1.Investigation of antigen related to the in vitro invasiveness of salmonella typhimurium through the Madin-Darby canine kidney(MDCK) epithelial cell monolayer.
Chung Gyu PARK ; Eung Soo HWANG ; Ju Young SEOH ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1991;26(6):553-562
No abstract available.
Epithelial Cells*
;
Salmonella typhimurium*
;
Salmonella*
2.A Comparison of Inhibitory Effects in Brown and White Rice (Oryza sativa L.) against Mutagenicity Induced by Tryptophan Pyrolysates.
Jung Eun YOU ; Hyang Sook CHUN ; Jung Soon CHO
Journal of the Korean Dietetic Association 1997;3(2):105-111
The inhibitory effect of rice(Oryza sartiva L., illpumbyeo) against mutagenicity induced by tryptophan pyrolysates were investigated using Salmonella typhimurium reversion assay. Both methanol extracts of obtained from brown and white rice were found to possess strong activites of inhibiting the mutagenicities of 3-amino-1,4-dimethyl-5H-pyriod[4,3-b]indol(Trp-P-1) and 3-amino-1-methyl-5H-pyrido[4,3-b]indol(Trp-P-2) on Salmonella typhimurium reversion assay. As the concentration of methanol extract increased, inhibitory effect on mutagenicity increased but reached at steady state as inhibition rate of 90% when the concentration was above 10mg/plate. There was no significant difference(p>0.05) in inhibitory effect of methanol extracts between brown and white rice against tryptophan pyrolysates.
Methanol
;
Salmonella typhimurium
;
Tryptophan*
3.Study on survival time of microorganisms in fecal material in urine-diverting eco-san toilets
Journal of Preventive Medicine 2002;12(3):44-49
Salmonella typhimurium phage 28B and Ascaris suum ova were used as indicators to evaluate the ability of pathogen destruction of 6 types of dry toilets and the retention time of fecal material processing vault needing for health safety. The results showed that: among three factors: pH, temperature, moisture, pH was the most significantly influenced on the survival time of indicators. When pH>10, Salmonella phages and Ascaris ova were destroyed very fast. These pH values could be reached if after defecation 300mL of wood-ash was added. At least 6 months of retention for fecal materials inside dry toilets are needed
Hygiene
;
Salmonella typhimurium
4.In vitro infection of murine macrophages with salmonella typhimurium and listeria monocytogenes.
Hyun Chul LEE ; Jung Sook PARK ; In Chol KANG ; Sung Sik PARK
Journal of the Korean Society for Microbiology 1991;26(6):499-505
No abstract available.
Listeria monocytogenes*
;
Listeria*
;
Macrophages*
;
Salmonella typhimurium*
;
Salmonella*
5.Electron microscopic study on the response of the intestinal mucosa and macrophage to invasion of salmonella typhimurium.
Sung Sik PARK ; Sang Sook LEE ; Hyun Chul LEE
Korean Journal of Anatomy 1991;24(4):489-502
No abstract available.
Intestinal Mucosa*
;
Macrophages*
;
Salmonella typhimurium*
;
Salmonella*
6.Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis Identified by Multiplex PCR in Korea.
Soo Jin YANG ; Kyoung Yoon PARK ; Keun Seok SEO ; Thomas E BESSER ; Yoon Ho KOOK ; Han Sang YOO ; Yong Ho PARK
Journal of the Korean Society for Microbiology 2000;35(5):366-366
No Abstract Available.
Korea*
;
Multiplex Polymerase Chain Reaction*
;
Salmonella enteritidis*
;
Salmonella typhimurium*
;
Salmonella*
7.Mutagenicity of Human Urine Excreted after Ingestion of Roast Beef.
Dong Gu SHIN ; Jung Hee KIM ; Jae Ryong KIM
Yeungnam University Journal of Medicine 1987;4(2):105-111
This study was undertaken to observe the mutagenic occurrence in urine excreted after the ingestion of roast beef. Two healthy nonsmoker persons of both sex were selected for this test, employing two strains (TA98, TA100) of Salmonella typhimurium according to Ames' method. The mutagenic activity began to appear in urine of both sex three hours after ingestion of 300 g of roast beef, gradually increasing until 6 hours and declining thereafter.
Eating*
;
Humans*
;
Methods
;
Red Meat*
;
Salmonella typhimurium
8.Capability of acidic electrolyzed water in the elimination of Salmonella Typhimurium and Escherichia coli in the chicken breast
Mahmoud Ammar Mohamed Ammar ; Mohamed Hamdy Mohamed
Malaysian Journal of Microbiology 2022;18(3):282-290
Aims:
This study aimed to investigate the effect of acidic electrolyzed water (AEW) as pre-refrigeration and pre-freezing processing steps for chicken meat in regard to the behavior of S. Typhimurium and E. coli during storage.
Methodology and results:
AEW (free available chlorine 30 ppm and pH 2.7) was tested against S. Typhimurium and E. coli in growth media (brain heart infusion broth) and by exposing inoculated chicken fillets. The in vitro study appointed 10 minutes as the straightening exposure time of fresh prepared AEW for S. Typhimurium and E. coli. The reduction effect of AEW was significant (p<0.05) for both S. Typhimurium and E. coli along the 8 days of refrigerated storage with a maximum reduction after 24 h of post-treatment reaching 23.3% (1.4 log CFU/g) and 32.43% (2.15 log CFU/g) for S. Typhimurium and E. coli, respectively. AEW resulted in a significant reduction (p<0.05) as a pre-freezing application for both microorganisms, where the maximum reductions of 20% (1.2 log CFU/g) and 31.84% (2.14 log CFU/g) for S. Typhimurium and E. coli, respectively, were reported at zero time (just after dipping). In exposed samples to AEW, S. Typhimurium could not be detected by the 6th week of frozen storage while E. coli continued detectable until till 10th week but with a reduced population of 30% compared to control.
Conclusion, significance and impact of study
The findings of the present study suggest the application of AEW as a pre-refrigeration and pre-freezing treatment for chicken products. AEW application significantly improved the safety of chicken products.
Electrolytes
;
Chickens--microbiology
;
Salmonella typhimurium
;
Escherichia coli
9.Evaluation of the efficiency of ciprofloxacin against S. Typhi by altering the production of cytokines in acute typhoid fever in patients at Al-Diwaniyah Hospitals, Iraq
Baleegh A Kadhim ; Zeayd Fadhil Saeed ; Sura A Al-Ganahi
Malaysian Journal of Microbiology 2022;18(5):562-570
Aims:
Typhoid fever is a life-threatening disease in the developing world that claims >600,000 deaths per year. Its causative agent Salmonella Typhimurium (S. Typhi) can be treated with ciprofloxacin, an effective broad-spectrum antibiotic that enhances the natural host defenses. However, the emergence of resistant bacterial strains may be a warning alarm against the clinical use of this antibiotic. This study was aimed to investigate the efficiency of ciprofloxacin treatment (250 mg/mL) against S. Typhi by altering the production of serum cytokines IL-10, 1L-6 and TNF-α in acute typhoid fever patients in Diwanyah Hospitals.
Methodology and results:
ELISA and Western Blot methods were used to investigate cytokine levels in patients and healthy controls sera. Our results showed that all cytokines’ levels before treatment with ciprofloxacin were significantly higher than the control (healthy group). However, treated patients with ciprofloxacin revealed a significantly reduced concentration of IL-10 and TNF-α compared to untreated control samples. However, the level of IL-6 was higher even with ciprofloxacin treatment.
Conclusion, significance and impact of study
The study concluded that ciprofloxacin (250 mg/mL) might significantly alter serum cytokines IL-6, IL-10 and TNF-α levels in acute typhoid fever patients. Therefore, further molecular studies are essential to understand the effect of ciprofloxacin on the production of cytokines.
Typhoid Fever
;
Ciprofloxacin
;
Salmonella typhimurium
;
Cytokines
10.Transcriptional Analysis of the iagB within Salmonella Pathogenicity Island 1 (SPI1).
Sangyong LIM ; Jihae CHOI ; Dongho KIM ; Ho Seong SEO
Journal of Bacteriology and Virology 2016;46(3):128-134
HilA is a central regulator of Salmonella pathogenicity island 1 (SPI1), which is necessary for host invasion by Salmonella and induction of gastroenteritis. The iagB lies downstream of hilA and is thought to be co-transcribed with hilA, but iagB expression has not yet been analyzed directly. In this study, iagB expression in various mutant strains was measured to determine whether the expression pattern was similar to that of hilA. A β-galactosidase assay revealed that iagB expression was greater under shaking than standing culture condition. iagB expression was decreased in relA/spoT and ihfB mutants but not in luxS mutant, in line with previous reports on hilA expression. The hilA and iagB mRNA levels decreased by approximately 2-fold in arcA mutant grown aerobically and increased by approximately 10-fold in fnr mutant grown anaerobically. Although the fold changes in hilA and iagB mRNA level differed in hfq mutant strain, the patterns of time- and Hfq-dependent regulation were similar for both genes. Thus, iagB and hilA exhibited similar expression patterns in various mutational backgrounds and under different growth condition.
Gastroenteritis
;
Genomic Islands*
;
RNA, Messenger
;
Salmonella typhimurium
;
Salmonella*
;
Virulence*