1.Bovine carriers of Anaplasma marginale and Anaplasma bovis in South India
Nair, A.S. ; Ravindran, R. ; Lakshmanan, B ; Sreekumar, C. ; Kumar, S.S ; Remya Raju ; Tresamol, P.V ; Vimalkumar, M.B ; Saseendranath, M.R.
Tropical Biomedicine 2013;30(1):105-112
Carriers of bovine anaplasmosis in Northern Kerala, South India were detected
using conventional microscopical and molecular techniques. PCR-RFLP and nested PCR
techniques were used for detection of Anaplasma marginale and Anaplasma bovis
respectively and the PCR products were confirmed by sequencing. Out of 150 samples
tested, 25 were detected positive for A. marginale and five for A. bovis based on molecular
tests. The inclusion bodies of A. marginale could be detected by microscopy in two blood
smears after staining by giemsa while acridine orange staining detected three smears positive.
The data clearly suggest the higher sensitivity of molecular techniques for diagnosis of these
diseases.
Result Analysis
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