The latest study shows that the expression abnormalities of programmed death 1/programmed death ligand 1 (PD-1/PD-L1) signaling pathway in a variety of solid tumor tissues and cells are related to disease progression and poor prognosis, and directly involve in tumor immune escape, becoming the recent focus. With the further research, PD-1/PD-L1 signaling pathway in blood diseases, for example, in the development of lymphoma, is catching more attention gradually. The signaling pathway not only is associated significantly with malignancy, progression, recurrence of lymphoma and prognosis of patients, but probably becomes a new target for cancer immunotherapy. This paper summarizes the expression and research status of the biological characteristics and mechanism of PD-1/PD-L1 signaling pathway in lymphoma, in order to provide a new way for lymphoma treatment.

The P2X7 receptor (P2X7 R)is a nonselective cation channel activated by extracellular ATP,and it may trigger the se-cretion of several proinflammatory substances,such as IL-1β, IL-18,TNF-α,and nitric oxide.Several preclinical studies have demonstrated that this receptor participates in inflammation and pain mechanisms,and compounds that modulate the function of this receptor show potential as new anti-inflammatory medicines. Therefore,P2X7 receptors play particularly important physiologi-cal roles and have potential clinical application in inflammation and pain,which proves it a therapeutic target for pain manage-ment.

Aim To investigate the protective effect of different concentrations of isoflurane preconditioning in isolated rat hearts and the relationship between this effect with changes of endogenous antioxidant enzymes.Methods The isolated SD rat hearts perfused on Langendorff apparatus were randomly divided into 6 groups(n=14):control group(CON);1.44 MAC isoflurane control group(ISO);ischemia-reperfusion group(I/R);3 isoflurane preconditioning groups(I1,I2,I3).All hearts were made globally ischemic for 30 min followed by 60 min reperfusion except CON and ISO.The hearts of ISO and I1,I2,I3 were perfused with K-H solution saturated with 0.72 MAC or 1.08 MAC or 1.44 MAC isoflurane respectively for 20 min followed by 5 min washout after equilibrium.The cardiac function variables were measured after equilibrium,immediately before ischemia,at the end of 30 min and 60 min reperfusion.The infarct size and activities of the endogenous antioxidant enzymes were measured at the end of reperfusion.And the activities of enzymes in I2 group were measured after equilibrium,immediately before ischemia,immediately after ischemia and at the end of 30 min reperfusion.Results Ischemia/reperfusion significantly increased LVEDP and decreased LVDP,dp/dtmax,dp/dtmin and HR(P

Objective struclured clinical examination (OSCE) is a kind of examination which could objectively evaluate the students′clinical skills.Nowadays,OSCE are wildly applied in medical educational field throughout the world.Timely discussion and analysis on the problems existed in the implementation process of OSCE is necessary.Measures should be taken to improve the OSCE examination and to meet the requirements of higher clinical practice training level such as increasing clinical skill simulation training hardware investment,optimizing settings and conlents of the examination and the test stations as well as introducing standardized patients (SP) and other measures.

Objective:The stimulate angiogenes effect and mechanism of Shexiangbaoxin Pills on coronary collateral development in the hearts of experimental myocardial infarction rats were studied. Methods:24 myocardial infarction rats were randomly divided into 3 groups, 8 rats in group A were treated with Shexiangbaoxin Pills, 8 rats in group B were treated with Beifuji and heparin, and 8 rats in group C were treated with 0.9% normal saline as control. Infarct area, the contents of VEGF、bFGF and VⅢ factor, and vascular density in the edge of infarct section were measured after 6 weeks treating.Results: Infarct area in group A was obviously less than that in group C, the contents of VEGF、bFGF and VⅢ factor in the edge of infarct section and vascular density in group were significantly increased compared with those in group C. Conclusions: Shexiangbaoxin Pills could stimulate angiogenesis of the collateral branch of coronary artery in ischemic myocardium of rats and showed good protective on myocardial ischemia.

Objective To investigate the values of diffusion tensor MR imaging(DTI)and fiber tractography(FT)in the assessment of cognitive functions in children with periventricular leukomalacia (PVL),to facilitate to understand the mechanism of PVL children's cognitive functions.Methods Forty six children with PVL and 16 age-matched volunters were recruited.Developmental quotient(DQ)of all cases was recorded.According to the DQ scores,patients were divided into 3 groups:mild in 11 patients,moderate in 19,and severe in 16.DTI was performed in all cases and fractional anisotropy(FA)values were be calculated.Non-paired t test was used to compare the FA values of major white matter fibers between patients group and controls group.One-way ANOVA was used to compare the FA values among 4 groups.Results Compared with controls,all 46 patients with PVL showed a significant mean FA reduction in left corticospinal tract(CST)(0.476 ±0.064 vs.0.531 ±0.064),bilateral anterior limb of internal capsula (ICAL)(left 0.357 ±0.050 vs.0.405 ±0.081,right 0.370 ±0.040 vs.0.405 ±0.036),posterior limb of internal capsule(ICPL)(left 0.483 ±0.044 vs.0.546 ±0.091,right 0.485 ±0.046 vs.0.547 ±0.083),arcuate fasciculus(AF)(left 0.367 ± 0.069 vs.0.433 ± 0.097,right 0.363 ± 0.064 vs.0.421 ±0.091),posterior thalamic radiation(PTR)(left 0.390 ± 0.059 vs.0.459 ± 0.067,right 0.382 ± 0.047 vs.0.446 ± 0.064),anterior CG(ACG)(left 0.362 ± 0.056 vs.0.423 ± 0.057,right 0.345 ± 0.056 vs.0.417 ± 0.080),superior longitudinal fasciculus(SLF)(left 0.323 ± 0.050 vs.0.426 ±0.102,right 0.341 ± 0.056 vs.0.416 ± 0.092)and splenium of the corpus callosum(SCC)(0.535 ±0.090 vs.0.606 ±0.060)(t =2.037-3.745,P ＜0.05).The severity of cognitive impairment companied with decrease of the FA values of all fibers.The FA values of bilateral CST,ICAL,ICPL,left AF,bilateral PTR,ACG,SLF and SCC were significantly different among different groups(F =3.021-7.146,P ＜ 0.05).Conclusions The damage widespread of white matter bundles is found in children with PVL DTI and FT can provide details in the assessment of cognitive impairment in children with PVL.

Objective To investigate the characteristics of attention networks impairment in patients with lesion in the cerebellum.Methods The attention network test was used to compare patients with lesion in the cerebellum(n=28)with normal controls(n=3.1)on the efficiency of three anatomically defined attention networks:alerting,orienting,and executive control. Results The orienting network effect was significantlyworse(Z=-2.309,P＜0.05)in patients with lesion in the cerebellum((36.32±30.58) ms)than in normal controls((54.39±22.17)ms).The executive control network in patients((160.05± 83.25)ms)with lesion in the cerebellinn was worse than those of controls((93.42±37.41)ms,Z= -3.500,P＜0.01).The alerting networks effects was higher in patients((35.14±45.59)ms)than in normal controls((28.81±26.09)ms),without significant difference.The average reaction time was longer in patients than in normal controls,but there was no significant difference.The wrong rate of attention network testwas significantly higher(Z=-2.119,P＜0.05)in patients(6.57%±9.84%)than in normal controls(3.38%±5.42%).Conclusion The patients with lesion in the cerebellum may be selectively impaired of the orienting and executive networks,while the alering network is spared.

Background Retinal ichemic-reperfusion injury (RIRI) affects vision and life quality and has no ideal treatment outcome up to now.The mechanism of RIRI is associated with apoptosis.Researches showed that subretinal transplantation of bone mesenchymal stem cells (BMSCs) can relieve RIRI,but its mechanism is unclear.Objective This study was to observe the influence of BMSCs subretinal transplantation on the expressions of apoptosis protein Fas/FasL,caspases-3 after RIRI,and to explore the neuroprotecitve mechanism of BMSCs transplantation to treat RIRI.Methods Bone marrow was isolated and extract form femur and tibia of SD rat by the density gradient centrifugation method and then the BMSCs were collected.BMSCs suspension was prepared using DMEM containing low-glucose with the cell density 1 x 106-2× 106/ml.RIRI models were established by ligation of optical nerve (Daniel M method).Sixty-four SD rats were randomly divided into the normal control group,model control group,BMSCs transplantation group and PBS control group.Twenty-four hours after RIRI,BMSCs suspension with the cells density 5× 104 cells was subretinally injected in the rats of the BMSCs transplantation group,and equal volume of PBS was injected in the same way in the rats of the PBS control group.The expressions of Fas/FasL and caspase-3 were dynamically detected using immunohistochemistry at 6,24,48 and 72 hours after BMSCs transplantation.Results The positive response cells for Hoechst33324 were seen in subretina 72 hours after BMSCs transplantation.A few of positive cells for Fas,FasL,caspase-3 proteins were expressed in the rats of the normal control group at 6,24,48 and 72 hours after BMSCs transplantation.However,the positive cells for Fas,FasL,caspase-3 protein were significantly increased in the model control group and the BMSCs transplantation group compared with the normal control group at various time points after injection (all at P＜0.01),and the number of positive cells was significantly less in the BMSCs group than those of the RIRI group and the BMSCs transplantation group (P＜0.05,P＜0.01).No significant differences were found in the numbers of positive cells of Fas,FasL and caspase-3 proteins at various time points between the model control group and the PBS control group (all at P＞0.05).Conclusions The subretinal transplantation of BMSCs down-regulate the expression of Fas/FasL and caspases-3 proteins in RIR1 rats,and thus alleviate the apoptosis of retinal neural cells.

Objective To explore the status and risk factors of the dyslipidemia among health examination population of Lanzhou for providing the intervention measures .Methods According to the stratified cluster random sampling method ,4 505 health exami-nation individuals were recruited for the study from 5 hospitals in the Lanzhou region through questionnaire ,biochemical analysis . Results Prevalence of dyslipidemia of the population was 45 .79% ,high TG was the main type .The level of serum TC ,TG ,HDL-C and LDL-C were (5 .27 ± 1 .08) ,(1 .74 ± 1 .38) ,(1 .41 ± 0 .43) and (2 .83 ± 0 .82)mmol/L .The prevalence was 53 .49% in male , and 34 .93% in female .The prevalence was higher among the group of 35- <45 years old for male and 55- <65 years old for fe-male .The level of HDL-C was low among young people .There was aggregation of risk factors among the participants with dyslipi-demia .Non-conditional logistic regression analysis showed that the risk factors were age (OR=1 .701) ,overweight (OR=5 .560) , abdominal obesity(OR=2 .398) ,smoking(OR=0 .545) ,intake of greasy diet(OR=5 .313) ,sleep quality(OR=2 .005) and diastolic blood pressure(OR=3 .061) .Conclusion Lipid disorders becomes a serious problem in the health examination population ,measures such as rational diet ,weight control ,sleep improvement ,pressure control and quiting smoking must be taken .

This research adopted silt as the sample,and the five highest hydrogen production performing strains contained in the sample were isolated. The strain whose hydrogen production was the highest was identified as Enterobacter cloacae by the analysis of 16S rDNA sequencing and comparison. It is showed by Plackett-Burman Experimental Design that only glucose,citric buffer and reducing agent had significant effects on hydrogen production by Enterobacter cloacae FML-C1. The path of steepest ascent was undertaken to approach the optimal response region of those three factors. Central Composite Design(CCD) and Response Surface Methodology(RSM) were employed to investigate the interaction of the variables and to ascertain the optimal values of the factors,which finally led to the maximum hydrogen production(VH2) . The theoretical optimal medium conditions were:glucose 21.5 g/L,citric buffer 13.6 mL/L,reducing agent10.0 mL/L. The five tentative tests matched this model well. The final VH2 was up to 2347.4 mL/L,which was 127.42% enhanced in comparison to the original. The result shows that PB experiment design and RSM analytical method work well in selecting factors which have significant influences on the hydrogen production and,moreover,achieve the ideal optimal result.