Objective To evaluate the value of small intestinal double contrast radiography ( SIDCR) in the diagnosis of small intestinal diseases. Methods The data of 1182 SIDCR cases have been reviewed, including patients' sex, age, clinical symptoms and final diagnosis. The positive rate of diagnosis and types of disease in SIDCR cases were analyzed. Results The positive rate of diagnosis by SIDCR was 41.46%. In all positive cases, diverticulum accounts for 27.14%, inflammatory diseases 19.80%, and tumor 12.45%. The positive rate in in-patients was 54.00%, and in out-patients was 31.84%. The positive rate of male was 42.22%, and that of female 40.43%. The highest positive rate was 48.21% in 51-60 years old age group. In terms of clinical manifestations, the positive rate was highest in patients with abdominal mass (63.15%), and followed by gastrointestinal hemorrhage (51.58%) and abdominal pain (48.61%) respectively. Conclusions At present, the SIDCR is still a reliable and useful tech- nology, and is one of main methods for the diagnosis of small intestinal diseases.

Objective To observe the effectiveness of electrolyzed oxidizing water(EOW) in disinfections of endoscopes and compared with that of 2% glutaraldehyde. Methods (1) Disinfections of bacteri-a:Specimens were obtained immediately from endoscopes after being used, disinfected and washed out for bacterial culture. Endoscopes were immersed in either EOW or 2% glutaraldehyde for 1 , 3 and 5 minutes. Each time group consisted of 30 cases. (2 ) Effectiveness on disinfections of H. Pylori: endoscopes either from patients with H. Pylori infection or artificially contaminated with H. Pylori were washed out and exposed for 1 ,3 and 5 minutes in each of the disinfectants. PCR was used to detect DNA of H. Pylor. (3) Effectiveness of disinfections of HBV : Endoscopes were contaminated with HBV-positive serum, and the exposure time to both disinfectants were 1,3,5 and 10 minutes respectively. Thirty samples were studied in each time groups. Results ( 1 ) After manual cleaning and disinfections for 1, 3 and 5min in EOW, no vegetative bacteria were found. In 2% glutaraldehyde processing group, 5/30, 4/30 and 1/30 specimens remained bacteria positive, respectively. (2)There was no positive specimens found after 3 and 5 minutes' exposure in EOW in the group of H. Pylori, but 2/30 positive specimens were found in the 1 minute group. While 3/30, 4/30 and 4/30 positive specimens were found after exposure in 2% glutaraldehyde. (3) In the group of HBV, there is no positive case found after exposure in EOW, while in 2% glutaraldehyde groups, there were 2,5 ,4 and 5 cases remained positive in 1 ,3,5 and 10 minutes group respectively. Conclusion EOW is superior to 2% glutaraldehyde in disinfections of Hp or HVB with high-speed and thoroughness, and worthy to be recommended in disinfections of endoscopes.

Objective To investigate the prevalence and characteristic alterations of the peptic ulcer in Beijing area. Methods Retrospective analysis of clinical data of peptic ulcer collected from 29 hospitals of Beijing area in 1999. Results 8 832 cases of peptic ulcer were found from 64 874 cases of gastroscopy with detecting rate of 13.61%. Among these peptic ulcer patients,6 179 cases were found to have duodenal ulcer,accounting for 69.96% of the total cases of peptic ulcer;2 058 cases gastric ulcer,23.30%of the total cases with the ratio of 3:1 between these two subgroups.The rest 595 cases of other ulcer types , including complex ulcer and marginal ulcer accounted for 6.74% of the total cases.The detecting rates of peptic ulcer were 13.16%,19.11%, 13.23%,13.55%,20.59%in the tertiary hospitals, secondary hospitals (P

Objective To investigate the value of the high performance liquid chromatography (HPLC) with fluorescence detection of gastric juice in the diagnosis of gastric carcinoma. Methods After being super centrifuged, the supernatant of the gastric juice was chromatographed by reversed phase HPLC using C 18 column as the stationary phase and 20% methanol/H 2O as the mobile phase. The flow rate was set to 1 ml/min. The eluate was then detected with a fluorometer. Results 251 patients with different gastric diseases including 40 cases with gastric carcinoma were enrolled in our study. There were two characteristic peaks in the chromatograms, which appeared in both the benign and malignant lesions. The difference between benignancy and malignancy was the areas of the two peaks. The areas in the malignancy were much larger than those of the benignancy ( P

Objective To investigate the morbidity and Helicobacter pylori (H.pylori) reinfection status within 5 years after eradication. Methods One thousand and six adults aged between 35 to 37 were selected from general population in the high incidence region of gastric cancer. Among them, 552 individuals were confirmed to be H.pylori positive by both CLO test and Warthin Starry Stain histology. All the H.pylori positive individuals were randomly divided into two groups: treatment group receiving OAC triple therapy for one week and placebo group as controls. One month after cessation of treatment, the H.pylori eradication rate was 88.9%, while in the control group the H.pylori infection rate still remained 96.4% by 13 C UBT. These subjects were endoscopically followed up at the end of first and fifth years, respectively. H.pylori infected status was studied by histology and CLO test. Results 1. At 1 and 5 years after eradication, the response rates of subjects were 89.13% and 83.11%, respectively. 2. The morbidity rates of peptic ulcer, before treatment, were 9.87% and 7.61% in the treatment group and control group, respectively. At the 1st year, the morbidity rates were 3.70% and 12.85% in the two groups ( P

Objective To investigate the effects of antibody against heat shock protein (HSP) B of Helicobactor pylori (H. pylori) on H.pylori associated gastric disease for the study of the relationship between serum antibody to HSP B of H.pylori and the pathologic changes of the gastric mucosa. Methods 1 036 patients from Shandong province were collected. Six hundred patients were infected with H.pylori. Of those, three hundred received triple therapy for eradication of H.pylori and the other received placebo. Five years later, patients with H.pylori positive chronic superficial gastritis, chronic atrophic gastritis, peptic ulcer, and H.pylori negative chronic superficial gastritis underwent gastroscopy and collected serum specimens again, each group had 20 patients. The Western blot techniques were used to assay antibody to HSP B of H.pylori in sera of these patients. Results The H. pylori positive patients with chronic superficial gastritis ( 0.505 ? 0.061 ) had higher titers of anti H.pylori HSP B IgG antibody compared with those of gastric atrophy ( 0.448 ? 0.105 , P

Objective To investigate some specific fluorescent substances in gastric juice from patients with gastric carcinoma and to establish biomarkers for the detection of gastric cancer. Methods We collected gastric juice from 10 patients with gastric carcinoma and 10 patients without gastric carcinoma respectively. After being condensed, the specimens of gastric juice were analyzed by means of high performance liquid chromatography (HPLC). We collected the specific fluorescent substance in the retention time of 11.5-12.5 min in fluorescence spectra of HPLC and then detected them through mass spectrometry. Comparing such spectra with those of L-tryptophan, we attempted to find the principle component of the fluorescent substances. Results There was a specific substance in gastric juice in the retention time of 11.5-12.5 min in fluorescent spectra of HPLC, which was more abundant in gastric malignancy than in gastric benigh diseases. With the mass charge ratio(m/z)of 205, the substance had the same mass spectrum as shown in the third level of L-tryptophan, which was confirmed by mass spectrometry. This substance was identified to be L-tryptophan. Conclusions L-tryptophan is one of the specific fluorescent substances in gastric juice from patients with gastric carcinoma.

Objective To investigate the prevalence of gastric cancer and the relationship between H.pylori infection and the histopathologic changes of gastric mucosa in patients who had their H.pylori eradicated for 8 years in the high incidence region of gastric cancer. Methods One thousand and six adults(age from 35 to 75 years old)were randomly selected in Yantai area, Shandong province in 1996. Five hundreds and fifty two individuals with H.pylori positive were randomly divided into treatment group (n=276) and placebo group(n=276). Treatment group received triple therapy (omeprazol, amoxycillin and clarithromycin) for one week. The placebo group was served as control. One month after completion of treatment, H.pylori status was reassessed by 13C-UBT. Eradication rate of H.pylori in the treatment group was 89%.Follow-up study was carried out with endoscopy in the 2nd, 5th and 8th year, respectively. The endoscopic appearance and histology of the biopsies from the same site obtained at the first and final endoscopy were compared. Results ①At the 8th year′s follow up, 7 patients deve-loped gastric cancer, 1 in the H.pylori negative group(n=246)and 6 in the H.pylori positive group(n=306). The prevalence was 1/1968 person years in the treatment group and 6/2448 person years in placebo group(P0.05). ③ Eight years after eradication of H.pylori , the proportion of gastric atrophy in the body in the H.pylori eradication group was much lower than that in the non-eradication group(P0.05). Conclusions H.pylori infection increased the risk of gastric cancer occurrence, H.pylori eradication is able to reduce such risk. H.pylori eradication results in reduction in the pre-valence of gastric atrophy in the body, while continued H.pylori infection leads to progressive aggrava-tion of gastric atrophy and intestinal metaplasia.

Objective To evaluate the role of diammonium glycyrrhizinate(DG) in experimental colitis and to probe into its underlying mechanisms.Methods Tirty female Spragur-Dawley rats were randomized into treatment group,acetic acid control and normal control groups(10 rats for each group).Colitis in treatment group and acetic acid control was induced by administrating acetic acid rectally.The rats in treatment group were treated with DG.Disease activity index(DAI) and histological damage as well as myeloperoxidase(MPO) activation were observed.The nuclear factor-?B(NF-?B) activity,the(expressi)on of tumor necrosis factor-?(TNF-?) and intercellular adhesion molecule-1(ICAM-1) in colon tissues were detected by immunohistochemistry method.Results In treatment group,acetic acid control(and normal) control,the DAI were 3.5?0.6,7.1?0.8 and 0.5?0.4 respectively;the histological(damage) scores were 3.5?0.9,6.1?1.0 and 1.0?0.5 respectively;MPO activation were 0.72?0.08,2.02?0.10 and 0.22?0.04 respectively;the positive percentages of TNF-? were 35.2?8.2,62.5(?10.1) and 7.9?5.7 respectively,the positive percentages of ICAM-1 were 34.3?8.2,60.2?8.3 and 9.1?3.4 respectively;the positive percentages of NF-?B were 23.3?9.2,44.5?8.9 and 9.6?4.4(respectively).(Compared) with the acetic acid control group,the DAI and histological damage scores as well as MPO(activation) of colon tissues in treatment group were significantly improved(P

Objective The aim of this study was to assess the reliability of a newly developed enzymeimmumoassay, the Helicobacter pylori (H. pylori) stool antigen (HpSA) test, for the detection of H.pylori infection before and after eradication. Methods The H.pylori infected patients referred to our department were included. They were divided into two groups. The 331 patients in group A had intact stomach, and 65 patients in group B had history of subtotal gastrectomy. All patients underwent gastroscopy with biopsies for rapid urease test (RUT) and histology, which was viewed as “gold standard”. H.pylori status was defined as positive or negative with both RUT and histology presenting concordant positive or negative results. The results of these reference tests were compared with those obtained by HpSA test and ()~(13)C-urea breath test (()~(13)C-UBT). In addition, Fifty-six-positive patients in group A, constituting group C, were treated with 1-week triple therapy. At the 28 th day after the end of therapy, the patients underwent another ()~(13)C-UBT which was also defined as “gold standard”. The stool specimens were collected on days 1, 7, 14, 21, and 28 after completion of therapy and were used to detect the antigen of H.pylori by HpSA. Results In group A, 175 patients were defined as H.pylori-positive and 156 as negative by the “gold standard”. The sensitivity and specificity of the HpSA test was (95.4%) and 91.0%, respectively. There was no significant difference between HpSA test and ()~(13)C-UBT. In group B, 30 patients were defined as H.pylori-positive and 35 as negative by the “gold standard”. The sensitivity of the HpSA test and ()~(13)C-UBT was 90.0% and 66.7%, respectively (P