Adult ; Female ; Humans ; Sclerosis ; pathology ; physiopathology ; Spleen ; pathology ; Splenic Neoplasms ; pathology

Objective To investigate the effect of high serum thyroid stimulating hormone (TSH) on malignant transformation of thyroid tissue in Hashimoto's thyroiditis (HT).Methods The data of 569 patients pathologically confirmed HT admitted from Jan.2009 to Dec.2013 were retrospectively analyzed.According to with or without papillary thyroid carcinoma (PTC),patients were divided into HT group and HT complicated with PTC group.Age,gender,preoperative TSH,free thyroxine,free tri-iodothyronine,thyroidperoxidaseantibody (TPOAb),antithyroglobulinanfibody (TGAb),and tumor multifocality were investigated.Results Among the 569 patients,306 patients had HT without PTC and 263 had HT with PTC.The avaerage age of patients complicated with PTC was (45.52±10.58) years,younger than that of patients without PTC (51.63+9.50) years (P＜0.05).In HT patients with or without PTC,31％ (81/263) and 22.5％ (69/306) of patients had high TSH levels,which was significantly different (P＜0.05).Among HT patients complicated with PTC,tumor multifocality was identified in 32％(26/81) of patients with high level of TSH and in 29％(52/182) of patients with normal level of TSH.The difference had no statistical significance (P＞0.05).Conclusion High preoperative TSH level may stimulate malignant transformation of thyroid tissue in HT patients.

Objective To investigate the anti - stress activity of thymopentin ethyl ester. Methods Anti - stress activity was evaluated by heat stress model and chronic uncontrolled stress model. Heat stress model;of 60 KM mice were divided into six groups. Except the controlled group, the other mice were maintained in 42℃ for 1h. Chronic uncontrolled stress model:of 60 KM mice were divided into six groups. Except the controlled group, the other mice were given three different stimulations once a day for continuous 21 d. The controlled group and model group were injected saline 0.2ml, and the three test groups were respectively injected thympentin ethyl ester at 2mg/kg,0.2mg/kg, 0.02mg/kg subcutaneously. The positive controlled mice were given thymopentin 0.2mg/kg subcutaneously. At the end of the experiment, plasma corticosteroid, IL -2 and SOD were determined according to the kit instructions. Results The activities of thymopentin ethyl ester in suppressed corticosteroid up - regulation and the elevated plasma IL - 2 and SOD level were more significant than those of thymopentin(P

OBJECTIVETo establish a method for analyzing amorphous organic compound components of space Platycodon grandflorum rapidly.

METHODFTIR was applied for measuring and comparing P. grandflorum of comparison group,the ground group and the 4" generation of space group.

RESULTSeveral active components (platycodin, polysaccharide etc.) contents of space group are increased obviously.

CONCLUSIONThe major components and the structures remained inextenso,and the effictive component contents are enhanced in the space P. grandiflorum. FTIR is a fast method to analyze the changes of amorphous organic compound components of the space traditional Chinese medicines.

Mutagenesis ; physiology ; Platycodon ; chemistry ; Polysaccharides ; chemistry ; Saponins ; chemistry ; Space Flight ; Spectroscopy, Fourier Transform Infrared ; methods ; Weightlessness

The actions of four organic acids in Radix isatidis, a traditional Chinese medicinal (TCM) herb, on Escherichia coli, Staphylococcus aureus and Shigella dysenteriae growth were investigated by microcalorimetry. The four organic acids were syringic acid, 2-amino-benzoic acid, salicylic acid and benzoic acid. The power-time curves of Escherichia coli, Staphylococcus aureus and Shigella dysenteriae growth with and without organic acids were acquired, meanwhile the extent and duration of inhibitory effects on the metabolism were evaluated by growth rate constants (k1, k2), maximum heat-output[0] power (P(m)) and peak time (t(m)). The inhibitory activity varied with different drugs. The sequences of anti-microbial activity of the four organic acids on Escherichia coli, Staphylococcus aureus and Shigella dysenteriae were all: syringic acid > 2-amino-benzoic acid > salicylic acid > benzoic acid. And benzoic acid promoted the growth of Staphylococcus aureus and Shigella dysenteriae. This study provides a basis for the further study on Radix Isatidis.
Anti-Bacterial Agents ; isolation & purification ; pharmacology ; Benzoic Acid ; isolation & purification ; pharmacology ; Calorimetry ; methods ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Escherichia coli ; drug effects ; Gallic Acid ; analogs & derivatives ; isolation & purification ; pharmacology ; Microbial Sensitivity Tests ; Salicylic Acid ; isolation & purification ; pharmacology ; Shigella dysenteriae ; drug effects ; Staphylococcus aureus ; drug effects

Animals ; Antigen-Antibody Complex ; analysis ; DNA ; immunology ; DNA, Protozoan ; immunology ; Female ; In Situ Hybridization ; Lupus Nephritis ; etiology ; Mice ; Mice, Inbred BALB C ; Trypanosoma ; genetics

OBJECTIVETo analysis the clinical characteristics of 1000 Hz probe tone tympanometry obtained from normal neonates who passed the OAE screening. To calculate the normal range of the variances of the tympanometry, which may serve as the guide for newborn hearing screening and detect middle ear function in neonates.

METHODSOAE screening were performed to screen the hearing with GSI-70 Automated OAE. The 1000 Hz probe tone tympanograms were obtained from 650 neonates who passed the TEOAE screening in both ears and were on the normal physiological conditions after birth with GSI Tympstar Version II Middle Ear Analyzer. The means, the standard deviation, the 95% confidence interval were analyzed.

RESULTSThe 1000 Hz tympanometric data showed the 1Y1B1G tympanogram in 732 ears (56.3%), the 1Y3B1G tympanogram in 145 ears (11.2%), the 0Y0B0G tympanogram in 269 ears (20.7%), other shapes in 154 ears (11.8%) according to the Vanhuyse model. The 1000 Hz tympanometric datas showed the single-peaked tympanogram in 967 ears, the 95% confidence interval of the tympanometric data were as follows: tympanometric peak pressure (Tpp) was from -55.0 to 180.0 daPa, peak compensated static acoustic admittance (Peak Ytm) was 0.03-1.18 mmHo, tympanometric width (TW) was 70.0-230.0 daPa.

CONCLUSIONSThe majority of 1000 Hz tympanograms from this study show the single-peaked in normal neonates. The 95% confidence interval of the tympanometric data may serve as a guide for hearing screening and detecting middle ear function in neonates.

Acoustic Impedance Tests ; Female ; Hearing ; physiology ; Humans ; Infant, Newborn ; Male ; Neonatal Screening ; Reference Values

Objective:To construct a TPC-1 cell model that stably knocks out the HMGA2 by using CRISPR/Cas9 gene editing technology. Methods:Recombinant pLV[2gRNA]-EGFP:T2A:Puro- U6> {hHMGA2 [gRNA# A1]*}- U6>{hHMGA2 [gRNA#A2]*} of lentiviral plasmid vector was constructed: targeting HMGA2 Dual-gRNA sequence was designed, the synthesized Dual-gRNA fragment into pLV [2gRNA]-EGFP was cloned: T2A:Puro-U6 vector, extract a single clone for sequencing verification. the constructed recombinant plasmid vector with lentivirus was packed, and TPC-1 cells were infected, puromycin was used to obtain HMGA2 knock-out single clone, PCR and sequencing verification were performed, and real-time fluorescent quantitative qPCR was used to detect HMGA2 mRNA in cells Knockout efficiency. Results:After sequencing verification, pLV [2gRNA]-EGFP targeting HMGA2: T2A: Puro-U6>{hHMGA2 [gRNA#A1]*}-U6>{hHMGA2 [gRNA #A2]*} plasmid was successfully constructed; A single clone was picked for PCR identification and gene sequencing, TPC-1 cells were successfully obtained with HMGA2 gene completely knocked out; TPC-1 cells with HMGA2 knocked out were detected by real-time fluorescent quantitative qPCR, and they did not express HMGA2 mRNA.Conclusion:CRISPR/Cas9 gene editing technology enables us to construct a human papillary thyroid cancer cell line TPC-1 cell model with stable knockout of HMGA2.

Objective To investigate the relationship between apoptotic protein Bcl-2 and Bax in rat hippocampal neurons and cognitive dysfunction in rats with abnormal thyroid function.Methods Thirty healthy Wistar rats of 8-week-old SPF grade were randomly divided into three groups:(1) Normal control group (n=10);(2)hypothyroidism group (n=10);(3) hyperthyroidism group (n=10).All rats were sacrificed at the 4th week by heart blood sampling.The serum T3 and T4,TSH were measured.Morris water maze was used to train rats in each group for 6 days.At the end of the experiment,the hippocampus was taken from the rats,and HE staining was performed.The expression of apoptotic protein bcl-2 and Bax in rat hippocampal neurons was detected by immunohistochemistry.Results ①The escape latency of hyperthyroidism and hypothyroidism group was higher than that of the normal group at different time points (P＜0.05).In the test of the target area dwell time,the difference between the hyperthyroid group,the hypothyroid group and the normal group was statistically significant (P＜0.05).In the distance test of the target quadrant,The differences between the three groups were statistically significant (P＜0.05).In the number of passes through the target quadrant,the difference between hyperthyroidism group,hypothyroidism group and normal group was statistically significant (P＜0.05),but there is no significant difference between hyperthyroidism group and hypothyroid group (P＞0.05).②Hippocampus tissue HE staining:normal control group hippocampal neurons neatly arranged,the shape of the structure was complete and uniform,the nucleus was round or oval,nucleolus obvious,chromatin uniform level and more clear,nucleus round or oval,obviously,hyperthyroidism group,hypothyroidism group of neuronal structure loose,the number decreased,arranged disorder,deep nuclear staining,shrinkage,nucleolus disappeared,cytoplasm around the empty halo,cell spacing larger.③The positive cells expressing Bcl-2 and Bax protein in the hyperthyroidism group and the hypothyroidism group were increased compared with the normal control group (P＜0.05).Compareds with hyperthyroidism group,the expression of Bcl-2 positive cells was increased in hypothyroidism (P＜0.05).Conclusions ①The spatial learning and memory abilities of the rats with hypothyroidism and the hyperthyroid are lower than those in the normal control group.②The number of apoptotic protein positive cells in Bcl-2 and Bax neurons of hippocampus in rats with hypothyroidism and hyperthyroid increased,and the proportion of Bcl-2 and Bax was impaired,which indicates that apoptosis occurred in hippocampal neurons.This process may be one of the pathogenesis of cognitive impairment.

Objective To investigate the levels of serum Vitamin D(VD) from preconception to pregnancy in Jiading district of Shanghai, and explore the risk factors of VD concentration deficiency in the second and third trimester of pregnancy. Methods A total of 94 women who planned to have antenatal care and delivery in Maternal and Child Health Hospital of Jiading district of Shanghai from September 2016 to December 2018 were recruited as the study participants. Chemiluminescent immunoassay (CLIA) was used to examine the concentration of women’s serum VD from preconception to pregnancy. A total of 282 serum samples were detected. Results The prevalence of VD deficiency among 94 women from preconception to pregnancy were 40.4%,57.4% and 48.9% respectively. Results of the mixed linear model showed that women who had dyed or permed hair within the past 1 year had significantly lower serum VD levels during pregnancy (P<0.05), and women who often drank milk and ate deep-sea fish during pregnancy had higher VD levels during pregnancy (P<0.05). Conclusions VD deficiency was common among women in Jiading district of Shanghai, and it should be emphasized to supplement VD before and during pregnancy.