In order to clarify the physiological nature of the local differences in the skin electroconductivity we measured transcutaneous electric current on the representative (determinate) points of Ryodo-Raku (by Y. Nakatani) which correspond to so-called Genketu of traditional Chinese medicine with a wet electrode of 1cm in diameter in the condition of D. C. 12 volts and 200 uA when, both electrodes are directly connected. Eighty-two healthy adults were tested once and four other healthy adults were tested daily for eight days. We analysed mathematically the values of measurements by the principal component analysis.
As a result, we found that the electroconductivity of the skin in the upper and lower limbs both, and volar and dorsal sites of the upper limb changed independently each other and showed from rough to close interrelations according to the orders as above-mentioned.

We invented to clarify the influences of acupuncture on the regional skin electroconductivity on humans. An acupuncture needle in situ for a certain time or electroacupuncture using DC 12 volt, 200μA for 7 sec. was applied on the forearm.
Measurements of skin electroconductivity were done on several representative points which were distal or proximal to the acupuncture periodically.
A needle in situ and electroacupuncture on LI4 did not change the electroconductvity of the proximal skin area around the elbow level on the same extremity.
A needle in situ on LI11 increased electroconductivity of the regional skin on the distal area around Manus dorsalis.
Electroacupuncture on LI11 increased electroconductivity of the skin on LI5.
A needle in situ on LI7 did not change electroconductivity of the skin on the proximal elbow level, but significantly decreased electroconductivity of the skin on LU9.

We investigated changes of the amperage on the dorsalis by the hand acupuncture under the condition of DC 12 volt, 200uA when both electrodes are connected.
(Method) We leaved a stainless steel needle, 25mm lenght and 0.26mm in diameter, on the first lumbar and thigh region point of 16 healthy adults for 10min. 2×2cm aluminum foil coated with keratin cream was used as a elcectrode for the measurement. We measured in pre-practice, post-practice, 10min, 20min, and 30min. The measurement velues in every time were examined by means principal component analysis.
(Results) Acupuncture stimulation on the first lumbar and thigh region point showed the tendency of operation upon the symmetric L4resion.

According to the Ryodoraku autonomic nervous system therapy the average of the measurement values on Ryodo points (acupuncture point) which belong to the Ryodoraku (meridian) can be obtained from the measurement value on the representative point of the Ryodoraku.
They are judged relative excitement which corresponds to “excitement and suppression” (fullness and emptiness) in proportion as to the relative position on Ryodoraku chart.
We got the result that the knee joint pain relates to the excitement of H₂, the suppression of F₁, and the suppression of F₄.

It is necessary to research constituents of moxa on the studies of moxibution.
As we analyged the essential oil in moxa with gas liquid chromatography, n-nonacosane and n-hentriacontane were detected. Several compound (ex.: tricosanol, hentricontane, arachinakohol, thujone) have been already detected in moxa. But n-nonacosane and n-hentriacontane are newly detected constituents in moxa.