Objective To explore the reversion of astragaloside and NMDA receptor antagonist against the hyperphosphorylation of protein Tau induced by fetal intrauterine distress in neonatal rats.Methods The analysis of variance of factorial design was setted up two intervention factors which were fetal intrauterine distress (two levels:no disposition;a course of fetal intrauterine distress)and the drugs (three levels:iv Saline;iv astragaloside;iv MK -801).When the neonatal rats grew to 12weeks,the hippocampus was removed from the neonatal rats.Detected the content of glutamate in the hippocampus of rats by high performance liquid chromatography.The expression of protein Tau which includes p -AT8Ser202 and GSK -3β1H8 in the hippocampus of rats were detected by the methods of immunohistochemistry staining.Results Fetal intrauterine distress could significantly up -regulate the content of glutamate,which was not affected by MK -801,in the hippocampus of neonatal rats which was reduces by the astragaloside (P <0.05).And both influences presented subtracting effects (P <0.05).Fetal intrauterine distress and the drugs do not affect the total protein Tau in the hippocampus of rats (P <0.05).Fetal intrauterine distress could up -regulate the hyperphosphorylation of protein Tau in the hippocampus of neonatal rats which could be reduced by astragaloside and MK -801 (P <0.05).And the influences between fetal intrauterine distress and the drugs presented subtracting effects (P <0.05 ).Conclusion Our results indicate that fetal intrauterine distress reduce the hyperphosphorylation of protein Tau in neonatal rats though up -regulating the content of glutamate.GSK-3βis the key protein in this signaling pathway.

Objective: To approach a better solution for enhancing the therapeutic results of acupuncture therapy in the treatment of Tourette syndrome, by observing the clinical results of combined scalp with body acupuncture and mono-body acupuncture. Methods: Fifty-seven patients were randomized into a treatment group (31 cases) and a control group (26 cases). The patients in the treatment group all received combined scalp-body acupuncture treatment, while the patients in the control group were given mono-body acupuncture treatment, for 1 month as a treatment session. At the end of the third treatment session, the Yale Global Tic Severity Scale (YGTSS) would be compared between pre- and post-treatment. Results: In the treatment group, 2 patients were clinically cured, 4 showed markedly effective, 18 showed effective, and 7 failed, making a total therapeutic rate of 77.4%. In the control group, 0 were clinically cured, 3 showed markedly effective, 9 showed effective, 14 failed, making a total therapeutic rate of 46.2%. There was a significant difference between the two total therapeutic rates (P<0.05). Conclusion: The combination of scalp and body acupuncture had a better therapeutic result than the mono-body acupuncture therapy in the treatment Fifty-of Tourette syndrome.

OBJECTIVE:To investigate the clinical etiology,serum type and drug resistance of lung cancer complicated with pulmonary Haemophilus influenzae (Hi) infection,in order to provide reference for infection prevention and rational drug use. METHODS:Clinical data of 8025 inpatients with lung cancer complicated with pulmonary infection in our hospital from Jan. 2009 to Jun. 2016 were collected,and the clinical etiology of pulmonary Hi infection was analyzed. The slide agglutination method was used for serotyping,nitrocefin slip method was used to detect β-lactamase,K-B method was used for drug sensitivity test,WHO-NET 5.6 software was used to deal with the results of drug sensitivity test. RESULTS:Eleven factors as age,clinical classifica-tion,invasive operation,surgery,radiotherapy and chemotherapy,hospitalization time,use of broad-spectrum antibiotics or hor-mones and other were closely related to pulmonary Hi infection in lung cancer patients(P<0.05 or P<0.01). Among 8025 speci-mens,104 strains of Hi were detected with detection rate of 1.30%. Serum type NTHi accounted for 44.23%. Separable strains were mainly b type(22.12%),followed by f type(17.31%)and a type(11.54%). Among 104 strains of Hi,56 strains of β-lac-tamase were detected with enzyme-producing rate of 53.85%. Drug resistance of 104 strains of Hi to ampicillin,amoxicillin,com-pound sulfamethoxazole and chloramphenicol were all higher than 35%;drug resistance of Hi toβ-lactamase inhibitors,cephalospo-rins,carbapenems,azithromycin and other antibiotics were all lower than 20%. Drug resistance of β-lactamase producing stains to ampicillin,amoxicillin,compound sulfamethoxazole and chloramphenicol were all higher than those of non-producing strains,with statistical significance (P<0.01). There was no statistical significance in drug resistance to other antibiotics between producing stains and non-producing strains(P>0.05). CONCLUSIONS:The clinical etiology of pulmonary Hi infection in patients with lung cancer is complicated. The serum type of the isolate is mainly NTHi;enzyme production situation is not optimistic,but Hi keeps a low rate of drug resistance to most antibiotics. β-lactamase inhibitors,cephalosporins,carbapenems and azithromycin are first choice for the treatment of Hi infections.

Hazardous Substances ; Risk Assessment ; methods ; Workplace

Objective To investigate the protective effect of isoflurane preconditioning on myocardium against isehemia-reperfusion(I/R)injury and the possible mechanism.Methods Fifty male SD rats weighing 250- 300 g were randomly divided into 5 groups(n=10 each):Ⅰ control group(C);Ⅱ I/R group and 3 isoflurane preconditioning groups with 0.5%(Ⅲ),or 1.0%(Ⅳ)or 2.0% isoflurane(Ⅴ).The animals were anesthetized with intraperitoneal pentobarbital 40 mg?kg~(-1).The hearts were immediately excised and placed in cold K-H solution.The aorta was canuulated and heart retrogradely perfused with K-H solution aerated with 95% O_2 and 5% CO_2 at 37℃ and 10 kPa in a langendorff apparatus.Left ventricular end-diastollc pressure(LVEDP)and left ventrieular systolic pressure(LVSP)were measured from a fluid-filled latex balloon in the left ventricle.The isolated hearts were made globally ischemic for 30 min followed by 60 rain reperfusion in group Ⅱ-Ⅴ.In the 3 isoflurane preconditioning groups the hearts were perfused with K-H solution saturated with 0.5% or 1.0% or 2.0% isoflurane for 15 rain followed by 15 rain washout before ischemia.The cardiac function variables including LVEDP,LVSP dp/dt_(min),dp/dt_(max) and HR were measured after epuilibrium(baseline values),immediately before ischemia,at the end of 30 min ischemia and 60 min reperfusion.The infarct size and cytochrome C level in cytoplasm and mitochondria of myocytes were measured.Results I/R significantly increased LVEDP and decreased LVSP,dp/dt_(min),dp/dt_(max) as compare with control group.Sevoflurane preconditioning significantly attenuated the depression of cardiac function caused by I/R.Only LVEDP was significantly higher during reperfusion period in the 3 sevoflurane preconditioning group than in the control group but there was no significant difference in LVSP,dp/ dt_(min),dp/dt_(max) between control group and the 3 preconditioning groups.The infarct size was significantly smaller in the 3 preconditioning groups than in I/R group.Cytochrome C level was significantly increased in cytoplasm but decreased in mitochondria in I/R group as compared with control group.Sevoflurane preconditioning significantly ameliorated the release of cytochrome C from mitochondria to cytoplasm in the 3 sevoflurane preconditioning group.Conclusion Isoflurane preconditioning can protect the heart against I/R injury by attenuation of the release of cytochrone C from mitochondria to cytoplasm.

Objective To investigate the protective effects of propofol preconditioning on myocardium against hypothermia ischemia normothermia reperfusion injury on isolated rat hearts. Methods The Langendorff apparatus was used.Sixty SD rat hearts were divided randomly into 5 groups after 20-minute equilibrium(n=12): control(Con) group,hearts were continually perfused with K-H buffer for 175 min;ischemia/reperfusion(I/R) group,hearts were perfused with K-H buffer for 40 min,then subjected to global ischemia at 27 ℃ for 75 min,and followed reperfusion at 37 ℃ for 60 min;propofol preconditioning group 1(P1),group 2(P2),and group 3(P3),hearts were perfused with K-H buffer including 50,100,and 150 ?mol/L propofol for 10 min and followed reperfusion like I/R group,respectively.Heart rate(HR),left ventricular end-diastolic pressure(LVEDP), left ventricular developed pressure(LVDP) and ?dp/dtmax at the end of equilibration,pre-ischemia and at the end of reperfusion were recorded.The contents of creatine kinase(CK) and lactate dehydrogenase(LDH) in coronary effluent were measured at the end of equilibration and 1,10,20,30,and 60 min during reperfusion.The activity of superoxide dismutase(SOD) and the contents of maleic dialdehyde(MDA) were measured at the end of reperfusion.The area of infarct region was determined at the end of reperfusion. Results HR,LVDP,?dp/dtmax and SOD activity in P2 and P3 group were higher than those in I/R group(P

Objective:To explore the clinical effect of intravenous immunoglobulin combined with conventional therapy in the treatment of severe pneumonia in infants and children.Methods:Selected the January 2015 to April 2016 in our hospital for treatment of 88 cases of children with severe pneumonia were retrospectively analyzed,which 47 cases by routine treatment plus intravenous immunoglobulin protein (treatment group),41 cases of children only take routine therapy (control group),with a course of 2 weeks.Results:The symptoms of fever,dyspnea,cough,wheezing,pulmonary rales were improved faster in the treatment group than the control group (P＜0.05);after 2 weeks of treatment,IgG levels were significantly higher in the treatment group than the control group(P＜0.05);after 2 weeks of treatment,the serum IL-6 and TNF-α levels were significantly lower in the treatment group than the control group(P＜ 0.05);after 1 week of treatment,57.45％ of patients was cured in the treatment groups while only 34.15％ patients in the control group (P＜0.05).Conclusion:Intravenous immunoglobulin combined with conventional therapy in the treatment of infants with severe pneumonia can significantly improve the theraputic effect and enhance blood immunoglobulin levels.

Objective To assess the clinical efficacy of TIPS in the treatment of patients with refractory ascites and investigate the clinical factors associated with TIPS. Methods 21 consecutive patients with refractory ascites, 16 men and 5 women with mean age of 45 years (range 22-69 years) were followed up for an average of 337 days (range 50-1323 days). Asites/ abdomen circumference, serum parameters, stents function, time of followed up and survival rate were analized. Results The ascites was significantly reduced as compared with basal values (P

Objective To assess the clinical value of pre-operative embolization for nasopharyngeal angiofibroma. Methods 13 patients with nasopharyngeal angiofibroma confirmed by surgery and biopsy were retrospectively analysed. Bilateral carotid artery angiograpy was perfomed for demonstration of the arterial supply of tumor. According to the size and different caliber of arteries, embolization were seperately undertaken by different types of coil via 4.1or 5F Head-Hunter catheter. The embolization efficacy, embolization was evaluated by amount of blood loss. Results After coil embolization , no complication happened ranging 1～4 days (mean 2 days) in all 13 patients and then all the tumor masses were totally resected with mean blood loss of (584.6?379.4) ml (range 250～1500 ml). Conclusions Preoperative coils embolization is safe, reliable with less complication especially for reducing intraoperative blood loss and promoting the prognosis.

Objective To investigate the expression of DNA methyltransferase 1 (DNMT1)mRNA in tissue of human transitional cell carcinoma of bladder(TCCB)and its clinical significance.Methods DNMT1 mRNA expression in 35 tumor tissues and 10 normal bladder tissues was measured by real-time PCR.Results Higher expression of DNMT1 transcripts was detected in tumor(3.25±0.74)than in normal tissues(1.53±0.44,P＜0.001).The expression level of DNMT1 mRNA in stage Tis-T1,T2-T4 was 3.14±0.67,3.31±0.84;in grade Ⅰ,Ⅱ,Ⅲ was 3.13±0.59,3.25±0.64,3.43±0.55;in patients older tham 40 years and younger than 40 years was 3.34±0.50.3.01±0.27;in male and female group was 3.31±0.42,3.01±0.20;in primary and recurrence group was 3.21±0.63,3.45±0.33.mRNA levels did not correlate with grade.stage and recurrence (P＞0.05).Higher levels of expression were associated with advanced age(P＜0.05).Male group had significant higher levels of expression than female group(P＜0.05).Conclusions Over-expres-sion of DNMT1 may play an important role in TCCB.DNMT1 can be an important therapeutic target in TCCB.