1.The Experiences of How to Improve the Teaching Quality of Neurology
Chinese Journal of Medical Education Research 2006;0(11):-
The teaching of Neurology is a emphasis and difficulty in clinical teaching.In the present article,we summarized some experiences of how to improve the teaching quality of Neurology.
2.Effect of Ginkgo biloba extract on serum DMT1, grp75 and neurological function in patients with Parkinson disease
Fei WEI ; Jie SHUAI ; Lin SHEN ; Zili GONG ; Saiyu CHENG
Chinese Journal of Biochemical Pharmaceutics 2015;(8):72-74
Objective To investigate the effect of Ginkgo biloba extract on serum divalent metal transporter1 ( DMT1 ) , glucose regulated protein 75(grp75) and nerve function in patients with Parkinson disease.Methods 38 cases of patients loith parkinson disease according to different drugs were divided into experimental group and control group, 19 cases in each group.Control group was treated with levodopa and Benserazide tablet, experimental group on the basis of control group, was given Ginkgo biloba extract tablets, treatment for 4 weeks.After treatment, DMT1, grp75 and cognitive function of all patients in substantia nigra were detected.ResuIts Compared with before treatment, two groups of patients with lower DMT1 level (P<0.05), compared with control group, experimental group of patients with lower DMT1 levels (P<0.05).Compared with pre-treatment, two groups of patients grp75 level was higher (P<0.05), compared with control group, experimental group after treatment grp75 level was higher (P<0.05).Compared with before treatment, the two groups of patients with MoCA scores were higher (P<0.05), HAMD scores were lower (P<0.05).Compared with control group, experimental group after treatment MoCA scores were higher (P<0.05), HAMD scores were lower (P<0.05).ConcIusion Ginkgo biloba extract can significantly reduce the level of DMT1 in the substantia nigra of Parkinson patients, increase the level of grp75, and improve the cognitive function.
3.Effect of hypoxia on the neurons expression of P2X receptors in CA1 subfield of rat hippocampus
Yandong ZHAO ; Saiyu CHENG ; Jinhai ZHANG ; Huaizhen RUAN
Journal of Third Military Medical University 2003;0(23):-
Objective To investigate the expression of P2X receptors in CA1 subfield of rat hippocampus and the effect of hypoxia on the expression. Methods We set up the hypoxia animal model of high altitude and observed the expression of P2X receptors by using immunohistochemical staining before and after hypoxia. Results Immunohistochemistry showed that there were several sub-type of P2X receptors expressed in hippocampus CA1 neurons. After long term hypoxia, the expression of these receptors was increased. Conclusion there were abundant of P2X receptors expressed on the hippocampus CA1 neurons. Hypoxia affects the expression P2X receptors.
4.Chitin enhances neural reconstruction after sciatic nerve injury in rats
Saiyu CHENG ; Huaizhen RUAN ; Xigui WU ; Jinhai ZHANG
Journal of Third Military Medical University 2003;0(20):-
Objective To investigate the effects of chitin on the functional recovery after sciatic nerve axotomy. Methods Upon silicone-tubulization of the transected sciatic nerve in the adult rats, either 0.9% saline or 1% chitin solution was injected into the silicone chamber. The status of functional recovery of the injured sciatic nerve was observed by electrophysiological analysis, hydrogen-peroxide oxidoreductase (HRP) retrograde trace method, and axon morphometric analysis at 30 and 90 d respectively after sciatic nerve transection. Results ① Chitin shortened the latent period of CMAP by 1.79 ms and 1.29 ms, promoted the nerve conduction velocity by 16.00 m/s and 22.00 m/s, enhanced the amplitude by 8.17 mv and 12.42 mv, respectively, at 30 and 90 d after sciatic nerve transection (P
5.Construction and identification of rat GDNF gene recombinant retroviral vector and gene transfection to NSC.
Saiyu CHENG ; Huaizhen RUAN ; Zhong YANG ; Xigui WU
Journal of Biomedical Engineering 2008;25(3):642-646
By genetic recombinant technique, the rat GDNF cDNA was recombinated to the retroviral vector pLXSN. The recombinant plasmid pLXSN-GDNF was verified by digestion with restriction endonucleases and PCR. Then neural stem cells (NSCs) were infected with pLXSN-GDNF. Immunocytochemistry, RT-PCR and western-blot were used to detect the transfection effect. Results showed that GDNF cDNA was cloned into retroviral vector pLXSN correctly, and the pLXSN-GDNF can infect NSCs efficiently. These results provide the possibility for transplantation and gene therapy with GDNF of nervous system diseases and injury.
Adenoviridae
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genetics
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Animals
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Cloning, Molecular
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DNA, Complementary
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genetics
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Gene Transfer Techniques
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Genetic Therapy
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Genetic Vectors
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genetics
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Glial Cell Line-Derived Neurotrophic Factor
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genetics
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Neurons
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cytology
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Rats
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Recombinant Proteins
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genetics
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Stem Cells
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cytology