Objective To establish a high performance liquid chromatographic method for determination of 6-methyl coumarin in cosmetics. Methods Samples were extracted with ethanol, On C18 column CH3CN-0.02 mol/L NaH2PO4(pH=3.5, 35∶65) was applied as mobile phase, 6-Methyl Coumarin in cosmetics was determined by photo-diode-array(PDA) detector. Results The linear range of the method was 1.0-25.0 mg/L. The lowest detected limit of 6-Methyl Coumarin in 1.0 g samples was 0.000 1%. The recovery rates were 96.6%-99.2%, and the relative standard deviation were 2.0%-4.4%. Conclusion The method was simple, rapid and accurate, which was suitable for determination of 6-methyl coumarin.