Objective To investigate the clinical features of polypoidal choroidal vasculopathy (PCV) in Chinese patients. Methods The clinical data of 71 continuous patients (142 eyes) who were diagnosed with PCV by fundus photography, fundus fluorescein angiography (FFA), indocyanine green angiography (ICGA) and optical coherence tomography (OCT) were analyzed retrospectively. Results Eleven patients (11 eyes) of 71 patients (142 eyes) were diagnosed with PCV to make up 15.49% and 7 75% of the numbers of patients and affected eyes respectivery. The patients included 7 males (63 6%) and 4 females (36 4%). The predominant location for these lesions was the macular region in 10 eyes (90 9%). Fundus examination demonstrated the reddish-orange nodular elevations in 6 eyes. ICGA revealed umbrellalike or twiglike branching vascular networks and polypoidal dilations at the vascular terminals of the branches in all patients, and feeder vassels within choroidal vascular networks were found in 8 eyes. OCT images of retinochoroidal structures showed prominent anterior protrusion of the orange subretinal mass corresponding to the polypoidal structure in the indocyanine green angiogram. An apparent discontinuity was observed in the highly reflective layer which delineates the polypoidal structure. Conclusions PCV mainly affects the male over 50 years and the eyes involved were mostly unilateral. Most of polypoidal vascular lesions are present in the macular area.

The postnatal development of the efferent projections to the lumbosacral spinal cord from the brain stem of rats were studied with HRP retrograde transport method. Fifty per cent solution of HRP was slowly injected into the intumescentia lumbalis on its right side in 18 albino rats from postnatal 1-35 days. The HRP labelled cells were found in the following nuclei:1. Of the midbrain: the nucleus ruber, substantia nigra and nucleus raphe dorsalis.2. Of the pons: the nucleus reticularis pontis oralis, nucleus reticularis pontis caudalis, locus coeruleus, nucleus subcoeruleus, nucleus raphe magnus, nucleus vestibularis lateralis, and nucleus vestibularis medialis.3. Of the medulla oblongata: the nucleus raphe obscurus, nucleus raphe pallidus, nucleus reticularis ventralis, nucleus reticularis gigantocellularis, nucleus reticularis lateralis, nucleus tractus spinalis nervi trigemini, nucleus commissuralis (Cajal), nucleus prepositus hypoglossi, and nucleus gracilis.The number of the labelled cells in the above nuclei increased steadily with age. The results of present study showed that there was a postnatal developmental process in the efferent projections from the brain stem to the lumbosacral cord. The maturity of the projections from ruber nucleus and locus coeruleus were later than those of the vestibulospinal and reticulospinal tracts. The postnatal developmental process of the brain stem projections to the lumbosacral spinal cord were completed by one month after birth.

An intercostal nerve-spinal cord implantation and semitransection of the spinalcord at the point between the origin and insertion of the intercostal nerve was madeon ten adult dogs and two monkeys.One to two weeks after this operation,paraly-tic legs following semitransection of the spinal cord recoverded from the paralysis.The implanted nerves were cut during the different period from 124 to 366 days.The function of the legs of four animals which had recovered paralysed again aftersurgical section of implanted nerve.Histologically,the inserted nerves in the spinalcord were recognized in these animals.Many of the growing fibers extended fromdistal end of the inserted intercostal nerve into the gray and white matter ofthe distal segment of the spinal cord.It is considered that the efficacy of the inter-costal nerve-spinal cord implantation depends upon the choice of the symptomaticindications and the success of the implantation procedure.

0.05 ).On the 5th day after radiation,the body weight in +Gln or -Gln groups was significantly lower than that in control group( P

Objective To observe the effect of glutamine on gut barrier function and antioxidative capacity in rats with radiation damage. Methods A total of 30 healthy Wistar male rats were randomly divided into three groups: control group, GLN enriched group(+GLN) and GLN free group( GLN). Rats in control and GLN group were fed with a basic diet, while 3% GLN was added into the diet of the +GLN group. On the 15th day, rats in GLN and +GLN groups received 9.0 Gy 60 Co ? irradiation in total body. The rate of the gut bacterial translocation and the level of endotoxin, GSH and SOD in the serum were determined at the end of the experiment. Results The rate of bacterial translocation and serum endoxin level were significantly lower in +GLN than in GLN, and GSH and SOD levels in serum were significantly higher in +GLN than in GLN. Conclusion Glutamine supplement is helpful to protect the gut barrier function and improve the antioxidation capacity in rats with radiation injury.

Focus on the defects of the extraction method of velocity pulsated signals in the velocity spectrum image of pulsed Doppler ultrasounds (such as the limited data, great amount of work and low reproducibility), we combine the earlier research results and the basic rules of the velocity spectrum image, and employing the visual basics plateau, to design and develop a software to extract the velocity signals from the spectrum image automatically. Compare the mean velocity and RNS between the PIV and the PDU resulting from the method, the correlations are r = 0.93 and r = 0.78 respectively. The study reveals the software increase the amount of valid data, decrease the amount of work and increase reproducibility of the signals extraction. The method may be an effective way to analyse the velocity spectrum image of PDU in clinic.
Arteries ; diagnostic imaging ; Blood Flow Velocity ; physiology ; Humans ; Pulsatile Flow ; Signal Processing, Computer-Assisted ; Software ; Ultrasonography, Doppler, Pulsed ; methods

This paper summarized the characteristics, rehabilitation needs, and situation and problems of stroke dysfunction in China. Approaches and methods of family rehabilitation were also discussed.

The effects of tissue factor (TF) on doxorubicin-induced apoptosis in human neuroblas- toma were investigated. The expression of TF was examined by Western blotting. TFsiRNA-pSUPER plasmid was constructed by inserting specific 19-nt silencing sequence targeting TF gene into pSU- PER vector. Transfection of TFsiRNA-pSUPER was performed using lipofectamine2000. The cytotox- icity of doxorubicin was determined by WST assay. The activation of Caspase-3 and PARP induced by doxorubicin was tested by Western blotting. The apoptotic cells were stained by Hochest33342 and counted under fluorescence inverted microscope. It was found that human neuroblastoma cell line SK-N-MC expressed high level of TF. Knockdown of the TF expression was achieved by trans- fection of TFsiRNA-pSUPER on SK-N-MC cells in a dose-dependent manner. Inhibition of TF sig- nificantly decreased the viability of transfected SK-N-MC cells treated with different concentrations of doxorubicin. Cleavage of Caspase-3 and PARP was enhanced in transfected SK-N-MC cells with down-regulation of TF. TFsiRNA treatment significantly increased the number of apoptotic cells in transfected SK-N-MC cells as compared with those control cells (P＜0.05) when these cells were ex-posed to 1 μg/mL doxorubicin for 8 h. These results suggested that knockdown of the TF expression by specific siRNA vector could increase the cytotoxicity of doxorubicin and enhance doxorubi- cin-induced apoptosis in human nearoblastoma cells. Over-expression of TF might contribute to chemotherapy resistance in human neuroblastoma and its progression, at lest in part, by regulating doxorubicin-induced apoptosis.