AIM: To observe the functional recovery of knee joint in patients with posterior cruciate ligament (PCL) under knee arthroscopy with ligament advanced reinforcement system (LARS) artificial ligament transplantation. METHODS: From January 2005 to May 2006, six patients with PCL injury treated by reconstruction of PCL with LARS artificial ligament transplantation were selected from the Department of Orthopedics, Huadu Hospital Affiliated to Southern Medical University. All subjects agreed with the operation before the surgery and all reconstruction surgeries were of second period. ① The tibial tunnel was established firstly. ② Then was the femoral tunnel. ③ Under knee arthroscopy, the "Y"-shape double cords of LARS artificial ligament were cut through ante-out and meta-in bone tunnels of femur, and fixed at 90o and 30o flexion respectively. Patients were required to motor 3 days postoperative, and patients actively exercised their quadriceps muscle of thigh at one week later. Regular follow-ups were conducted postoperatively, and functional evaluation of the knee joints was performed by according to Lysholm standards, including 8 aspects: Limping, supporting, interlocking, unstable, painful, swell, upstairs and downstairs and squatting with a total score of 100 points. The higher the score was, the better the functional recovery of knee joint. RESULTS: All of the 6 patients were available at follow-up, and no one withdrew from the study. The follow-up was conducted at the 7th, 7th, 10th, 10.4th, 12.8th, and 14th months with an average duration of 10.2 months. After operation, unstable symptoms of knee joint in all subjects disappeared with negative sag sign and posterior drawer. The Lysholm knee scores of patients postoperation were significantly higher than those before the surgery (83.5?4.5, 51.6?3.6, P

Castleman's disease is a rare disorder of the lymphoid system characterized by noncancerous growths that may develop in lymph node tissues throughout the body. The incidence of this disease has been increasing in recent years. Because of the difficulty of diagnosis and high-misdiagnosis, more and more clinicians have paid close attention to this disease. By the clinical work, and with the help of related reports, the preliminary summary of this disease is made.

Matrine is an effective natural product isolated from the traditional herb,Sophora flavescens,with wide range of pharmacological and clinical effects and treating as the treatment for chronic viral hepatitis in clinic.Currently,the mainstream clinical preparations of matrine involved injections,tablets,capsules and suppositories,while preparations under development included targeting preparations,sustained-release preparations and transdermal drug delivery preparations.Admittedly,It can significantly improve the biological utilization of matrine through the optimization of its clinical preparations for with scientific researches and the development of new dosage forms.While the clinical applications will be expanded and the clinical efficacy of the preparations of matrine enhanced with the development of researches for new dosage forms and drugs and the application of new materials.It may be also benefical to the study of monomer compositions of Chinese material medica,including the development of the preparation of matrine.Rested on the recent studies,pharmacological activities,preparation methods and researches for the preparations of matrine was summarized in this text for providing a reference for developing its preparations in the future.

The medication characteristics of doctor ZHENG Chong-guang in Qing Dynasty was studied from prescription metrology range index,including properties,tastes,channel tropism and function index.It was found that his clinic prescription practice was different from other doctors in Jiangzhe province which originated from his territory character,the territory character of Xin'an school was disclosed,it was suggested that prescription metrology range index was an valuable impersonal index for doctor's territory character.

With the technology progress of human genome sequencing and biomedical analysis and the emergence of big data analysis tools,the new concept of precision medicine has been put forward.Clinically,the application of precision medicine becomes more and more widely in personalized medicine,genetic disease analysis and disease prediction,etc,which will be the trend of disease diagnosis and treatment in the future,however,precision medicine is based on the precise detection.The requirement of the precise detection promotes the rapid development of new detection methods.Clinical laboratory is the carrier to achieve accurate detection,we need to strengthen the construction of clinical laboratory and improve the management level of the laboratory,so as to achieve accurate detection,to provide a more effective basis for the diagnosis and treatment of precision medicine.

Lung cancer is the most common malignant tumor globally, with the highest incidence as well as mortality in China. Absence of the effective screening method for early detection results in the high mortality. Five-year survival rate in patients with advanced cancer decreases remarkably compared with that in patients with early stage disease. Hence, the early detection of lung cancer is of vital importance. DNA methylation has close correlation with the initiation and development of tumor genesis. With the improvement in DNA methylation, aberrant DNA methylation has been identified in lung cancer. Detection of methylation in the specimens, such as tissue, bronchoalveolar lavage fluid, serum or plasma, sputum and urine, contributes to the early detection and improvement in the prognosis and treatment of lung cancer.

Objective To investigate the iodine nutritional status of the crowd that did not participate in the iodine deficiency disorders (IDD) survey in iodine deficiency areas in Jiangsu Province so as to provide a basis for prevention and control of IDD.Methods Eight to ten years old children were chosen from the counties(cities,districts) of Jiangsu Province that did not participate in the IDD survey and urine samples of students and salt samples of their families were researched.In each county (city,district) one township (street) was selected according to 5 directions of east,south,west,north,center; in each township one primary school was selected and in each primary school 20 children aged 8-10 were chosen half of the male and female,to collect their urine samples and salt samples at their families.Urinary iodine was measured using arsenic cerium catalytic spectrophotometric method (WS/T 107-2006),and salt iodine was measured using direct titration of universal test method in salt industry (GB/T 13025.7-1999).Iodine nutrition criterion of international health organization recommended staindard:children urinary iodine ＜ 100 μg/L as iodine deficiency,100-199 μg/L as iodine appropriate,200-299 μg/L as iodine more than appropriate,≥ 300 μg/L as iodine excess.Results A total of 7 523 urinary samples of 8-10 years old children were collected from 76 iodine deficiency counties (cities,districts),and the median of urinary iodine was 235.0 μg/L.In all investigated counties (cities,districts),the medians of urinary iodine of 22 counties(cities,districts) were 100-199 μg/L,42 counties(cities,districts) were 200-299 μg/L,and 12 counties (cities,districts) were ≥300 μg/L.A total of 7 523 salt samples were collected,and the median of salt iodine was 29.68 mg/kg; Taizhou City had the highest salt iodine median,which was 31.43 mg/kg; and Lianyungang City,the lowest,25.11 mg/kg.The coverage rate of iodized salt was 98.6％(7 417/7 523); iodized salt qualification rate was 98.6％ (7 312/7 417); and consuming rate of qualified iodized salt was 97.2％(7 312/7 523) in 76 iodine deficiency counties (cities,districts).Conclusions The progress in prevention and control of IDD in iodine-deficient areas that did not participate in IDD survey in the past is good in Jiangsu Province; the overall level of iodine nutrition of whole population is adequate.But the median of urinary iodine in some counties (cities,districts) is at a higher level.

Objective To master iodine intake ways of children in Yancheng (coastal) and Zhenjiang (inland) cities,analyze their urinary iodine difference and provide solid evidence for adjustment of salt iodine and adequate iodine intake level of local residents.Methods Totally 50％ counties were randomly selected in Yancheng and Zhenjiang cities and 5 towns were sampled in each county in 2014,local water iodine,domestic salt iodine,local eating habits and urine iodine of children 8-10 years old were investigated,and the influences of different iodine intake ways on urine iodine level were analyzed.Results The median of water iodine was 4.76 μg/Lin Zhenjiang (n =40) and 50.80 μg/L in Yancheng (n =50),the difference was statisticelly significant in two cities (Z =6.93,P ＜ 0.05).Totally 2 400 salt samples were tested,the mean of salt iodine was (24.20 ± 5.07) mg/kg in Yancheng (n =1 500) and (22.87 ± 2.77) mg/kg in Zhenjiang (n =900),the difference was statisticelly significant in two cityies (t =7.23,P ＜ 0.05).The salt intake level in Zhenjiang (10.69-± 9.00) g/d appeared to be higher than that in Yancheng (8.40 ± 3.57) g/d,but the difference was not statistically significant (t =1.62,P ＞ 0.05).Totally 1 200urine samples were tested,the urine iodine level of Zhenjiang got higher results (265.83 μg/L,n =450) than that of Yancheng (182.15 μg/L,n =750,Z =12.18,P ＜ 0.05).The ratio of water iodine intake/salt iodine intake was about 1 ∶ 4 in Yancheng and 1 ∶ 50 in Zhenjiang,while residents in Zhenjiang got 21％ of daily iodine through extra ways.Eating habits like eating out did not have significant impact on urine iodine.Conclusions Based on the water iodine level,Yancheng is considered to be a appropriate iodine level area while Zhenjiang is a lack region.However,Zhenjiang has a higher urine iodine level than Yancheng,which is more than adequate to the recommendation.Salt iodine is a main iodine source in both cities.In iodine appropriate and deficiency areas,eating habits and food choices may play a more important role than geochemical factors in iodine intakes among residents.

Objective In this study ,we constructed a series of recombinant plasmids carriers expressing shRNA targeting hTERT and Bi‐1 gene .These recombinant plasmids carriers were transfected into CNE‐2Z cell lines using Lip and continuously in‐duced the expression of shRNAs .Furthermore ,the shRNAs caused the degradation of mRNAs homologous in sequence with the target genes ,which lead to a sequence‐specific gene silencing .Methods The CNE‐2Z cells was divided into untreated group ,pEG‐FP‐N1 group and pEGFP‐N1/Lip group .Flow cytometry(FCM ) was applied to determine the transfection efficiency .The changes of hTERT and Bi‐1 gene expression were detected by Real‐time RT‐PCR and Western blotting .Results The best transfection effi‐ciency between plasmid and Lip was 2 .5 μg plasmid and 6 .25μL Lip .Conclusion We constructed several shRNA recombinant eu‐karyotic expression plasmids successfully .The recombinant plasmid can inhibit the expression of hTERT and Bi‐1 gene specifically and effectively .