Objective: To investigate the feasibility and operative effect of the one-stage microsurgery for clipping of bilateral posterior communicating artery aneurysms (BpcoAA). Methods: The clinical data of 28 patients with BpcoAA were analyzed retrospectively. All patients underwent craniotomy and microsurgical clipping of BPcoAA via unilateral pterional approach. The patients were followed up for 6 months to 3 years after microsurgery. The head 3 D-CTA of the patients and their general conditions were reexamined. The Glasgow outcome scale (COS) scores were used to assess the prognosis. Results: Circled digit oneGood preoperative 3D-CTA showed 28 cases with 56 aneurysms, the aneurysms were clipped completely in 24 cases, the contralateral aneurysms were not clipped completely in 3 cases, the aneurysm was not clipped completely on the approach side in 1 case, and none of the patients died. The aneurysms ruptured in 8 cases on the approach sides during the microsurgery. Circled digit twoAfter microsurgery, 7 cases had hydrocephalus, and 5 had vasospasm on the approach sides and 3 on the contralateral sides, 3 suffered pulmonary infection, and 2 had oculomotor nerve injury on the contralateral sides. Circled digit threeThe mean follow-up time of the patients was 1.7 years. The GOS scores: 5 points in 10 cases, 4 points in 7 cases, 3 points in 9 cases, and 2 points in 2 cases. There were no aneurysm recurrence and new aneurysm formation. The patients whose aneurysms were not clipped completely had no recurrence of bleeding. Conclusion: According to 3D-CTA examination, using unilateral pterional approach, aiming at the location of the optic chiasm and the contralateral aneurysm pointing, the one-stage microsurgery for clipping of BpcoAA is safe and feasible.

Aged ; Antibodies, Monoclonal, Murine-Derived ; therapeutic use ; Antigens, CD20 ; metabolism ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cyclophosphamide ; therapeutic use ; Doxorubicin ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Lymphoma, Large B-Cell, Diffuse ; drug therapy ; metabolism ; pathology ; surgery ; Neoplasm Invasiveness ; Prednisone ; therapeutic use ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Proto-Oncogene Proteins c-bcl-6 ; metabolism ; Rituximab ; Uterine Neoplasms ; drug therapy ; metabolism ; pathology ; surgery ; Vincristine ; therapeutic use

Objective To discuss the relationship between the diameter of portal vein and esophagogastric variceal bleeding and the severity of liver cirrhosis by CT portal venography ( CTPV). To analyze the occurence about esophagogastric variceal bleeding under in different liver cirrhosis degree. Methods 60 patients of portal hyperten-sion with liver cirrhosis and 15 healthy volunteers (controls). According to Child-Pugh classification, 60 patients were divided into Child-Pugh A,B and C groups,According to the patients whether the esophagogastric variceal bleeding or not, it was divided into two groups that esophagogastric variceal bleeding (EVB) and no EVB. All of patients underwent with 64-slice spiral CT. Image post-processing techniques such as MIP, VR, MPR and SSD were applied to measuring the diameters of portal venous system vessels and depict the portosystemic collaterals of portal venous system. Results The diameters of the right branch of portal vein and super mesenteric vein were no statisti-cal significance between bleeding group and no bleeding group. The rest parameters of portal system in EVB group are all larger than those of no EVB group(P<0.05). Age and gender in two groups had no statistic significance. All diameters of portal system in cirrhotic group were all larger than those of control group(P<0.05). In different liver function,there are differences in each groups of diameter. The bleeding rate of different groups according to he-patic function showed statistical significance(P <0.05), higher the degree of liver cirrhosis, higher the bleeding rate. Conclusion The diameters of portal system in EVB group are larger than no EVB. All diameters of portal sys-tem in cirrhotic group are all larger than those of control group. There is difference the diameter of vascular in differ-ent hepatic function. Different degree of liver cirrhosis can predict the esophagogastric variceal bleeding.

Objective To observe the effects of over expression and inhibition expression of SAA protein on biological behavior of nasopharyngeal carcinoma CNE2 cells.Methods pcDNA3.1 (+)-SAA-CNE2 cell lines of high expression and pGPU6/GFP/Neo-SAA-CNE2 cell lines of interference expression of SAA protein in vitro.These two cells constructed by transfection of pcD-NA3.1(+)-SAA plasmid of SAA high expression and pGPU6/GFP/Neo-SAA plasmids of SAA inhibition expression respectively, plasmids of which were previously successfully reconstructed by the research group.Cell cycle of these two cells was analyzed by flow cytometry with PI staining.The ability of cell proliferation was inspected by plate cloning-forming test.Results Flow cytome-try showed that with the increase of expression of SAA protein,it had effect on promoting CNE2 cell division.Plate cloning-forming test showed that SAA protein can improve proliferation of the CNE2 cells.Conclusion SAA protein has the effect on promoting proliferation of human nasopharyngeal carcinoma CEN2 cell and migration in vitro.

A method has been developed for the determination of stimulants in products of nutritional supplement. The stimulants in the samples were extracted with a solution of sodium hydroxide and then cleaned up by liquid -liquid partitioning.The extracted stimulants were analyzed by gas chromatography with HP-5capilary column and detected by the Nitrogen-Phosphorus detector (NPD). The results showed that the method had good purifying effect with high sensitivity. The operation was also simple and quick. The detection limit ranged from 5 to 40 ?g/L and the average recovery rate was 75.4%～143.1% with relative standard deviations (RSDs) ranged from 0.27% to 5.5%.

0.05).Conclusion The incidence of VVC is higher in pregnant women with GDM than in pregnant women with GIGT,however, GCT,and OGTT show no statistical differences between women with VVC and those without VVC.

Purpose To summarize the clinical and echocardiographic characteristics of congenital quadricuspid aortic valves (QAV) so as to improve the understanding of QAV and the accuracy of preoperative diagnosis.Materials and Methods The clinical and echocardiographic data of 7 patients with QAV at the First Affiliated Hospital of Nanchang University were retrospectively studied,and the features such as the aortic valve (AV) leaf number,echo,shape,opening and closing movements,and its hemodynamics were observed and compared with surgical follow-ups.Results Five out of the 7 patients had chest tightness,chest pain,fluster,shortness of breath,and 2 others had no discomfort.The echocardiography presented that all the cases had moderate or severe aortic valve regurgitation.4 patients were diagnosed as QAV,2 patients were not diagnosed definitely,and the rest 1 was misdiagnosed.6 patients underwent aortic valve replacement and were all confirmed with QAV,among whom 2 patients were combined with infective endocarditis,and 1 patient was with aortic dilatation.All the surgical operations were successful and the patient's physical conditions were good after surgery.Conclusion Echocardiography plays an important role in diagnosis of aortic valve,but it is possible to be missed or misdiagnosed.Most QAV patients have good prognosis,but close follow-ups are needed when QAV is combined with other complex deformity or has induced secondary damages.

Adaptor protein ClpS is an essential regulator of prokaryotic ATP-dependent protease ClpAP, which delivers certain protein substrates with specific amino acid sequences to ClpAP for degradation. However, ClpS also functions as the inhibitor of the ClpAP-mediated protein degradation for other proteins. Here, we constructed the clpS-overexpression Mycobacterium smegmatis strain, and showed for the first time that overexpression of ClpS increased the resistance of M. smegmatis to rifampicin that is one of most widely used antibiotic drugs in treatment of tuberculosis. Using quantitative proteomic technology, we systematically analyzed effects of ClpS overexpression on changes in M. smegmatis proteome, and proposed that the increased rifampicin resistance was caused by ClpS-regulated drug sedimentation and drug metabolism. Our results indicate that the changes in degradation related proteins enhanced drug resistance and quantitative proteomic analysis is an important tool for understanding molecular mechanisms responsible for bacteria drug resistance.
ATP-Dependent Proteases ; metabolism ; Drug Resistance, Bacterial ; Endopeptidase Clp ; metabolism ; Mycobacterium smegmatis ; drug effects ; metabolism ; Proteolysis ; Proteomics ; Rifampin ; pharmacology

Objective To investigate clinicopathological characteristics of recurrent endometriosis and outcomes of secondary surgery.Methods From Jan.2003 to Dec.2008,69 cases with recurrent endometriosis operated by the same senior gynecologist in Peking Union Medical College Hospital were studied retrospectively in order to summarize clinicopathological characteristics and clinical outcomes.In prior surgery,both ovaries were involved in 29 cases (42％,29/69),and unilateral ovarian endometriomas were found in 40 cases ( 58％,40/69),including 19 cases (48％,19/40) with left lesions and 21 cases (52％,21/40) with right lesions.After first surgery,57 cases presented recurrent pelvic cystic masses and 12 cases presented moderate to severe pain without pelvic mass.Results The median recurrence interval was 38 ( 1 - 144) months.Among 57 cases with recurrent pelvic masses,bilateral ovarian endometiomas recurred in 24 cases at median recurrence interval of 31 months.Unilateral ovarian endometrioma recurred in 33 cases at recurrence interval of 39 months.There was no significant different recurrence period between blilateral and unilateral recurrent ovarian mass ( P =0.452).The recurrent rate of left and right side ovarian lesion was 77％ (37/48) and 68％ (34/50 cases),respectively,which did not reach statistical difference (P=0.396).Among 12 recurrent cases with pure pelvic pain,10 cases were founded combined with adenomyosis ( AM),of which 4 cases had deep infiltrated endometriosis (DIE).Compared with the 40 cases of simple ovarian endometriomas,29 cases complicated by DIE and (or) AM had longer operation time (75.1 min vs.49.9 min,P=0.017) and more blood loss (114.9 ml vs.38.4 ml,P＜0.05).In those 69 recurrent endometriosis patients,the median period of following-up was 32 months (3 months to 8 years).Six cases showed recurrent disease again at median recurrence interval of 3 years ( 6 months 6 years).Thirty-eight cases had expecting childbearing,however,only 4 women underwent childbirth.Conclusions The rate of clinical diagnosis of recurrent endometriosis is quite high.Pain is mostly associated with AM.The major difficulty and challenge of secondary surgery was ovarian endometrioma combined with DIE or AM.Prognosis of recurrent endometriosis is not optimistic after secondary surgery.

Objective To establish an LC-MS/MS method for determination of S-071031 B, a novel antidepressant , in rat plasma and to study its pharmacokinetic profiles .Methods An LC-MS/MS method was established to determine S-071031B in rat plasma, and L-8021 was employed as the internal standard .The analytes were separated on a C18 column with a mobile phase consisting of water-acetonitrile containing 0.1%(v/v) formic acid at a flow rate of 0.3 ml/min.The mass spectrometer was operated in a selected reaction monitoring ( SRM ) mode with a positive electrospray ionization (ESI) interface.The plasma concentration-time curve was drawn and pharmacokinetic parameters were calculated by DAS 2.0.Results The linear range was from 2 to 1000 ng/ml with a sensitivity of 2 ng/ml as the lower limit of quantification . The intra-day and inter-day precisions , recoveries and matrix effects at three spiked levels were all suited to the determina-tion of biological samples.After oral administration of S-071031B, the Cmax of S-071031B was (287.2 ±50.8) μg/L and the Tmax was (0.8 ±0.3) h, with a t1/2of (2.9 ±0.6) h and an AUC(0-∞)of (1372.6 ±255.3) μg/L· h.Conclusion This method is sensitive and specific enough for determination of S-071031 B in rat plasma to facilitate the study of its phar-macokinetics .