Based on the properties of the zirconia all-ceramic material,this report presented a clinical application with the zirconia all-ceramic crown/bridge for restoration of multiple residual roots and crowns.

Objective: To observe the effects of acid etching times on nano-leakage and microtensile bond strength(?TBS) of the 4 total-etching adhesives-dentin bond.Methods: Superficial occlusal dentin surfaces of 80 molars were prepared with 600-grit SiC paper under running water.4 adhesives were applied to dentin surfaces under different etching time.The bonded teeth were sectioned occluso-gingivally into 1.0 mm?1.0 mm?4.0 mm serial slabs which were prepared for TEM and ?TBS tests.Results: Under 15 s etching time for 4 adhesives,the highest ?TBS(OB(25.36?4.18) MPa,SB(24.25?3.97) MPa,OS(28.65?4.93) MPa,PB(27.12?4.13) MPa) were achieved,and the amount of nanoleakage was positively correlated with the length of etching time.Conclusion: Etching for a reasonable time demineralizes the dentin surface to a moderate depth,resulting in higher ?TBS and lower nanoleakage.

Objetives: To evaluate the microtensile bond strengths o f four dentin adhesives (Single Bond, Bond-1, Prime&Bond NT and One-Step) i n vivo.Methods: Before extraction the superficial occlu sal dentin of 19 molars (because of severe periodontitis) in 17 volunteers was exposed by diamond bur of a high-speed hand-piece copious air-water spray under local anesthesia, the crown was bonded with one of the above adhesives a ccording to the manufacturers' instructions. The teeth were then extracted and kept in normal saline for 24 h under 37 ℃,sectioned to obtain two or three bar -shaped specimens, which were then shaped to an hour-glass form. The specimens were stressed until rupture of the bond. The mean bond strength of the adhesive s was measured and compared using student test. Rusults: M ean microtensile bond strength (MPa) of Single Bond,Bond-1,One-Step and Prime & Bond NT to the tooth surface was 26.18?3.67,30.08?3.15,29.91?3.51,29.70?3 .03 respectively (P

Objective:To introduce a new fixation set-up for micro-tensile test.Methods:Dentin-composite were bonded with AdperTM Single Bond 2 (SB2)and sectioned into stick-shaped specimens.Specimens from each tooth(n =6)were equally divided into Ciucchi's jig and the designed set-up(Control and experimental)groups for micro-tensile bond test according to the utilized fixa-tion set-up.The bonding interface failure mode was examined with field-emission scanning electron microscope (FESEM).Three-dimensional models of the two set-ups and the specimen were developed,stress distribution was analyzed by finite element analysis (FEA).Results:The bond strength(MPa)of experimental and control group was 32.76 ±7.43 and 43.58 ±4.72(P <0.05),the ratio of mixed failure was 28 /36 and 20 /36(P <0.05)respectively.FEA showed that the designed set-up for fixing the sticks pro-vided a uniform stress distribution along the long axis of the specimen.FEA and failure mode analysis confirmed such uniform distri-bution was also concentrated at the bonding interface.Conclusion:The new set-up is feasible for micro-tensile test.

Objective To evaluate the colonoscopy surveillance in colorectal cancer patients after surgical removal of the tumor.Methods From June 1986 to June 2007,2762 asymptomatic patients who had underwent operation for colorectal cancer were put into colonoscopy surveillance.They had the first examination 3-6 months after the operation,and were re-examined once a year thereafter for 3 years.The follow-up findings were compared with those from the 218 symptomatic patients who had colorectal cancer surgery from September 1981 to May 1986.Results In 2762 asymptomatic patients,48 cases of multiple primary cancer were detected,in which 39 cases(1.4%) were found at one examination and 9 cases(0.3%)at different examination.The TNM staging of these lesions included stage Ⅰ in 6,stage Ⅱ in 31 and stage Ⅲ in 11.During the surveillance,583 cases(21.1%) of adenoma were detected and endoscopically resected,in which 17(3.2%) were invasive early cancer and 58(9.9%) were high grade dysplasia.In 218 patients with symptoms,29 cases(13.3%) of adenoma and 27 cases( 12.4%) of cancer were detected,including 4 cases of stage Ⅰ cancer,6 of stage Ⅱ and 16 of stage Ⅲ.Conclusion Colonoscopy surveillance in colorectal cancer patients after surgery is important in finding precancerous lesion and early stage cancer,and is recommended in all patients.

Objective To investigate the effect of four different cements on the bonding effectiveness of root canal dentine and fiber post before and after different treatments.Methods A total of 216 freshly extracted sound single-root-canal mandibular premolars were randomly divided into four groups.After root canal treatment and post space preparation being conducted on the premolars,Fuji Ⅰ,Fuji Cem,RelyX Unicem,RelyX ARC were used respectively to bond fiber posts and were marked with group A,B,C,and D.Microleakage,micromorphology of the bonded interfaces,and pull-out bond strength were evaluated in the immediate group,thermocycling group and thermomechanical loading group.Results In the immediate group,samples in group D showed the highest bond strength [(278 ± 26) N],followed by group C[(219 ±12) N],B[(104 ±23) N] and A[(73 ±8) N].Significant differences were found among all groups (P ＜ 0.05).A significant increase in bond strength was found in group A and B,whereas a decrease tendency was detected in group C and D after different treatments.Scanning electron microscope indicated that some little gaps were observed in group D after treatment,while a more intense bonding interface was found in group A and B.Microleakage scores in group A and B were lower than those in group C and D after aging treatments.Conclusions Resin cement can achieve a better immediate bond strength,while resinmodified resin cement may acquire a better long-term retention.

Objective To introduce the method of dual immunofluorescence labeling of human dentin matrix without demineralization of the whole dentin fragments, and to analyze the distribution of type-Ⅰ collagen fibrils and chondroitin sulfate in human dentin.Methods Forty 30 μm-thick middle coronal dentin sections were obtained from 8 freshly extracted human third molars and etched with 37％ phosphoric acid(PA) gel for 15 s.After preconditioning with or without tosyl-phenylalanyl chloromethyl ketone(TPCK) treated trypsin digestion, sections were subjected to dual immunofluorescent labeling and scanned by confocal laser scanning microscopy to identify the type-Ⅰ collagen fibrils and chondroitin sulfate.Results Chondroitin sulfate was localized in the lumens of the dentin tubules and peritubular dentin, while the type-Ⅰ collagen fibrils were localized in intertubular dentin and peritubular dentin.After preconditioning with TPCK treated trypsin digestion, the red fluorescence was decreased or disappeared.Conclusions The dual immunofluorescence labeling methodology can be used to study the human dentin matrix without demineralization of the whole dentin fragments.Chondroitin sulfate was localized in the lumens of the dentin tubules and peritubular dentin, while the type-Ⅰ collagen fibrils were localized in intertubular dentin and peritubular dentin.

OBJECTIVETo investigate the differences of clinical characteristics and molecular features among colorectal cancer subsides and provide evidence for colorectal cancer protection, diagnosis and treatment.

METHODSAll of 4 316 colorectal patients from Shanghai cancer center were selected for clinical character analysis, among which, 2 224 subjects for molecular feature analysis. Clinic pathological characteristics like age, gender, tumor types, histological types, differentiation and T-stage, as well as molecular features like hMLH1, hMSH2, CD44, p21, p53, COX2,E-cadherin, Her2 and Ki-67, were involved into this research.

RESULTSIt showed that compared with left-sided colon and rectal cancers, right-sided cancers occurred more in women (46.0% (541/1 176); 39.2% (424/1 083); 41.2% (848/2 057), respectively, χ² = 11.85, P < 0.01), had more mucinous or signet-ring carcinoma (12.0% (128/1 064), 5.8% (56/960), 4.0% (75/1 859), respectively, χ² = 31.27, P < 0.01), poor differentiated carcinoma (32.1% (343/1 069), 19.5% (201/1 033), 19.3% (380/1 967), respectively, χ² = 72.66, P < 0.01) , and advanced T stage (87.9% (992/1 129), 83.2% (869/1 045), 72.2% (1 486/2 057), respectively, χ² = 121.44, P < 0.01). Meanwhile, the rates of hMLH1 were higher in right-sided colon cancers when compared with rectal cancers (13.4% (59/439) vs 8.5% (88/1 035), OR (95%CI): 1.67 (1.18-2.37)), as well as the rates of hMSH2 negative expression (4.9% (22/452) vs 2.4% (26/1 083), OR (95% CI): 2.08(1.17-3.71)). The rates of p53 positive expression were higher in right-sided colon cancers when compared with rectal cancers (76.2% (321/421) vs 68.4%, (776/1 134), OR (95% CI): 0.68 (0.52-0.87)). Compared with right-sided colon cancers, the rates of Her2 positive expression were higher in rectal cancers (19.3% (176/913) vs 13.2% (45/340), OR (95% CI): 1.57 (1.10-2.23)) , as well as the rates of Ki-67 expression which was positive in more than 50% cells (73.6% (840/1 141) vs 65.6% (299/456), OR (95% CI): 0.68 (0.54-0.86)).

CONCLUSIONThere are specific characteristics in right-sided colon cancers. The difference of molecular features between right-sided colon and rectal cancers are more significant.

Adenocarcinoma, Mucinous ; Cadherins ; Carcinoma ; Carcinoma, Signet Ring Cell ; China ; Colonic Neoplasms ; Colorectal Neoplasms ; Female ; Humans ; Neoplasm Grading ; Neoplasm Staging ; Rectal Neoplasms ; Sex Factors

Objective To compare the effects of bone transport versus induced membrane technique for large segmental tibial defects.Methods The clinical data were analyzed retrospectively of 89 patients with large segmental tibial defect who had been treated at Department of Orthopaedics,Wuxi No.9 People's Hospital from June 2005 to February 2017 using bone transport or induced membrane technique.They were 58males and 31 females,aged from 13 to 74 years (average,38.0 years).The bone transport group had 59cases and the induced membrane technique group 30 cases.The 2 groups were compared in terms of preoperative general data and postoperative bone nonunion,bone healing time,complications and functional recovery of the adjacent joint.Results There were no statistically significant differences between the 2groups in terms of age,gender,cause or type of defects,associated injury,course of disease,functionary scores of the adjacent joint or number of operations,showing compatibility between the 2 groups (P ＞ 0.05).All the patients were followed up for 12 to 48 months (average,20 months).The bone transport group had significandy longer clinical healing time (14.7 ± 5.4 months) and significantly higher incidences of major complications (50.8％),minor complications (57.6％) and overall complications (83.1％) than the induced membrane technique group (11.2 ± 2.8 months,16.7％,26.7％ and 30.0％,respectively) (P ＜ O.05),but significantly lower functionary scores of the adjacent joint (86.4 ± 5.0 points) than the induced membrane technique group (88.8 ± 4.9 points) (P ＜ 0.05).Conclusions Both bone transport and induced membrane technique are effective repairs for large segmental tibial defects.However,induced membrane technique may be superior to bone transport in terms of bone healing,complications and functional recovery.

Objective To investigate the differences of clinical characteristics and molecular features among colorectal cancer subsides and provide evidence for colorectal cancer protection,diagnosis and treatment.Methods All of 4 316 colorectal patients from Shanghai cancer center were selected for clinical character analysis, among which, 2 224 subjects for molecular feature analysis.Clinic pathological characteristics like age, gender, tumor types, histological types, differentiation and T-stage, as well as molecular features like hMLH1,hMSH2,CD44,p21,p53,COX2,E-cadherin,Her2 and Ki-67,were involved into this research.Results It showed that compared with left-sided colon and rectal cancers, right-sided cancers occurred more in women ( 46.0% ( 541/1 176 ); 39.2% ( 424/1 083 ); 41.2% ( 848/2 057 ) , respectively,χ2 =11.85,P<0.01), had more mucinous or signet-ring carcinoma(12.0%(128/1 064), 5.8%(56/960),4.0%(75/1 859),respectively,χ2 =31.27,P <0.01), poor differentiated carcinoma (32.1%(343/1 069),19.5%(201/1 033), 19.3%(380/1 967),respectively,χ2 =72.66,P<0.01), and advanced T stage(87.9%(992/1 129), 83.2%(869/1 045), 72.2%(1 486/2 057),respectively,χ2 =121.44,P <0.01).Meanwhile,the rates of hMLH1 were higher in right-sided colon cancers when compared with rectal cancers ( 13.4% ( 59/439 ) vs 8.5% ( 88/1 035 ) , OR ( 95%CI ): 1.67 ( 1.18-2.37)),as well as the rates of hMSH2 negative expression(4.9% (22/452) vs 2.4% (26/1 083),OR (95%CI):2.08(1.17-3.71)).The rates of p53 positive expression were higher in right-sided colon cancers when compared with rectal cancers ( 76.2% ( 321/421 ) vs 68.4%, ( 776/1 134 ) , OR ( 95%CI ): 0.68 (0.52-0.87)).Compared with right-sided colon cancers,the rates of Her2 positive expression were higher in rectal cancers(19.3%(176/913) vs 13.2% (45/340),OR(95%CI):1.57 (1.10-2.23)),as well as the rates of Ki-67 expression which was positive in more than 50%cells (73.6%(840/1 141) vs 65.6%(299/456),OR(95%CI):0.68 (0.54-0.86)).Conclusion There are specific characteristics in right-sided colon cancers.The difference of molecular features between right-sided colon and rectal cancers are more significant.