1.Antimicrobial effect of Malaysian green tea leaves (Camellia sinensis) on the skin microbiota
Hassanain Al-Talib ; Noor Alicezah Mohd Kasim ; Alyaa Al-Khateeb ; Chandrika Murugaiah ; Azrul Abdul Aziz ; Niena Nazleen Rashid ; Nazihah Azizan ; Shairah Ridzuan
Malaysian Journal of Microbiology 2016;12(6):423-427
Aims:
Camellia sinensis
(
green t
ea) is known for its therapeutic properties (anti
-
inflammatory, anti
-
oxidative and anti
-
ageing). The aim of this study was to determine the
in vitro
inhibitory activity of gree
n tea extract on some odorous skin
commensal bacteria.
Methodology and results:
Tea leaves were collected from
MARDI Agro Technology Park, Cameron Highlands. A
standardised protocol was used to obtain green tea extract. Aqueous green tea extracts were tes
ted for antibacterial
activity by well diffusion method. Minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)
assays were performed by broth microdilution assays using green tea extract concen
trations from 16 to 0.0313 mg/
mL
.
Green tea
extract showed antibacterial activity against skin microbiota. The high antimicrobial effect was achieved
against
Micrococcus luteus
with MIC and MBC of 0.125 and 0.25 mg/μL respectively, followed by
Staphylococcus
epidermidis
with MIC and MBC o
f 0.25 and 0.25 mg/μL respectively,
Bacillus subtilis
with MIC and MBC of 0.5 and 0.5
mg/μL respectively and lastly,
Corynebacterium xerosis
with MIC and MBC of 0.5 and 1.0 mg/μL respectively.
Conclusion, significance and impact of study:
The results obta
ined from the study confirm the
in vitro
anti
-
microbial
activity of green tea extracts against skin microbiota. The antibacterial effects of green tea against skin bacteria with its
anti
-
oxidant and anti
-
aging properties will help in keeping skin healthy,
fresh and reducing unpleasant
odo
rs
.
Camellia sinensis