1.Comparing multiplex and multiplex real-time polymerase chain reaction with traditional blood culture in bacterial detection among patients with septicemia
Loan Thi Nguyen ; Linh Trong Nguyen ; Trang Thu Nguyen ; Hien Minh Nguyen ; Thuy Thi Pham ; Huyen Thi La
Malaysian Journal of Microbiology 2022;18(3):242-250
Aims:
This study was aimed to test the specificity of primers and probes with target genes by using multiplex PCR and multiplex real-time PCR methods. These methods were compared with traditional blood culture methods in detecting five bacteria causing sepsis, including Acinetorbacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus.
Methodology and results:
A total of 587 blood samples from patients diagnosed with sepsis and septic shock were collected at Thanh Nhan Hospital, Hanoi, Vietnam. Each sample was divided into three parts for bacterial culture, multiplex PCR and multiplex real-time PCR to detect the similarity of the two PCR methods with the bacterial culture method. Conditions in multiplex PCR and multiplex real-time PCR were optimized to ensure the successful amplification of target genes. Results showed that the primers and probes were tested completely specific to the target genes and using multiplex PCR and multiplex real-time PCR techniques could detect five pathogens causing sepsis, including A. baumannii, K. pneumoniae, P. aeruginosa, E. coli and S. aureus.
Conclusion, significance and impact of study
Both multiplex PCR and multiplex real-time PCR methods have high similarities with the culture method, showing potential in the application of bacteria detection in sepsis.
Multiplex Polymerase Chain Reaction
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Sepsis--microbiology
5.A case report of Wautersia sepsis infection.
Fang GAO ; Wen LU ; Wei-ping WEI
Journal of Southern Medical University 2011;31(3):559-560
Genus Wautersia is a Gram-negative bacterium that has rarely been associated with human infections abroad, and is firstly reported in home. We report a case of sepsis caused by Wautersia species, which was separated from blood and bone marrows.
Adult
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Cupriavidus
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isolation & purification
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Female
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Humans
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Sepsis
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microbiology
6.Fatal hemolysis due to clostridium perfrigens blood stream infection.
Bin CAO ; Ling-Ling SU ; Bin-Bin LI ; Ying-Mei LIU
Chinese Medical Journal 2013;126(18):3572-3573
7.Neonatal septicemia caused by Listeria monocytogenes: report of 6 cases.
Li WU ; Xian-hua ZHANG ; Hao CHEN ; Xue-lei YIN
Chinese Journal of Pediatrics 2008;46(1):22-25
OBJECTIVENeonatal listeriosis is a relatively rare but serious disease with a high mortality rate. This study was conducted to analyze the clinical features, treatment, and outcome of 6 cases with Listeria monocytogenes septicemia confirmed by positive blood cultures.
METHODSTotally 12,538 live births delivered in the hospital from January 1, 2004 to June 30, 2006 were investigated. Differences in the time of onset of the disease, clinical presentation, illness severity, laboratory data, management, and prognosis were compared between preterm and full-term infants.
RESULTSThe incidence of neonatal listeriosis was 4.8% in this study. All the cases with listeriosis were found to have early onset and the disease was transmitted from the mother to the fetus, 4 of the cases were delivered via cesarean section, 2 were born via normal spontaneous vaginal delivery. Maternal infection before parturition presented with fever in 4, diarrhea in 1; 5 had abnormal white blood cell counts and total neutrophil counts; 1 had positive result of Listeria monocytogenes in intrauterine contents culture. Three premature infants showed signs and symptoms of severe bacterial septicemia at birth, such as reduced activity, respiratory distress, poor skin color and poor peripheral perfusion; the enlarged liver was palpable 2 - 3 cm below the right costal margin and 5 cm below the xiphoid in one; congestive rashes over the body and muscular hypotonia. Abnormal results of laboratory tests included peripheral blood white cell count (21.6 - 33.8 x 10(9))/L, total neutrophil count 0.77 - 0.83; platelet count (102 - 59 x 10(9))/L; C-reactive protein (CRP) > (160 - 118) mg/L(24 - 72 h after birth). Three preterm infants who received intensive care, accompanied by pathological changes of lungs indicated by chest X ray required assisted mechanical ventilation and 2 of them survived without sequelae but the other one died at 51 h of life. The initial clinical signs of septicemia in 3 full-term infants appeared later than preterm newborn within 62 h, 63 h and 165 h, respectively after birth. They only had fever, T 38 - 39 degrees C, with lower activity in contrast to normal newborn and without other signs and symptoms of septicemia. Accurate diagnosis of listeriosis was established by positive bacterial blood cultures. Intravenous treatment of neonatal listeriosis with ampicillin or penicillins for 1 - 2 weeks was more effective, but cephalosporins were not effective.
CONCLUSIONSThere are sporadic cases with Listeria monocytogenes infection among pregnant women in this country, resulting in severe illness of their newborn infants. Early differential diagnosis, early detection of causative organisms, especially in newborn infants infected with Listeria monocytogenes, early treatment with sensitive antibiotics can decrease the mortality rate and improve neonatal outcome. It is necessary to enhance nationwide surveillance for listeriosis.
Female ; Humans ; Infant ; Infant, Newborn ; Infant, Newborn, Diseases ; diagnosis ; microbiology ; Listeria monocytogenes ; isolation & purification ; Listeriosis ; microbiology ; Male ; Sepsis ; diagnosis ; microbiology
8.Pasteurella multocida septicaemia following a dog bite.
; P K MAH ; S C CHUAH ; L S CHEW
Singapore medical journal 1993;34(3):271-273
Bite wounds are often mistakenly considered innocuous. However, they are frequently complicated by infection which may be serious. We describe a case of Pasteurella multocida septicaemia with myopericarditis following a dog bite. Treatment of the infection as well as active support of myocardial function led to a successful outcome.
Adult
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Animals
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Bites and Stings
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microbiology
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Dogs
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Hand Injuries
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microbiology
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Humans
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Male
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Pasteurella Infections
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pathology
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Pasteurella multocida
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Pericardial Effusion
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microbiology
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Pericarditis
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microbiology
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Sepsis
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pathology
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Wound Infection
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pathology
10.Intravenous drug abuse-related infective endocarditis: report of an autopsy case.
Wei-xiang ZHONG ; Dong-ping TIAN ; De-qing WU ; Min SU
Chinese Journal of Pathology 2010;39(6):421-422
Adult
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Aortic Valve
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microbiology
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pathology
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Autopsy
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Brain
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microbiology
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pathology
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Endocarditis, Bacterial
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complications
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microbiology
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pathology
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Female
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Heart Ventricles
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microbiology
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pathology
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Humans
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Mitral Valve
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pathology
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Sepsis
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complications
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microbiology
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pathology
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Substance Abuse, Intravenous
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complications
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microbiology
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pathology
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Young Adult