Objective To evaluate the clinical effectiveness of oral care combining rhEGF on curing oral ulcer caused by nasopharyngeal carcinoma radiotherapy.Methods 48 patients with oral ulcer caused by radiotherapy were divided in two groups randomly,rhEGF was sprayed on ulcer after oral care in the experimental group.The control group was transacted oral care as usual.The curative effect of two groups were observed.Results The results showed that the effective rate was 67 % and 92% in the control group and the experimental group respectively, which was statistically significant.Conclusion Oral care combining rhEGF may cure oral ulcer caused by radiother- apy,and it is worth using widely.

BACKGRoUND:Endothelial basal medium is mainly used to culture endothelial progenitor cells.Studies on mesenchymal stem cells (MSCs) cultured in this medium are few. OBJECTIVE:To compare the outcome of MSCs cultured in different mediums including endothelial basal medium. DESiGN.TIME AND SETTING:The control eell experiment was performed at the Muniopal Key Laboratory of Xinhua Hospital of Shanghai.China from September 2005 to May 2006. MATERIALS:Eight pregnant mongrel dogs were selected to obtain umbilical cord blood for isolation and culture of stem cells. Endothelial eell basal medium and endothelial cell medium were bought from Clonetics.USA.Mouse anti-CDlla monoclonal andbody,mouse anti-CDllb monoclonal antibody.mouse anti-CD29 monoclenal antibody and mouse anti-CD7l monoclonal antibody Were purchased from Antibody diagnostica,USA.Mouse anti-CD34 monoclonal antibody was obtained from Lab Vision Corporation.USA.METHODS:Umbilical cord blood stem cells were divided into four groups.Umbilical cord blood steTn cells in the group A were incubated in the endothellal basal medium.Umbilical cord blood steHl cells in the group B were incubated in the endothelial basal medium containing microvascular endothelial cells in a 6-well plate.Umbilical cord blood stem cells in the group C were incubated in the endothelial basal medium containing endotheliaI medium in a 6-well plate coated with fibronectin.Umbilical cord blood stelrn cells in the group D were incubated in the endothelial basal medium containing endothelial medium in a 25 cm2 ctdturing flask.MAIN OUTCOME MEASURES:Cell morphology and population doublings were observed.CDlla,CDllb,CD34,CD29 and CD71 expression was detected by immunohistochemistry. RESULTS:Fibroblast-like cells were measured in each group.The celIs grew badly in morphology and proliferated slowly in the group A,while cells proliferated rapidlyinthe group B.The cell clones were instable and inclined to aging in the group C,with a new cell clone was found.The cells in the group D were similar to those in the group C.Surface antigens detected by immunohistochemistry showed CDlla(-),CDllb(-),CD34(-),CD29(+)and CD7l(+). CONCLUSION:MSCs witll well growth and rapid proliferation can be detected in the endothelial basal medium containing endothellal medium in an intact 6-well plate.The outcome is bad in fibronectm-coated plate or 25 cm2 culture flask.

0.05),while we found significant difference in hospital duration between more than 60 days group and less than 30 days group(P

Transrectal ultrasonography is one of the early diagnostic approaches of prostate cancer.In this article,the recent study progression of conventional transrectal ultrasonography with combination of new techniques,such as contrast-enhanced ultrasonography,elastography,image fusion,and so on,in diagnosis of prostate cancer was summarized on the basis of a review of the literatures.

Objective: To prepare plumbagin transfersomal (PBG-T) gel and investigate its transdermal penetration characteristics in vitro. Methods: Plumbagin transfersomes were prepared by film-ultrasonic dispersion method. The optimal prescription condition of PBG-T was selected by central composite design and response surface method. The formula of PBG-T gel was optimized by orthogonal test. The Franz diffusion cell was used to investigate transdermal penetration characteristics of PBG-T gel in vitro. Results: The optimal prescription condition of transfersomes was determined as drug 10.0 mg, phospholipids 700.0 mg, Tween-80 91.5 mg, ultrasonication time 13 min. The optimal prescription condition of transfersomal gel was 1% carbomer 940 as gel matrix, and 5% glycerol as the humectant. According to the optimized prescription, the entrapment efficiency, the mean particle size, and Zeta potential of PBG-T were (79.88 ± 2.26)%, (125.64 ± 4.54) nm, and (-30.97 ± 1.13) mV. The cumulative penetration rate of PBG-T gel was 70.0% at 12 h. Conclusion: The optimal preparation technique is stable and feasible. Transfersomal gel features a sustained release in vitro, the transfersomal gel can increase penetration rate of plumbagin through the skin of rats.

Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Humans ; Mesenchymal Stromal Cells ; cytology ; Myocytes, Cardiac ; cytology

Objective:To study the clinical effect and the endocrine mechanism of acupuncture therapy on menopause syndrome.Method:There were 47 cases with menopause syndrome,which were divided into two groups,32 cases in the acupuncture group,treated by acupuncture with main acupoins of Shenshu (BL 23),Zusanli (ST 36) and Sanyinjiao (SP 6),and 15 cases in the control group,treated by orally taking the Oryzanol.The Kupperman index of the two groups was observed before and after treatment for evaluating the therapeutic effect.The changes of blood-serum F2,T,FSH,LH of the 25 cases in the acupuncture group were tested by radioimmunoassay before and after the treatment.Results:The acupuncture therapy can relieve the clinical symptoms of menopause syndrome,and its therapeutic effect was better than Oryzanol.After being treated by acupuncture therapy,the content of blood-serum E2 obviously increased,while the contents of LH and T declined,and the FSH also had a tendency to lower,but it was not statistically significant.Conclusion:The therapeutic effect of acupuncture therapy for menopause is confirmed.The acupuncture therapy can improve the function of hypophysial-gonadal axis of the patient with menopause syndrome,which may be the main mechanism of the acupuncture therapy for treating menopause syndrome.

Objective To explore the clinical significance of interstitial chemotherapy applied in breast cancer.Methods Sixty breast cancer patients with modified radical mastectomy were divided into treatment group (40 cases) and control group (20 cases) according to whether using fluorouracil implants.The control group was given conventional treatment.In treatment group,distilled water washed the wound after conventional treatment,and fluorouracil implants (0.4 g) was placed below local tumor,between pectoralis major and pectoralis minor,below 0.5 cm axillary vessel.The bone marrow suppression,liver and renal function,gastrointestinal reaction,skin flap necrosis,subcutaeous hydrops,local recurrence were observed in the 2 groups.Results There were no significant differences in the rates of bone marrow suppression,liver and renal function abnormal,skin flap necrosis,subcutaeous hydrops and gastrointestinal reaction between treatment group and control group (P ＞ 0.05).The rate of local recurrence in treatment group was significantly lower than that in control group [0 vs.20.0％ (4/20)],there was statistical difference (P ＜0.05).Conclusion Intraoperative application of fluorouracil implants is safe and effective in modified radical mastectomy of breast cancer.

This paper systematically stated the sexual moral problems of the repairment of ruptured hymen resulted form different reasons and the moral principles and requirements for the operator.

Objective To assess the efficacy of different types of thymectomy for the treatment of myasthenia gravis(MG).Methods A retrospective analysis on 269 cases of MG,who were treated in our hospital from 1991 to 2006 by transsternal thymectomy(January 1991 to May 2002,n=161),thoracoscopic thymectomy(February 2002 to July 2005,n=67),or video-assisted thoracoscopic extended thymectomy(VATET,February 2005 to November 2006,n=41).Results The mean operation time of the transsternal group was significantly shorter than that in the other groups [transsternal group vs thoracoscopic and VATET groups:(97.5?17.5)min vs(130.3?31.5)min(q=12.991,P