1.The expression level of miR-125a-5p in colorectal cancer with various differentiation
Xiaobing WU ; Xuehu XU ; Shangbiao WU ; Qingping JIANG ; Haibo LIU ; Rong CHEN ; Yan SUN
Cancer Research and Clinic 2014;26(12):801-804
Objective To identify the expression level of miR-125a-5p in colorectal cancer (CRC) with various differentiation,and to investigate its diagnostic significance.Methods Real-time polymerase chain reaction was used to analyze the expression level of miR-125a-5p in tumors and paired normal tissues of different differentiated CRC.Receiver-operating-characteristic (ROC) curve was performed to evaluate the specificity and sensitivity of using miR-125a-5p as biomarkers to discriminate CRC patients from normal persons.Results Compared with normal tissues,miR-125a-5p was down-regulated in both high and moderate differentiation,miR-125a-5p could distinguish CRC from normal persons with high sensitivity and specificity,which were 80.00 % and 90.00 %,respectively.Conclusions miR-125a-5p down-regulated in CRC indicates that miR-125a-5p contributes to tumorigenesis,and may be a potential biomarker for CRC.
2.Study on miR-490-5p and miR-363 as novel biomarkers for the diagnosis of colorectal cancer.
Xuehu XU ; Xiaobing WU ; Shangbiao WU ; Qingping JIANG ; Haibo LIU ; Rong CHEN ; Yan SUN
Chinese Journal of Gastrointestinal Surgery 2014;17(1):45-50
OBJECTIVETo identify dysregulated microRNA(miRNA) that can act as novel biomarker for colorectal cancer screening.
METHODSReal time-polymerase chain reaction was used to detect the expression profile of miRNA in tumor and paired normal tissues, and the significant dysregulated miRNA was examined by non-paired t-test. Receiver operating characteristic(ROC) curve was performed to evaluate the specificity and sensitivity of miR-363 and miR-490-5p as biomarkers to discriminate colorectal cancer patients from normal person.
RESULTSSeven-three dysregulated miRNAs were found in male samples, of which 6 miRNAs were up-regulated and other 67 miRNAs were down-regulated, while 42 dysregulated miRNAs were found in female samples, of which 5 were up-regulated and 37 were down-regulated in tumor tissues compared with normal tissues. Among above dysregulated miRNAs, 33 miRNAs had significant differences, besides, dysregulated expression level of 10 of 33 miRNAs was over 5 folds in male and female as well as mixed samples.
CONCLUSIONSThe expression pattern in female is different from that in male. miR-363 and miR-490-5p possess the potential in screening colorectal cancer patients from healthy people.
Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor ; analysis ; Case-Control Studies ; Colorectal Neoplasms ; diagnosis ; Early Detection of Cancer ; Female ; Humans ; Male ; MicroRNAs ; analysis ; Middle Aged ; Sensitivity and Specificity ; Young Adult
3.Rapid identification of Oncomelania hupensis snail habitat in the Poyang Lake region by Geographic Information System (GIS) and Remote Sensing (RS).
Jiagang GUO ; Dandan LIN ; Guanghan HU ; An NING ; Hongyun LIU ; Shangbiao LU ; Dong LI ; Xiaohua WU ; Rongrong WANG ; Minggang CHEN ; Jiang ZHENG ; Marcel TANNER
Chinese Journal of Epidemiology 2002;23(2):99-101
OBJECTIVETo identify Oncomelania hupensis snail habitats and areas with high transmission potential by GIS/RS.
METHODSMarshland areas near high endemic villages of schistosomiasis in the Poyang Lake region were selected. Corresponding map was digitized and (Landsat 5 TM) image was corrected according to the digital map. The image in dry seasons was calculated by both normalized difference vegetation index (NDVI) and tasseled cap model.
RESULTSResult showed that snails spots were distributed in class 6, 7 and 8. Farther analysis of both NDVI and tasseled cap model showed that the snail habitats were mainly distributed in the areas where NDVI value was more than 110, and in tasseled cap wetness value between -10 to 3 with correction rate 94.93%.
CONCLUSIONFirst step was to use unsupervised classification to define the class 6, 7 and 8 snail habitat environment. Second step was to extract the value by NDVI model, and to define a healthy vegetation as snail suspicious habitat when NDVI value was more than 110. Then the third step was to use tasseled cap wetness model to define the areas as snail habitats which value was between -10 to 3.
Animals ; Demography ; Disease Vectors ; Schistosomiasis ; transmission ; Snails
4.Role of centromere protein H in human gastric cancer cell proliferation.
Tianyi QUAN ; Benfu HE ; Tiejian LIU ; Weiming LI ; Shangbiao WU ; Qingping JIANG ; Weiqiang LIU ; Haibo LIU ; Xuehu XU
Journal of Southern Medical University 2012;32(2):265-269
OBJECTIVETo explore the role of centromere protein H (CENP-H) in the proliferation of human gastric cancer cells.
METHODSRT-PCR and Western blot analysis were employed to examine the mRNA and protein expressions of CENP-H in 7 human gastric cancer cell lines and immortalized human gastric epithelial cells (GES-1). The cells were infected with the retrovirus vectors pMSCV-CENP-H or CENP-H-RNAi to establish stable cell lines with high CENP-H expression or CENP-H expression interference. MTT assay and colony formation assay were used to examine the changes in the cell proliferation after the infection.
RESULTSCENP-H was over-expressed in gastric cancer cell lines AGS, BGC823, SGC-7901, MKN45, HGC27, MGC-803 and MKN28 at both mRNA and protein levels. The established AGS/CENP-H cell line with increased CENP-H expression showed enhanced proliferative activity, while the cell line MGC-803/CENP-H-RNAi with CENP-H expression interference showed an obviously lowered proliferation ability.
CONCLUSIONCENP-H promotes the proliferation of human gastric cancer cells, suggesting its important role in the occurrence and development of gastric cancer.
Cell Line, Tumor ; Cell Proliferation ; Chromosomal Proteins, Non-Histone ; genetics ; metabolism ; Humans ; RNA, Messenger ; genetics ; metabolism ; Real-Time Polymerase Chain Reaction ; Stomach Neoplasms ; metabolism ; pathology