Objective:To examine the quality of the donor corneal tissue preserved with Optisol.Methods:An auto-designed bottle underprop was installed on the chin underprop of slit lamp microscope,and it was applied to examine the quality of donor corneal tissue preserved in bottle with Optisol.Results:The donor corneal layers preserved in bottle with Optisol could be observed clearly from endothelial layer to epithelial layer by improved slit lamp microscope.Conclusion:An effective examining method is supplied to assure the quality of donor corneal tissue from eye bank.

Background Previous studies showed that microRNA-184 (miR-184) might inhibit the biological function of vascular endothelial cells.In ophthalmology,to determine the inhibitory effect of miR-184 on angiogenesis is of clinical significance for the prevention and treatment of corneal inflammation,retinal and choroidal neovascularization.Objective This study was to verify the influence of miR-184 transfection on the proliferation,migration and angiogenic ability of HUVECs and explore its mechanism.Methods HUVECs line was cultured in vitro and divided into 4 groups.No transfection regent and RNA molecule were added in the medium of the blank control group;only transfection regent was added in the blank transfection group; hsa-miR-184 mimic/negative was added in the negative control group,and Cy3 labeled hsa-miR-184 mimic-lipofectamine 2 000 mixture was added in the miR-184 transfection group.The proliferation of HUVECs (absorbance,A490) was detected by MTT.The transmembrane cell number was counted by Transwell insert to evaluate the migration ability of HUVECs.A three dimensional cultural system of cells was constructed on the matrigel,and tube formation number of HUVECs was assessed.Results Positive HUVECs for Cy3 labeled siR-RiboTM + hposome showed red fluorescence,with the optimal transfecting concentration ＞ 100 nmol/L.The relative expression levels of miR-184 were 1 524.10±385.89 and 1.00±0.05 in the miR-184 transfection group and the negative control group,showing a significant difference (t=-7.894,P＜0.01).The A490value of HUVECs was 0.50±0.04 and the number of migrating cells through the transwell membrane was (55.40±5.86)/field in the miR-184 transfection group,and they are significantly reduced in comparison with (0.62±0.04) and (83.40±5.59)/field in the negative control group (t =5.639,7.730,all at P＜ 0.01).A significant difference was found in the total branch points of capillary tubes between the miR-184 transfection group and negative control group ([13.33 ± 2.08]/field versus [22.00 ± 2.00]/field) (t =5.511,P＜ 0.05).Conclusions MiR-184 can suppress the proliferation,migration and tubing of HUVECs after transfection in vitro.MiR-184 participates in the regulation of angiogenesis.

Objective To investigate the effects of 1,25-dihydroxyvitamin D3 in the prevention of corneal allograft rejection. Methods Forty-five SD rats (only right eyes) were used as recipients,and 15 Wistar rats (double eyes) were used as donors. SD rats were randomly divided into 3 groups: the autograft control group,the allograft control group (receiving peanut oil as placebo),and the allograft group were treated with 1,25-dihydroxyvitamin D3 (1.0 ?g.kg-1.d-1,3 times/d for 13 d,i.p.). Drugs were injected intraperitoneally for 14 d after transplantation. Routine antibiotic eye drops were used. Grafts were observed by operating microscopy everyday after transplantation. Five SD rats in each group were killed respectively at the 14,21,and 30 d postoperatively. Neovascularization and inflammation were evaluated with HE staining. ELISA was used to detect the contents of IL-1?,IL-2,IL-8,and IL-10 in the peripheral blood. Results The mean survival time (MST) of the graft was (21.7?6.8) d in the autograft control group,(11.2?2.5) d in the allograft control group,and (19.3?5.2) d in the allograft group. There was statistically significance between the latter 2 groups (P

Dry eye is one of the common chronic disease of ocular surface characterized by the stability of tear film decrease and ocular surface lesion .The change of tear film and the inflammatory reaction which based on immune and the neural regulation abnormality are the important factors in the occurrence , development of dry eye .Alterations in gene structure has become a new research direction , also computer image processing techniques and detection tech-niques of molecular biology gradually have been applying in research and diagnosis of dry eye .

d ultrasound energy can obviously improve plasmid DNA of pEGFP transfect efficiency in neovascularized corneal of rabbit.

Objective To understand the teachers′health status of one university in Chongqing to provide the basis for the healthcare of this group.Methods The teachers′health examination information in1 303 cases of physical healthy examination was retrospectively analyzed.The data were analyzed by the SPSS17.0 statistical software.Results The main detected diseases were conj unctivitis,fatty liver,cataract,refractive error,eyeground arteriosclerosis and hypertension.The top-5 health risk factors includ-ed dyslipidemia,overweight,hyperpiesia,increased uric acid and fasting blood glucose abnormality.The detection rates of conj uncti-vitis,fatty liver,eyeground arteriosclerosis,overweight,hyperpiesia,increased uric acid,fasting blood glucose abnormality examined in males were obviously higher than those in females(P<0.05),while which of cataracts,refractive errors,hypertension and dyslip-idimia were not relevant to gender(P>0.05).Apart from the increased uric acid(P=0.740),the morbidity and risk factors in dif-ferent ages were markedly different(P<0.01).The case rate of diabetes and hypertension was lower than the national average. Conclusion The eye health status of teachers in this university is not optimistic;there were many health risk factors.The health knowledge education of overweight,dyslipidemia,pathoglycemia and hyperpiesia should be carried out aiming at this group.