Alternating hemiplegia of childhood(AHC) is a hereditary disease characterized by hemiplegia spells,abnormal eye movements,dystonia and cognitive impairment.There are three phases of the disease.Each phase has its unique clinical symptoms:phase 1-abnormal eye movements and dystonia;phase 2-hemiplegia spells; phase 3-permanent cognitive impairment.The severity of cognitive impairment depends on the time of onset of hemiplegia spells:the earlier the onset is,the worse outcome will be.No effective treatment has been established.Thetreatments currently include:avoiding predisposing factors and taking drugs such as flunarizine to prevent hemiplegia attacks,in the inter-ictal stage;and sedation during hemiplegia attacks.According to the latest research,AHC is caused by the de novo mutation of gene ATP1A3.

Objective To investigate the effects of Xiaoke Wan on serum adiponectin levels in patients with type 2 diabetes metlitus. Methods A total of 60 2TDM patients with deficiency of both qi and yin syndrome were recruited into a control group and a treatment group randomly, 30 cases in each group. The treatment group was treated with Xiaoke Wan, and the control group was treated with glibenclamide. After 32 weeks, the changes of FPG, 2hPG, HbAIc, FINS, C-P, APN, HBCI,IRI and improvement of symptoms were compared between the two groups. Results Compared with glibenclamide, Xiaoke Wan can significantly improve the patients' symptoms, reduce the level of IRI (t=-1.590, P=-0.036), and increase serum APN (t=1.918, P=0.002). Conclusion Xiaoke Wan can significantly improve symptoms, reduce fasting blood glucose and 2 hour postprandial blood glucose, recover the secretion of adipouectin, decrease insulin resistance in 2TDM patients with deficiency of both qi and yin.

?AlM:To evaluate the effect and safety of 1. 8mm coaxial microincision phacoemulsification - trabeculetomy with ultra-thin intraocular lens ( lOL ) implants for treating glaucoma complicated with cataract, and to compare with the traditional 3. 0mm small phacoemulsification-trabeculetomy with foldable lOL implantion.
?METHODS: ln this prospective study, 36 patients ( 36 eyes) with glaucoma and cataract in lnner Mongolia Autonomous Regian People’s Hospital were collected and randomly divided into 2 groups. For the small incision group: 18 cases ( 18 eyes ) underwent 3. 0mm coaxial incision phacoemulsification-trabeculetomy with foldable lOL implantion; ln th microincision group: 18 cases ( 18 eyes ) underwent 1. 8mm microincision phacoemulsification-trabeculetomy with ultra-thin lOL implantion. The two groups were recorded for 1wk, 1 and 3mo of visual acuity, corneal endothelial cell density, surgically induced astigmatism, intraocular pressure ( lOP ) , filtering bleb and complications. Pearson’s Chi-square test ande t-test were used to determine differences between the two groups.
?RESULTS:At 1wk postoperatively, visual acuity in the microincision group was better than that of small incision group, the difference was statistically significant ( P<0. 05). At 1 and 3mo, the difference in corrected visual acuity between the 2 groups had no significant difference (P>0. 05). At 1wk, 1 and 3mo, there was a significant different between the 2 groups in surgically induced astigmetism (P<0. 05). At 1wk postoperatively, there was a significant difference in corneal endothelial cells density between 2 groups (P<0. 05). But there were no significant difference at 1 and 3mo ( P > 0. 05 ). lOP reduced after surgy ( microincision group: 15. 26 ±3. 12mmHg, small incision group: 14. 57±2. 86mmHg), there was no significant difference between the 2 groups (P>0.05). There was no significant different between the 2 groups in blebs (P>0. 05). Neither iris injury, posterior capsule rupture nor anterior chamber bleeding complications was found in any groups.
?CONCLUSlON: TBy compared with traditional 3. 0mm coaxial small incision phacoemulsification -trabeculectomy with foldable lOL implantion, 1. 8mm microincision phacoemulsification-trabeculectomy withe ultra- thin lOL implantion can effectively reduce the astigmatism operation. This operation is safe, effective, convenient surgy for treating cataract and glaucoma.

Carcinoma, Squamous Cell ; metabolism ; pathology ; Esophageal Neoplasms ; metabolism ; pathology ; Humans ; Mitogen-Activated Protein Kinase Kinases ; metabolism ; NF-kappa B ; metabolism ; Prognosis ; STAT Transcription Factors ; metabolism ; Signal Transduction ; Transforming Growth Factor beta ; metabolism ; Wnt Proteins ; metabolism

The pancreatic islet cell and testicular sertoli cells of rat were alone-and co-microencapsulated and cultured for 11 days, then insulin concentration of culture fluid was detected.The results showed that islet function in co-microencapsulated group was better than that in co-culture of microencapsulated islet and microencapsulated testicular sertoli cells and also better than that single microencapsulated islet group (P<0.05).

Objective To investigate the prostate cancer diagnosis value of prostate-specific antigen (PSA) and free prostate-specific antigen (FPSA) change quantity before and after antibiotic treatment in patients with abnormal PSA.Methods Eighty-five patients with elevated PSA were selected,and the patients were treated with antibiotic (levofloxacin intravenous drip for 1 week).The PSA and FPSA levels were detected before and after treatment.All of the patients underwent prostate needle puncture biopsy.The relation between the PSA,FPSA change quantity before and after treatment and the puncture biopsy positive rate were analyzed.Results The PSA and FPSA levels after treatment were significantly lower than those before treatment:(5.82 ± 1.61) μ g/L vs.(7.71 ± 1.32) μ g/L and (0.80 ± 0.14) μ g/L vs.(0.98 ± 0.17) μ g/L,there were statistical differences (P ＜ 0.05).In 85 patients,10 cases were puncture biopsy positive.After treatment,no PSA change was in 8 cases,PSA decreased ＜ 10％ was in 27 cases,PSA decreased 10％-30％ in 24 cases,PSA decreased 31％-50％ was in 18 cases,and PSA decreased ＞ 50％ was in 8 eases,and puncture biopsy positive were 1 case (1/8),4 cases (14.8％,4/27),3 cases (12.5％,3/24),2 cases (2/18),0case,respectively.Conclusions The PSA can decrease after antibiotic treatment in patients with abnormal PSA,and small decrease of PSA does not mean that the risk of prostate cancer disappears,but the PSA decrease ＞ 50％ after antibiotic treatment means the risk of prostate cancer decreases,which may be closely observed.In patients unable to tolerate or unwilling to accept the prostate needle puncture biopsy,the PSA change quantity before and after antibiotic treatment can help to diagnose prostate cancer.

Objective To explore the effect of hepatocyte growth factor/scatter factor (HGF/SF) on apoptosis of colorectal cancer cells induced with curcumin. Methods MTT assay was used to evaluate the cytotoxicity of curcumin to colorectal cancer cells. Flow cytometry was used to detect the anti-apoptosis effect of HGF. Results Flow cytometry showed only 64 ?g/ml curcumin could play the proliferation-inhibiting role in Caco-2 cells leading to their apoptosis; at the same time, different concentrations of HGF could antagonize this inhibitory effect resulting in the decrease of apoptosis, but HGF worked without a concentration-dependent manner. The study on MAPK pathway showed that the protective effect of HGF on the apoptosis of Caco-2 cells was not influenced by inhibiting p42/p44 MAPK and p38 MAPK pathway. Conclusion HGF/SF antagonizes the apoptosis of Caco-2 cells induced with curcumin, but MAPK signaling pathway might not participate in this process.

OBJECTIVE To know the use of antibiotics in fever clinic of our hospital,and analyze whether it is reasonable.METHODS The situation was reviewed and analyzed that antibiotics were used for patients in fever clinic during the 4th quarter of 2005 by the doctor registry,original prescriptions,and data records of laboratory.RESULTS There were some unreadable aspects in the choice of antibiotics,the using method,drug combination etc in fever clinic of our hospital.CONCLUSIONS Fever clinic should enhance supervision management of the proper use of antibiotics.

OBJECTIVE To determine the pathogenic distribution and antimicrobial resistance of community acquired bacteremia in 105 children patients.METHODS Clinical profile,results of blood culture and antibiotic susceptibility test of 105 patients with community-acquired bacteremia treated from Jan 2003 to Dec 2006 were studied retrospectively.RESULTS The Gram-positive bacteria were from 58 cases which accounted for 55.2%.Most of them were Staphylococcus epidermidis,S.haemolyticus and S.hominis.The positive rate was 19.0%,15.5% and 10.3%,respectively.The Gram-negative bacilli were seen from 47 cases which accounted for 44.8%.Escherichia coli,Stenotrophomonas maltophilia and Klebsiella pneumoniae were the main microrganism in Gram-negative bacilli,the positive rate was 19.1%,14.9% and 10.6%,respectively.The resistant rate of Staphylococcus to penicillin was 100%.Their resistant rate to erythromycin was 94.7%.100% Gram-positive bacteria were susceptible to vancomycin,most of them were susceptible to ciprofloxacin,rifampicin and nitrofurantoin.The resistant rate of Gram-negative bacilli to ampicillin was 91.7%.Most of Gram-negative bacilli were susceptible to the third generation cephlosporin plus enzyme inhibitor,ciprofloxacin and imipenem.Resistance of the isolated pathogens to several commonly used antibiotics in pediatrics was observed.CONCLUSIONS We must focus on children aged under 3 years who are at risk of developing bacteremia.Empirical treatment with antibiotics is recommended only in life-threatening sepsis cases in pediatric emergency department.

Objective To study the chemical constituents and structures of the glycosaminogly-cans isolated from the leftover bits of the Chlamys farreri and Argopecten irradisus. Methods The qualitative and quantitative analyses of monosaccharides obtained by alcoholyses with HCl-methylalcohol from the samples were done by gas chromatography with standard contrast and inner standard methods. Mixed with KBr and pressed into pellet, the samples were analyzed by infrared spectrometer scanning from 4000-500 cm-1. Results The contents of monosaccharides from the samples were rhamnose 0. 75%, xylose 0. 63%, fucose 0. 67%, mannose 0. 97%, glucose 1. 11% and galactose 1. 59%, respectively. The infrared spectrum showed that the samples had typical infrared spectra of glycosaminoglycan. Conclusions The glycosaminoglycan isolated from the leftover bits of the Chlamys farreri contains neutral monosaccharides. Compared with standard glycosaminoglycans, the infrared spectra of the two samples are similar to that of hyaluronic acid.