1.Traumatic cerebrospinal fluid leakage following septorhinoplasty.
Ahmed YOUSSEF ; Shahzad AHMED ; Ahmed Aly IBRAHIM ; Mulvihill DANIEL ; Hisham M ABDELFATTAH ; Haitham MORSI
Archives of Plastic Surgery 2018;45(4):379-383
Septoplasty/septorhinoplasty is a common ear, nose and throat procedure offered for those patients with deviated septum who are suffering from nasal obstruction and functional or cosmetic problems. Although it is a basic and simple procedure, it could lead to catastrophic complications including major skull base injuries which result in cerebrospinal fluid (CSF) leaks. We describe two different cases of traumatic CSF leaks following septoplasty/septorhinoplasty at two different sites. The first patient suffered a CSF leak following septoplasty and presented to Alexandria University Hospital. The leak was still active at presentation and identified as coming from a defect in the roof of the sphenoid sinus and was repaired surgically. The second patient presented 4 days after her cosmetic septorhinoplasty with a CSF leak and significant pneumocephalus. She was managed conservatively. Understanding the anatomical variations of the paranasal sinuses and implementing proper surgical techniques are crucial in preventing intracranial complications when performing either septoplasty or septorhinoplasty. A good quality computed tomography of the nose and paranasal sinuses is a valuable investigation to avoid major complications especially CSF leaks following either procedure.
Cerebrospinal Fluid Leak*
;
Cerebrospinal Fluid Rhinorrhea
;
Cerebrospinal Fluid*
;
Diabetes Insipidus
;
Ear
;
Humans
;
Nasal Obstruction
;
Nose
;
Paranasal Sinuses
;
Pharynx
;
Pneumocephalus
;
Rhinoplasty
;
Skull Base
;
Sphenoid Sinus
2.Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.).
Abdul Qayyum RAO ; S Sarfraz HUSSAIN ; M Saqib SHAHZAD ; S Yassir Abbas BOKHARI ; M Hashim RAZA ; Allah RAKHA ; A MAJEED ; A Ali SHAHID ; Zafar SALEEM ; Tayyab HUSNAIN ; S RIAZUDDIN
Journal of Zhejiang University. Science. B 2006;7(4):291-298
Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.
2,4-Dichlorophenoxyacetic Acid
;
Benzyl Compounds
;
Cotyledon
;
growth & development
;
Culture Media
;
Gossypium
;
embryology
;
genetics
;
growth & development
;
metabolism
;
Hypocotyl
;
growth & development
;
Kinetin
;
Naphthaleneacetic Acids
;
Plant Growth Regulators
;
Purines
;
Regeneration
;
physiology
;
Transformation, Genetic
;
Zeatin
3.Seroprevalence and Spatial Distribution of Toxoplasmosis in Sheep and Goats in North-Eastern Region of Pakistan.
Haroon AHMED ; Ayesha MALIK ; Irfan MUSTAFA ; Muhammad ARSHAD ; Mobushir Riaz KHAN ; Sohail AFZAL ; Shahzad ALI ; M Mobeen HASHMI ; Sami SIMSEK
The Korean Journal of Parasitology 2016;54(4):439-446
Toxoplasmosis is a protozoan disease that is caused by Toxoplasma gondii in livestock and humans. Due to its medical and veterinary importance, it is essential to study the seroprevalence of T. gondii infection among humans and animals in various parts of the world. The major objective of this study was to determine the seroprevalence and spatial distribution of toxoplasmosis in small ruminants (sheep and goats) of north-eastern region, Pakistan. A total of 1,000 animals comprising of sheep (n=470) and goats (n=530) were examined for T. gondii infection by using ELISA. An epidemiological data was collected in the form of questionnaire. A surface has been generated by using method of interpolation in Arc GIS with the help of IDW (inverse distance weight). The results showed higher seroprevalence of T. gondii in goats (42.8%) as compared to sheep (26.2%). The seroprevalence was higher in females as compared to males in all examined ruminants. Similarly, there is a wide variation in the seroprevalence of T. gondii in different breeds of sheep and goats showing higher seroprevalence in Teddy (52.8%) and Damani breed (34.5%) of goat and sheep's, respectively. The geographical and spatial distribution of T. gondii shows that it is widely distributed in different parts of the north-eastern region of Pakistan. Our results suggest widespread environmental contamination with T. gondii oocysts. It suggests us that small ruminants could be a potentially important source of T. gondii infection if their infected meat is consumed undercooked.
Animals
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Goats*
;
Humans
;
Livestock
;
Male
;
Meat
;
Methods
;
Oocysts
;
Pakistan*
;
Ruminants
;
Seroepidemiologic Studies*
;
Sheep*
;
Toxoplasma
;
Toxoplasmosis*
4.Clinical utility of CA-125 in the management of uterine carcinosarcoma.
Koji MATSUO ; Malcolm S ROSS ; Mayu YUNOKAWA ; Marian S JOHNSON ; Hiroko MACHIDA ; Kohei OMATSU ; Merieme M KLOBOCISTA ; Dwight D IM ; Shinya SATOH ; Tsukasa BABA ; Yuji IKEDA ; Stephen H BUSH ; Kosei HASEGAWA ; Erin A BLAKE ; Munetaka TAKEKUMA ; Masako SHIDA ; Masato NISHIMURA ; Sosuke ADACHI ; Tanja PEJOVIC ; Satoshi TAKEUCHI ; Takuhei YOKOYAMA ; Yutaka UEDA ; Keita IWASAKI ; Takahito M MIYAKE ; Shiori YANAI ; Tadayoshi NAGANO ; Tadao TAKANO ; Mian MK SHAHZAD ; Frederick R UELAND ; Joseph L KELLEY ; Lynda D ROMAN
Journal of Gynecologic Oncology 2018;29(6):e88-
No abstract available.
Carcinosarcoma*
5.Antiviral activities of Cholistani plants against common poultry viruses
Shahzad, M.I. ; Anwar, S. ; Ashraf, H. ; Manzoor, A. ; Naseer, M. ; Rani, U. ; Aslam, Z. ; Saba, N. ; Kamran, Z. ; Ali, S. ; Aslam, J. ; Arshad, M.
Tropical Biomedicine 2020;37(No.4):1129-1140
Herbal medicines are becoming more popular and acceptable day by day due to
their effectiveness, limited side effects, and cost-effectiveness. Cholistani plants are reported
as a rich source of antibacterial, antifungal, antiprotozoal, antioxidant, and anticancer agents.
The current study has evaluated antiviral potential of selected Cholistani plants. The whole
plants were collected, ground and used in extract formation with n-hexane, ethyl acetate and
n-butanol. All the extracts were concentrated by using a rotary evaporator and concentrate
was finally dissolved in an appropriate vol of the same solvent. All of the extracts were tested
for their antiviral potential by using 9-11 days old chick embryonated eggs. Each extract was
tested against the Avian Influenza virus H9N2 strain (AIV), New Castle Disease virus Lasoota
strain (NDV), Infectious bronchitis virus (IBV) and an Infectious bursal disease virus (IBDV).
Hemagglutination test (HA) and Indirect Hemagglutination (IHA) tests were performed for
different viruses. The overall order of the antiviral potential of Cholistani plants against
viruses was NDV>IBV>IBDV>AIV. In terms of antiviral activity from extracts, the order of
activity was n-butanol>ethyl acetate>n-hexane. The medicinal plants Achyranthes aspera,
Neuroda procumbens, Panicum antidotale, Ochthochloa compressa and Suaeda fruticose
were very effective against all four poultry viruses through their extracts. The low IC50
values of these extracts confirm the high antiviral potential against these viruses. It is worth
to mention that Achyranthes aspera was found positive against IBDV through all its extracts
which overcome the problem of unavailability of any known drug against IBDV. In short, the
study proved that Cholistani plants are rich source of antiviral agent and their extracts can be
used as good source of antiviral drugs both in crude and in purified form.
6.Epidemiological investigation and risk factors of Peste des petitis ruminants (PPR) in yaks (Bos grunniens) and cattle in five regions of China
Li, X.H. ; Li, K. ; Zhang, H. ; Gan, P. ; Luo, H.Q ; Han, Z.Q ; Mehmood, K. ; Shahzad, M.
Tropical Biomedicine 2018;35(3):736-743
To investigate the prevalence of Peste des petitis ruminants in yaks and cattle in
poorly studied areas of China. A total of 1202 and 560 blood samples were collected from
yaks and cattle, respectively and processed using a commercial ELISA kit. Multivariable
logistic regression model was piloted to find the variables, potentially associated with exposure
of PPR infection in yaks and cattle. Results indicated that the overall prevalence of PPR in
yaks was 11.2%. A total 66 (11.8%) out of 560 were examined out to be positive to PPR in
cattle in Jiangxi province. According to conditional stepwise logistic regression, region, age
and gender were found to be the more influencing risk factors in yaks, while region and age
were found to be the potential risk factors in cattle. The current results reported the prevalence
and associated risk factors of PPR in bovine for the first time in China.