1.Experimental study of silicone membrane implanted beneath the scleral flap during filtering surgery in rabbits
Cheng, PEI ; Nai-Xue, SUN ; Zhen, HAN ; Sha-Sha, LÜ
International Eye Science 2008;8(3):445-447
·AIM: To investigate the efficacy and security of the silicone membrane implant through observing intraocular pressure, filtering blebs and histopathologic results.·METHODS; Forty rabbits were randomly divided into 4 groups. Each group consisted of 10 rabbits. Trabecu-lectomy and silicone membrane implantation were performed in one eye of each rabbit, while the other eye was only performed trabeculectomy as control. The change of intraocular pressure, filtering blebs were observed postoperatively. And each eye had undergone histopathologic examination.·RESULTS:The duration of low intraocular pressure and existence of filtering blebs in implanted eyes was longer than that in controlled eyes. Light microscopy revealed that patent drainage tract and biting site could be seen in silicone membrane implanted eye. The activity and regularity of fibroblast proliferation in implanted eyes and controlled eyes were similar. Excessive expression of fibroblast proliferation was not induced by silicone membrane.·CONCLUSION: Characterized by safety, effectiveness and simplicity, silicone membrane implantation can act as a new drainage surgery.KEYWORDS: silicone membrane; drainage surgery;intraocular pressure
3.Anti-inflammatory Effect of Fukang Granules
Liming HUANG ; Jianping SHA ; Chengrong SUN
China Pharmacy 1991;0(06):-
OBJECTIVE:To study the anti-inflammatory effect of Fukang granules (FK,prepared from traditional Chinese herbs,such as Radix Astragali,Radix Codonopsis,Angelica sinensis,etc.). METHODS:The anti-inflammatory action of FK was studied by carrying out rat paw edema test,mouse auricular swelling test,rat chronic pelvic inflammation test and rabbit salpingitis test.RESULTS:FK could lessen rat paw edema,mouse auricular swelling and rat pelvic inflammation and rabbit salpingitis,reduce blood viscosity and blood reduction viscosity and the coefficients of cell aggregation and thrombosis. CONCLUSION:FK has anti-inflammatory effect and the action of activating blood circulation to dissipate blood stasis.
4.Rapid detection of Escherichiacoli with test paper of Na p-nitrophenyl ?-D-Glucuronate
Jiali SUN ; Sha MI ; Xiaolan LIU
Chinese Journal of Laboratory Medicine 2001;0(03):-
Objective To develop a new method for rapid detection of E.Coli.with a new substrate Na p nitrophenyl ? D glucuronate (PNP Glucu Na). Method The test paper was immersed with of Na p nitrophenyl ? D glucuronate which was synthesized by us and another paper with culture medium and phorphate buffer.Colour change was observed to detect the E.coli. Results An E.coli concerntration of 4?10 7 took 0.5 hour, while a concentration of 4?10 0 took 12 hours. 138 strains of E.coli and 190 strains of other bacteria were examined . The sensitivity was 94.2% and the specificity was 92.6%. Conclusion This method is rapid, accurate, simple, and economical for detecting E.coli.
5.Risk factors of anti-tuberculosis drugs induced liver injury and its impact on the treatment outcome of patients treated with first line anti-tuberculosis drugs: a historic retrospective cohort study
Qin SUN ; Wenwen SUN ; Wei SHA ; Xuwei GUI ; Heping XIAO
Chinese Journal of Infectious Diseases 2015;33(12):725-730
Objective To explore the incidence and risk factors of anti-tuberculosis (TB) drugs induced liver injury (ATDILI) and to discuss its impact on the treatment outcome of patients treated with first line anti-TB drugs.Methods Among the patients who received anti-TB treatment with directly-observed treatment strategy (DOTS),121 patients with ATDILI and 817 patients without ATDILI were included in this retrospective cohort study.Binary Logistic regression model was used to analyze the risk factors of ATDILI in univariate and multivariate analysis.The x2 test was used to compare the treatment success rates and drug resistant rates.Kaplan-Meier analysis and Log-rank test were used to compare the sputum smear/culture conversion rates and cavity closure rates.Results The incidence of ATDILI was 12.9% (121/938) in this cohort.Multivariate Logistic regression showed that hepatitis B virus carrier with both hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) positive (OR=4.29,95%CI:2.15-8.58,P<0.01),complicated with systemic lupus erythematosus (OR=3.34,95%CI:1.46-7.63,P=0.004),serum albumin ≤25 g/L (OR=3.14,95%CI:1.50-6.58,P=0.002) and alcoholism (OR=1.79,95%CI:1.14-2.82,P=0.012) were independent risk factors of ATDILI.The treatment failure rate in patients with ATDILI was significantly higher than that in patients without ATDILI (19.1%[24/121] vs8.0%[65/817],OR=2.86,95%CI:1.71-4.78,P<0.01).The drug resistant rates of two groups were not significant different (4.1%[5/121] vs 1.7%[14/817],P>0.05).The sputum smear/culture conversion rate (85.4%[41/48] vs 94.0% [298/317],x2 =38.912,P<0.01) and cavity closure rate (84.6%[22/26] vs 93.0%[198/213],x2 =20.709,P<0.01) in patients with ATDILI were both significantly lower than those in patients without ATDILI.Conclusions The incidence of ATDILI is relatively high in hospitalized patients treated with first line anti-TB drugs.ATDILI has negative effects on treatment outcome of TB patient.Hepatitis B carrier with positive HBsAg and HBeAg,systemic lupus erythematosus,albumin ≤25 g/L and alcoholism may increase the risk of developing ATDILI.
7.Report of a child with transient hyperphosphatasemia of infancy and early childhood.
Qiu-sha PAN ; Yu SUN ; Ning-ning TAN
Chinese Journal of Pediatrics 2012;50(5):397-398
Alkaline Phosphatase
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blood
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Biomarkers
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blood
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Child, Preschool
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Female
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Humans
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Infant
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Isoenzymes
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blood
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Phosphorus Metabolism Disorders
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blood
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diagnosis
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Risk Factors
8.Cerebrospinal fluid adenosine deaminase activity for the diagnosis of tuberculous meningitis
Qin SUN ; Wei SHA ; Heping XIAO ; Qing TIAN ; Hong ZHU
Chinese Journal of Clinical Infectious Diseases 2010;03(5):263-266
Objective To evaluate cerebrospinal fluid adenosine deaminase(CSF-ADA)activity in the diagnosis of tuberculous meningitis(TMB), and to observe its dynamic changes. Methods A total of 160 patients were included and were divided into two groups: 76 cases of TBM and 84 cases of non-TBM.Among the cases of non-TBM, there were 36 cases of bacterial meningitis, 30 cases of viral meningitis and 18 cases of cryptocoocal meningitis. All the patients were measured with their CSF-ADA activity by Enzymecoupled assay(Trinder method)and 47 patients of TBM were measured again after 2 weeks' and 6 weeks'antitubercular therapy. Results were expressed as(-x)± s. Mann-Whitney U test and paired-samples t test were used. Results CSF-ADA activity in TBM group was(12.9 ±6.4)U/L, while that in the non-TBM group was(6.0 ± 4.1)U/L, the difference was of statistical significance(U = 7.860, P < 0.05). With the cutoff value of 9 U/L, the sensitivity and specificity to differentiate TBM from non-TBM was 84.21% and 83. 33%, respectively. CSF-ADA activity decreased in TBM patients after antitubercular treatment.Conclusions CSF-ADA activity can be an effective laboratory marker for early differential diagnosis of TMB with the cut-off value of 9 U/L. Dynamic changes of CSF-ADA activity may be a indicator for the effect of antitubercular treatment.
9.Immunohistochemical localization for nuclear factor kappa B p50 in cochlea of mice
Sun JIANHE ; Yang WEIYAN ; Sha SHUHUA ; Jochen SCHACHT
Medical Journal of Chinese People's Liberation Army 2007;32(1):1-4
Objective To show the capability of NF-κB expression in cochlea. Methods Kanamycin (KA) was subcutaneously injected twice daily for 3 and 7 days with an eight hours interval between two injections, and lipopolysaccharide (LPS) was injected into the tympanic cavity of mice. Equal amount of saline was injected for 7 days as control. Frozen sections of all mice cochleae were examined immmunohistochemichally with rabbit polyclonal NF-κB p50. Expression of NF-κB p50 immunoreactivity of mouse cochleae is identified as showed as brown reaction products characteristic of DAB immunohistochemistry. Results Immnoreactivity NF-κB p50 in mouse cochlea was localized in the organ of Corti, spiral limbus, tectorial membrane, the vascular stria, spiral ligament, spiral ganglion and nerve fibers. The immunoreaction could be observed in all spirals throughout the cochlea. Stronger staining was visible in spiral ligament, tectorial membrane, spiral prominence, spiral ganglion and nerve fibers, and the organ of Corti. The immunoreaction in the vascular stria was weaker than that in the structures mentioned above. The immunoreaction in the organ of Corti was observed in inner hair cells (IHC) and outer hair cells (OHC), inner and outer pillar cells, Deiter's cells, and Boettcher's cells. The immunoreaction was weaker in inner sulcus cells, Hensen's cells and Claudius'cells. The stronger immunoreaction was observed in nucleus of spiral ganglion cells. The nucleus of IHC and OHC remained unstained. Conclusion The injection of LPS/KA can promote NF-κB p50 expression to induce an acute reaction in mouse cochlea.
10.Clinical characteristics of children with rotavirus infection presenting with vomiting as main manifestation.
Yu SUN ; Qiu-Sha PAN ; Shi-Jun YAN
Chinese Journal of Contemporary Pediatrics 2009;11(1):74-75
Child, Preschool
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Female
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Humans
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Infant
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Male
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Rotavirus Infections
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diagnosis
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therapy
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Vomiting
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etiology