Background: von Willebrand Diseases (vWD) is the most commonly inherited bleeding disorder. It is defined as a deficiency or abnormality of von Willebrand Factor (vWF) causing impaired hemostasis. Studies in a predominantly pediatric population reveal that the prevalence of vWD is 0.8-1.3 percent. Objective: To determine the prevalence of von Willebrand Diseases and to establish its clinico-hematologic profile. Methods: Ninety-nine patients with bleeding manifestations referred to the National Hemophilia Center from all over the Philippines were studied. Patients who fulfilled the inclusion criteria of at least 2 symptoms consistent with vWD, underwent initial screening tests: a complete blood count with actual platelet, blood typing, bleeding time, prothrombin time and activated partial thromboplastin time. Laboratory tests to diagnose vWD were done. Results: Thirty four patients (34.34 percent) with bleeding manifestations had vWD. Patients with vWD and those without were comparable as to age, sex distribution, family history of bleeding, blood type and bleeding manifestations. Among the patients with vWD, 11 (32.35 percent) had Type I vWD and 23 (67.65 percent) had Type 2 vWD. The mean FVIII, vWF: Ag and vWF: RCo were decreased. Conclusion: The study suggests that there is a high proportion of vWD among patients with bleeding tendency in the Philippines.
Human ; Male ; Female ; Adult ; Young Adult ; Adolescent ; Child ; VON WILLEBRAND DISEASES ; HEMORRHAGE

Objective: To evaluate antimicrobial and antioxidant properties of saline extract from Tithonia diversifolia leaves by phytochemical bioprospecting, and investigate its safety against animal cells. Methods: The saline extract was prepared, with NaCl (0.15 M), by constant stirring of the dried and pulverized leaves, followed by volume reduction by lyophilization. The extract was phytochemical characterized using ultra-performance liquid chromatography, and total phenol and flavonoid analysis also was performed. The antioxidant capacity was determined through DPPH· radical, the antimicrobial property was evaluated against standard bacteria and fungi, and the viability assays were performed against mice splenocytes. Results: Fifteen compounds were identified belonging to two main classes terpenoids and phenolics. The extract showed 22.185 mg GAE/g of total phenolic compounds and 3.220 mg QE/g of flavonoid. Moreover, extract showed higher antioxidant ability similar to butylated hydroxytoluene a standard molecule [(3.042±0.019) mg AAE/g and (4.12±0.10) mg AAE/g to saline extract and butylated hydroxytoluene, respectively]. The antimicrobial assays demonstrated that the extract had a significant antifungal potential against Candida species and could be used with safety against mice splenocytes, in concentrations lower than 50 μg/mL, promoting higher proliferation in these cells. Conclusions: Saline extract from Tithonia diversifolia leaves presents potential antioxidant, antifungal properties and induces immunostimulation in mice splenocytes.