapy, open patient service hotline and regular follow-up. Before and after 6 months of surgery, the quality of life of cervical cancer patients were investigated. Results The index score of social relations [(48.84±4.63) scores vs. (40.72±3.74) scores], psychological field [(73.72±2.84) scores vs. (64.07±4.06) scores], environmental field [(64.07±4.08) scores vs. (55.70±4.86) scores], physiological index [(44.77±4.22) scores vs. (36.53±4.29) scores], independence [(64.81 ±5.85) scores vs. (53.95±5.91)], quality of life [(88.30±5.75) scores vs. (76.65±5.21) scores] in cervical cancer patients were lower than those in the control group (all P<0.05). Multiple stepwise regression analysis showed that the understanding degree of cervical cancer, radiotherapy and chemical therapy after surgery, postoperative complications, caregivers care in place and psychological intervention had correlation with the quality of life of patients in intervention group (all P<0.05), while retain ovarian and education degree had no correlation with the quality of life of patients after operation (both P>0.05). Conclusions The quality of life of cervical cancer patients after surgery is poor, and it is affected by many factors. The targeted interventions should be used in clinical works, which can significantly improve the quality of life of patients.

Objective To research the trichomonacidal effect of secnidazole benzoate in vitro.Methods Trichomonas vaginalis was cultured in liver extract medium in 96-well microplate.The culture suspension of Trichomonas vaginalis was divided into four groups:secnidazole benzoate, secnidazole, metronidazole and control, with medium as blank control.MTT colorimetric assay was applied to determine the inhibitory effect of secnidazole benzoate on the proliferation of Trichomonas vaginalis.The culture suspension was transfered into test tubes and divided into same groups to observe inhibitory effect by the classical microscopic counting method.Results After 24 h incubation, the proliferation of the parasites was concentration-dependent by secnidazole benzoate(t=9.02, P