Objective:To investigate the mediating effect of marital attitude on the relation between self-esteem and romantic relationship satisfaction in college students.Methods:Totally 342 college students in love were assessed with the Self-esteem Scale (SES),Marital Attitude Scale (MAS) and Quality of Relationship Index (QRI).The Bootstrap method was used to explore the mediating roles of marital attitude between self-esteem and romantic relationship satisfaction.Results:There were significantly positive correlations among the scores of SES,MAS and QRI (r =022-0.95,Ps ＜ 0.01).Self-esteem could directly predict college students'romantic relationship satisfaction (β =0.45,95 ％ CI:0.27-0.63),and had significant positive predictability to college students romantic relationship satisfaction when the marital attitude was controlled (β =0.17,95％ CI:0.08-0.29).Conclusion:It suggests that marital attitude may have a partial mediating effect between self-esteem and romantic relationship satisfaction in college students.

Objective:To explore the moderating effect of gender between love relationship satisfaction and empathy in college students.Methods:Totally 175 pairs of college couples were enrolled.They were assessed with the Quality of Relationship Index (QRI) and Interpersonal Reactivity Index-C (IRI-C) to evaluate love relationship satisfaction and empathy.IRI-C included 4 dimensions named perspective taking (PT),personal distress (PD),fantasy (FS) and empathetic concern (EC).Multiple regression analysis was used to investigate the moderating effects of gender on relationship between love relationship satisfaction and empathy in college students.Results:The scores of QRI and PT were higher in the males than in the females,but the PD scores were lower in the males [(36.6 ±5.9) vs.(35.2 ±6.0),(10.9 ±3.4) vs.(9.9 ± 3.4),(6.0 ±4.1) vs.(8.1 ±4.3),Ps ＜0.01].The QRI scores were the lowest in the students who were in love for less than 3 months (33.1 ±7.1).The PT scores positively predicted the QRI scores (3 =-0.19),the PD scores multiplied by gender negatively predicted the QRI scores (β =-0.17),and the EC scores multiplied by gender positively predicted the QRI scores (β =-0.21).Conclusion:Male students may experience higher love relationship satisfaction than females.The gender plays a moderating role to the influence of empathy on love relationship satisfaction in college students.

Objective To understand the relationship between spouses' marital quality and their mental health.Method 384 pairs of couples from 28 provinces in China were examined with the Marriage Perception Scale(MPS) and Symptom Checklist 90 (SCL-90).Result ( 1 ) Husbands' scores of couple conflict( 10.85 ±5.02) were significantly lower than wives' scores of couple conflict ( 11.66 ± 5.63 ) (P ＜ 0.05 ).( 2 ) Husbands' seores in the 5 factors of SCL-90 ( symptoms that somatization ( 1.44 ± 0.50 ), interpersonal sensitivity ( 1.57 ±0.54),depression( 1 .50 ± 0.52 ) ,anxiety ( 1.46 ± 0.54 ), panic ( 1.38 ± 0.55 ) ) were significantly higher than wives' in the 5 factors of SCL-90[symptoms that somatization ( 1.52 ± 0.54 ), interpersonal sensitivity ( 1.66 ±0.58), depression (1.59 ±0.59 ), anxiety ( 1.55 ± 0.58 ), panic ( 1.47 ± 0.59 )] (P ＜ 0.05 ).( 3 ) Individual's MPS and its SCL-90,spouses' MPS andits SCL-90 correlated as well( r=-0.372 ～0.239)(P＜0.01 ).Conclusion Couples are similar in their marriage perceptions.Husbands are psychological healthier than their spouses'.Individual marriage perception is correlated with his/her mental health level and his/her spouses' as well.

Objective To analyze risk factors for the perioperative heart failure following hip fracture surgery in the aged patients.Methods The present study included 186 elderly patients(≥ 65 years)who had received hip fracture surgery from August 2009 to Janurary 2014 in our department.Their clinical data were analyzed to define risk factors for perioperative heart failure.Results Factors significantly affecting the perioperative heart failure included age,gender,hypertension,time from injury to operation,general health before injury,pain score,perioperative blood transfusion,perioperative human serum albumin transfusion,fluid infusion during operation,perioperative balance between daily fluid intake and output,perioperative temperature changing and heart diseases.A multivariate Logistic regression analysis revealed that the independent risk factors for perioperative heart failure included general health before injury (OR =21.967,P=0.000),perioperative balance between daily fluid intake and output (OR =19.349,P =0.005),heart diseases (OR =6.009,P =0.006).Conclusions In aged patients undergoing hip fracture repair operations the independent risk factors for perioperative heart failure include general health before injury,perioperative balance between daily fluid intake and output,heart diseases.Perioperative risk assessment and prevention are the keys of better prognosis.

Objective: To analyze the mutations of BRCA1 in 9 Chinese familiar breast cancer patients. Methods: Peripheral blood samples were obtained from 9 patients enrolled from 9 breast cancer families, one normal control, 32 sporadic breast cancer patients and 33 normal donors. DNA extracted from lymphocytes was amplified by polymerase chain reaction (PCR). The 22 exons and partial introns of BRCA1 were screened by PCR denaturing high performance liquid chromatography (PCR-DHPLC) and confirmed by direct sequencing. Results: Among these 9 familiar breast cancer patients, a deleterious mutation was detected in one case in exon 11 (3870delTGTC) which was a 4 base deletion and caused a frameshift in turn. One novel and unique amino acid substitution (E867R) was detected in one case. Eight patients were detected to have a known variation in intron 18 (IVS18+65G→A), and the ratio of this variation detected was 88.9%(8/9). The ratio of this variation was 37.5%(12/32) in sporadic breast cancer patients or 33.3%(11/33) in normal control. This variation was found to be accompanied all the time with a known missense variation in exon 11 (P871L) and a polymorphism in intron 9 (IVS8 57delT). Those three variants were also detected in homozygous in one case, which implies the linkage of the 3 sites. The linkage had not been reported. Two patients had been found with a known polymorphism in exon 13 (S1436S). Another known polymorphism was found in one case (L771L). In addition, intronic variants (IVS2+48C→T, IVS2+133C→T, IVS12+112C→A) were detected. Conclusion: The mutations of BRCA1 in Chinese familiar breast cancer patients are different from the hot spots reported in Caucasian and Jewish. It is important that further study be conducted to seek for specific mutations of this gene or other possible relevant genes in Chinese familiar breast cancer patients.

Experiments with lard show that the addition of 10% crude soya bean oil gives a protective index approaching that obtained by the addition of 0.005% of either propyl gallate or tetrahydroxyl-dimethyl-biphenyl (TDBP). In the presence of cupric or ferric salts, however, the percentage of soya bean oil has to be raised in order to obtain satisfactory protection of the lard against oxidation.Dry mixed rations prepared from wheat flour, soya flour, egg powder, dried carrot powder, sesame seeds, sucrose, salt and a suitable concentration of either crude soya bean oil, refined soya bean oil, lard, or lard plus 10% crude soya bean oil, have been kept at 30℃ for over three years without fat deterioration. The rations containing lard or lard plus crude soya bean oil did not give any indication of rancidity even after 160 weeks at 40℃. Under temperatures of 50? and 60℃, they only became rancid after 90 and 52 weeks respectively. Addition of either propyl gallate or TDBP gives only slight improvement in the keeping quality of the rations compounded with crude soya bean oil or refined soya bean oil, and somewhat greater improvement in the ration made with lard.The wrapping material has been shown to exert a profound influence on the keeping quality of the dry rations. Of the 9 different wrapping materials tested, cellophane has been found to be the best; aluminium foil and gum guaiac-treated paper were found also to be excellent, while waxed paper, propyf gallate-treated paper and ordinary untreated wrapping paper greatly accelerate the appearance of rancidity of the rations.

Objective To systematically evaluate the effectiveness of Chinese medicine(CM) combined with chemotherapy in preventing stageⅡ-Ⅲ colorectal cancer patients from postoperative recurrence and metastasis. Methods Databases of PubMed, EMBase, Cochrane Library, China National Knowledge Information(CNKI), VIP Database, Wanfang Database, and SinoMed were retrieved to collect randomized controlled trials(RCTs) of CM combined with chemotherapy in preventing stageⅡ-Ⅲ colorectal cancer patients from postoperative recurrence and metastasis. And then the quality of the included RCTs was evaluated systematically. Results Seven RCTs involving 700 cases of stageⅡ-Ⅲ colorectal cancer patients were included, 355 patients in treatment group were treated with CM combined with chemotherapy, and 345 patients in control group were treated with chemotherapy alone. The results of Meta-analysis showed that treatment group was superior to the control group in preventing the recurrence and metastasis of colorectal cancer one , 2 and 3 year(s) after the radical surgery, showing statistically significant differences(P < 0.05), while the difference of five-year recurrence and metastasis rate was not statistically significant between the two groups(P > 0.05). At the same time, CM combined with chemotherapy had better effect than chemotherapy alone on prolonging the time for recurrence and metastasis, improving performance status and relieving the symptoms, the difference being significant(P<0.05). Conclusion CM combined with chemotherapy exerts better effect than chemotherapy alone in preventing stageⅡ-Ⅲ colorectal cancer patients from postoperative recurrence and metastasis and onimproving the quality of life of the patients. However, for the low methodological quality of the included trials, the conclusion still needs more large-size sample, multiple-center, and high-quality clinical trials to be confirmed.

Background Retinitis pigmeutosa (RP) is a progressive inheritance disease.It is characterized by highly genetical and phenotypical heterogeneity.With the rapid development of genomics,new methods are applied to the genetic screening of RP.Objective This study was to characterize the clinical features of a Chinese family with autosomal RP and to screen the candidate genes.Methods Twelve members from this family were included in the study.All participants underwent complete ophthalmologic examinations.Targeted-capture next generation sequencing (NGS) based molecular genetic analysis was performed on two patients of this RP family(Ⅱ5,Ⅱ 7).The DNA sample from the two patients was separately sequenced using custom capture gene chip,which includes 59 retinal disease genes.The sequencing results were analyzed by bioinformatics technology.Identified variations were verified in the rest family members by PCR and Sanger sequencing.This study was approved by Ethic Committee of West China Hospital,and informed consent was obtained from the subjects.Results Four members of this family were diagnosed as RP,and the rest were asymptomatic.Missense mutation (c.3065T＞C,p.Phe1022Ser) in USH2A and missense mutation (c.1699G＞A,p.Ala1319Gly) in PDE6A were found in two patients (Ⅱ 5 and Ⅱ7).The variants were not co-segregated with the phenotype of this family.The causative mutation was not found by the targeted-capture NGS based eye disease chip,but it ruled out a large number of candidate genes for RP.Conclusions Our study suggests that targeted-capture NGS based eye disease chip can quickly detect mutations in known RP genes.It can be a new applicable and efficient method for molecular genetic analysis of ocular disease.

From the ethanol extract of Adina rubella;twelve compounds were isolated by silica gel and ODS col-umn chromatography.Their structures were identified by spectroscopic analysis as:5;7-dihydroxy-2-methyl-chromone-7-O-β-D-glucopyranoside(1);scopolin(2);(+)-lyoniresinol-3a-O-β-D-glucopyranoside(3);(6S;7R;8R)-7a-[(β-D-glucopyranosyl)oxy]lyoniresinol(4);harman-3-carboxylic acid(5);lyaloside(6);3-oxoquinovid acid(7);oleanolic acid(8);3-acetyl oleanolic acid(9);quinovic acid 3-O-β-D-glucopyranosyl-28-O-β-L-rham-nopyranosyl ester(10);quinovic acid 3-O-β-D-glucopyranosiyl-28-O-β-D-glucopyranosyl ester(11)and pyrocin-cholic acid 3-O-α-L-rhamnopyranosyl-28-[β-D-glucopyranosyl-(1 →6)-O-β-D-glucopyranosyl]ester(12). Compounds 3;4;6;8-10 were isolated from this plant for the first time.

Objective To evaluate the clinical and histological outcomes in a cohort of chronic hepatitis B (CHB) patients who had histologically confirmed severe liver fibrosis and received lamivudine (LAM) therapy for up to 10 years. Methods Thirty-nine CHB patients with severe liver fibrosis (Ishak fibrosis score≥4) were treated with LAM for up to 10 years. Disease progression liver histological improvement, virological and biochemical responses were evaluated during follow-up. Data were analyzed using paired t test, Fisher exact test and Willcoxon test. Results Twenty-eight patients completed the 10-year follow-up. There were 5 (17.9% ) patients with disease progression.At the end of follow up, 16 patients received a second liver biopsy, which showed significant improvement of histological activity index (1.1 ± 1.4 vs 7. 1 ± 3.2, t =- 0.82, P＜0.01 ) and Ishak fibrosis score (3.6±2.2 vs 5.3±0.7, t= -2.89, P＜0.05) compared to baseline. There were 3 cases with Ishak fibrosis score improved from F5 to F0. Among 27 patients, 3(11% ) cases achieved hepatitis B surface antigen (HBsAg) loss and 2 (7 % ) achieved HBsAg seroconversion. At the end of follow-up, 19 out of 23 (83% ) hepatitis B e antigen (HBeAg) positive patients obtained HBeAg loss and 9 (39 % ) obtained HBeAg seroconversion. During LAM treatment, 11 patients experienced virological breakthrough or detected documented LAM-related resistance mutation. The viral loads of all patients were below 1 ×103 copy/mL at the end of follow-up after rescued by add-on or switch to another nucleotide analog.Conclusions Long-term LAM therapy can delay the disease progression in CHB patients with severe liver fibrosis, increase HBsAg and HBeAg loss rates, sustain suppression of HBV replication at a low level and even totally reverse the liver fibrosis in some patients. The effect of LAM resistance mutation on disease outcomes would be reduced by rescue therapy.