Bone tissue engineering is one of the most clinical applicable research areas in tissue engineering. Seed cell is the first step and essential element of construction and application of tissue-engineered bone. In recent years, many inspiring achievements have been gained in the field of seed cell study. In this review, current status and the prospects of study of seed cell source in bone tissue engineering are reviewed.

Objective To investigate the clinical outcomes of microwave heliotherapy in situ on the primary or metastatic malignant tumors of the pelvis.Methods From February 2000 to April 2009,18 patients with primary or metastatic malignant tumors of the pelvis were treated with microwave heliotherapy in situ,and followed a total or partial tumor resection.There were 11 males and 7 females with an average age of 45 years(range,16-72).Twelve cases were diagnosed as primary malignant tumor and 6 as metastases.Locations of tumors involved:the Ⅰ region 6 cases.the Ⅱ region 10 cases.and the Ⅲ region 2 cases.The exposures of all tumors were via aTtype or ilioinguinal approach.The lesions were heated at 50℃ for 20 min by 2450 MHz microwave,with surrounding soft tissue protected by copper.mesh.The necrotic tumor tissues were total or partial excised after treatment,with preservation of the anatomical continuity of the pelvic ring.Results The duration of surgery was 60-180 min (110 min on average).The blood loss was 400-800ml(480 ml on average).All patients were followed-up for 0.5-7 years(3.5 years on average).Tumor local recurred in 1 case with chondrosarcoma,and was survival in tumor-bearing after 6 months follow up.One case with malignant fibrous histiocytoma died due to brain,pulmonary,and all body metastases.One case with osteosarcoma died due to pulmonary metastases.Five cases with the metastases died due to non-pelvis metastases.Functions of hip joint in 18 patients were as follows:flexion 80°-130°,extension 0°-10°,abduction 25°-35°,and adduction 18°-23°.Conclusion The clinical result demonstrated that the advantages of microwave heliotherapy in situ were quick increase of temperature,sensitive responses,easy control of temperature,and effective inactivation of tumor cells in the malignant bone tumors of pelvis.

By introducing the historical development and current situation of American anesthesia specialist nurses, including qualification certification, curriculum setting and assessment, professional scope and use, work content and effectiveness, this paper analyzes the experience and lessons of American anesthesia specialist nurses, combined with the current situation of anesthesia specialist nurses in China, analyzes the reference aspects of our country, and puts forward suggestions to the standardized development of anesthesia specialist nurses in China at the present stage.

Objective:To explore the application effect of high-efficiency steam cleaner in cleaning cerebral pressure tube.Methods:A total of 3 000 cerebral pressure tubes recovered in the First Department of Disinfection and Supply Center of the First Affiliated Hospital of Zhengzhou University from October 1, 2019, to March 31, 2020, were selected for analysis. According to different cleaning methods, they were divided into the observation group, the control group A and control group B, with 1 000 in each group. The observation group used enzyme solution immersion combined with the high-efficiency steam cleaner to clean, the control group A used enzyme solution immersion to clean, and the control group B used enzyme solution immersion combined with the ultrasonic cleaner to clean. The cleaning time, qualified cleaning quantity, cleaning safety, residual gas and item damage of the three cleaning methods were compared.Results:The cleaning time of cerebral pressure tubes in the observation group was (8.75±1.16) min, which was lower than that of the control group A (12.38±1.35) min and the control group B (10.17±2.09) min, and the differences were statistically significant ( P<0.01) . The number of qualified and safe cleaning of cerebral pressure tubes in the observation group was more than those of the control group A and the control group B, and the differences were statistically significant ( P<0.05) . The amount of residual gas and the number of goods damaged of the cerebral pressure tubes in the observation group were less than those of the control group A and the control group B, and the differences were statistically significant ( P<0.05) . Conclusions:The high-efficiency steam cleaner has a better effect on cleaning the cerebral pressure tube and is suitable for popularization and application.

Objective:To explore the influencing factors of psychological resilience in breast cancer patients undergoing postoperative chemotherapy.Methods:From January 2019 to June 2020, convenience sampling was used to select 210 patients with breast cancer undergoing postoperative chemotherapy in the First Affiliated Hospital of Zhengzhou University as the research object. The patients were investigated with the General Information Questionnaire and the Connor-Davidson Resilience Scale (CD-RISC) . Taking 70 scores as the critical value of CD-RISC, patients were divided into the good psychological resilience group (CD-RISC score≥70) and the poor psychological resilience group (CD-RISC score<70) according to their scores. Binomial Logistic regression analysis was used to analyze the influencing factors.Results:There were 112 patients in the good psychological resilience group and 98 patients in the poor psychological resilience group. The univariate analysis showed that there were significant differences in the psychological resilience of breast cancer patients undergoing postoperative chemotherapy with different marital status, per capita monthly income of family, course of chemotherapy, postoperative complications, and family residence ( P<0.05) . The binomial Logistic regression analysis showed that marital status, per capita monthly income of family, course of chemotherapy, postoperative complications, and family residence were the influencing factors of psychological resilience in breast cancer patients undergoing postoperative chemotherapy ( P<0.05) . Conclusions:There are many influencing factors of psychological resilience in breast cancer patients undergoing postoperative chemotherapy. Targeted psychological intervention should be carried out according to the influencing factors to improve the psychological resilience of patients.