Through the literature collection on Chinese marine materia medica,this study analyzed medication rules of Chinese marine materia medica prescription,understood general conditions of Chinese marine materia medica prescription,in order to conduct data mining on medication rules of Chinese marine materia medica prescription.The name of Chinese marine materia medica was used as the search term.Chinese marine materia medica prescriptions were searched in related literatures of Chinese Medicine Code,Chinese Marine Materia Medica,Chinese Materia Medica,Chinese Pharmacopoeia and Great Dictionary of Chinese Medicine.The information was extracted and standardized to construct database for the initial data mining of related information and medication rules of Chinese marine materia medica prescriptions.The results showed that 16715 Chinese marine materia medica prescriptions were screened,which contained 144014 items of data,involving 218 kinds of Chinese marine materia medica.Decoction was the most common dosage form.The amount of Chinese marine materia medica prescription in the Ming and Qing dynasties was the largest.The highest frequency of Chinese marine materia medica in one prescription was 1 to 3 types.The prescription composed all by Chinese marine materia medica occupied 8.065％.Other prescriptions contained the compatibility of Chinese terrestrial materia medica.The prescription containing materia medica half from the sea and half from the land,occupied 7.754％.The Chinese marine materia medica used with the highest frequency in all prescriptions was oyster.The frequently used Chinese terrestrial materia medica was licorice and angelica in Chinese marine materia medica prescriptions.It was concluded that the number of Chinese marine materia medica prescription was large.Its compatibility and clinical application had a certain characteristic,which provided data foundation for the further research and development of Chinese marine materia medica.

Thyroid B-ultrasound and urinary iodine content were examined in 4390 male workers aged 18-62 years-old,who participated annual physical checkups in 2009 and 2010.The results suggest that there is no obvious relationship between the urinary iodine which represents the iodine intake within 24 hours and prevalence of thyroidnodale.However,the prevalence of thyroid nodules shows an upward trend with the advance of age.

Objective: To prepare the monoclonal antibody against ankyrin repeat domain 22（ANKRD22）and to investigate its expression in colorectal cancer tissues. Methods : The recombinant human ANKRD22 was expressed through E. coli and pET-42a and then used to immunize Balb/c mice after purification. Anti-human ANKRD22 specific monoclonal antibodies were selected by Western blotting with 293T cell lysate highly expressing ANKRD22 as antigen. The expression of ANKRD22 in the tissue microarrays of 112 patients with colorectal cancer was detected by immunohistochemical staining. Results : Four specific monoclonal antibodies against human ANKRD22 were screened out of 93 hybridoma cells,which reacted well with natural human ANKRD22. ANKRD22 was mainly distributed in the cytoplasm of colorectal cancer cells. In 112 cases of colorectal cancer,94 cases were detected positive for ANKRD22 expression,with the positive rate of 83.93%. The expression of ANKRD22 was statistically correlated with the expression of p53 and β-catenin（P<0.05）,but not with age,sex,location of tumors,AJCC stage,Dukes stage,degree of differentiation,lymph node metastasis and mismatch repair gene expression（P>0.05）. Conclusion The expression level of ANKRD22 was high in colorectal cancer. ANKRD22 might be involved in the carcinogenesis of colorectal epithelium and be a potential diagnostic marker.

Objective:To explore the risk factors of diabetic nephropathy(DN).Methods:From January 2016 to September 2019, the clinical data of 403 patients with type 2 diabetes in the Integrated Traditional Chinese and Western Medicine Hospital of Wenzhou were analyzed retrospectively.Excluding other factors, they were divided into DM group (315 cases) and DN group(88 cases) according to whether or not they were complicated with DN.The age, course of disease, complications, monocyte to high-density lipoprotein cholesterol ratio(MHR) and other test data were included in the logistic regression model to analyze the independent risk factors of DN.Results:There were statistically significant differences in the course of ≥10 years[95 cases(30.16%) vs.37 cases(42.05%)], complicated with hypertension[141 cases(44.76%) vs.58 cases(65.91%)], insulin application[94 cases(29.84%) vs.39 cases(44.32%)], ACEI/ARB application[132 cases(41.90%) vs.62 cases(70.45%)], FBG[(7.38±2.45)mmol/L vs.(8.26±2.06)mmol/L], HbA1c≥7%[91 cases(28.89%) vs.36 cases(40.91%)], MHR[(10.30±4.38) vs.(17.10±7.18)] and HDL-C[(0.58±0.16)mmol/L vs.(0.52±0.17)mmol/L] between the two groups( t=4.41, 13.31, 6.52, 22.46, 3.08, 4.60, 11.02, 2.65, all P<0.05). Multivariate logistic regression analysis showed that hypertension( OR=2.06, 95% CI=1.36-2.76), HbA1c≥7%( OR=1.32, 95% CI=1.04-1.60), MHR≥11.78( OR=1.65, 95% CI=1.03-2.67) were independent risk factors for DN. Conclusion:Hypertension, HbA1c ≥7%, MHR ≥11.78 are independently correlated with DN, which provide a basis for clinical prognosis.

Objective:To explore the relationship between sex hormone binding globulin and glycolipid metabolism in patients with obese polycystic ovary syndrome.Methods:From April 2016 to April 2019, 114 patients with obese polycystic ovary syndrome in the Integrated Chinese and Western Medicine Hospital of Wenzhou were selected and divided into two groups according to the level of sex hormone binding globulin, including 66 cases in the low level group and 48 cases in the normal group.The blood glucose, blood lipid and sex hormone levels of the two groups were measured and the results were analyzed.Results:The levels of TC, TG, LDL-C and ApoB in the low level group[(2.32±0.97)mmol/L, (6.45±1.25)mmol/L, (3.57±1.01)mmol/L, (0.93±0.32)g/L] were significantly higher than those in the normal group[(1.87±0.89)mmol/L, (4.88±0.94)mmol/L, (3.12±0.88)mmol/L, (0.76±0.17)g/L]( t=2.477-7.322, all P<0.05). The level of ApoA in the low level group[(1.22±0.34)g/L] was significantly lower than that in the normal group[(1.58±0.47)g/L]( t=-4.748, P<0.05), while there was no statistically significant difference in HDL-C between the two groups[(1.45±0.31)mmol/L vs.(1.43±0.56)mmol/L, t=0.244, P>0.05]. The levels of FPG, HbA1C and FINS in the low level group[(5.53±1.45)mmol/L, (6.42±0.79)% and (18.71±5.46)mU/L] were significantly higher than those in the normal group[(5.09±0.76)mmol/L, (6.01±0.92)% and (15.49±4.98)mU/L]( t=2.136-3.225, all P<0.05), while there was no statistically significant difference in 2hPG between the two groups[(8.79±1.56)mmol/L vs.(8.32±1.84)mmol/L]( t=1.472, P>0.05). The incidence of insulin resistance in the low level group[54.55%(36/66)] was significantly higher than that in the normal group[35.42%(17/48)](χ 2=4.088, P<0.05). The level of T in the low level group[(61.45±21.44)ng/mL] was significantly higher than that in the normal group[(50.39±22.31)ng/mL]( t=2.673, P<0.05), while there were no statistically significant differences in FSH, LH, E 2 and PRL between the two groups( t=0.427-1.714, all P>0.05). Conclusion:The patients with low levels of sex hormone binding globulin are more likely to suffer from hyperglycemia and hyperlipidemia, and low levels of sex hormone binding globulin may be a risk factor for obese polycystic ovary syndrome.

Objective:To investigate the efficacy and safety of ixazomib-based therapy for multiple myeloma.Methods:The data of 32 patients with multiple myeloma treated with isazomib-based regimen in the Affiliated Hospital of Jining Medical University from December 2020 to December 2021 were retrospectively analyzed. Among 32 patients, 17 cases were relapsed/refractory, and the remaining 15 cases had initial treatment. The treatment regimens included ID (isazomib + dexamethasone), IRD (isazomib + lenalidomide + dexamethasone) and ICD (isazomib + cyclophosphamide + dexamethasone). The short-term curative effect and adverse reactions of relapsed/refractory patients and patients at initial onset were analyzed.Results:The overall response rate (ORR) of relapsed/refractory patients was 52.9% (9/17), of which 6 cases achieved complete remission (CR), 2 cases achieved very good partial remission (VGPR) and 1 case achieved partial remission (PR). The ORR of refractory patients receiving bortezomib therapy was 40.0% (4/10). The ORR of patients at initial onset who could be evaluated the curative effect was 100.0% (14/14), including 9 cases of CR, 2 cases of VGPR and 3 cases of PR. After treatment, 2 patients (6.2%) had grade Ⅲ-Ⅳ adverse events (1 case of herpes zoster and 1 case of thrombocytopenia), and none of the patients had grade Ⅲ-Ⅳ peripheral neuropathy.Conclusion:Isazomib is effective and safe in the treatment of initially treated and relapsed/refractory multiple myeloma.

OBJECTIVE: To explore the genetic etiology in four patients with hyperbilirubinemia, and discuss the correlation between clinical characteristics and molecular basis. METHODS: The data of clinical manifestation and auxiliary examinations were collected. Genomic DNA of the four patients was extracted and analyzed by next-generation sequencing using the panel including genes involved in hereditary metabolic liver diseases. Suspected variants were verified by Sanger sequencing. RESULTS: All of the four patients were males with normal liver enzymes. It was revealed that all the patients had heterozygous variants, among which c.3011C>T, c.2443C>T and c.2556del were the variants which have not been reported previously. CONCLUSION All of the patients were diagnosed as Dubin-Johnson syndrome (DJS) caused by ABCC2 gene variants. The novel variants add to the spectrum of genetic variants of the disease. Because of the favorite prognosis, precise diagnosis can greatly reduce the psychological pressure of patients and avoid excessive treatments. At the same time, it could provide pertinent genetic counseling for the families.
DNA ; Female ; Heterozygote ; Humans ; Jaundice, Chronic Idiopathic/genetics* ; Male ; Multidrug Resistance-Associated Protein 2 ; Multidrug Resistance-Associated Proteins/genetics* ; Phenotype

OBJECTIVE: To explore the genetic etiology of three pedigrees with a gestational history of fetal renal anomalies. METHODS: Peripheral venous blood or skin samples were derived from the probands of the three pedigrees. Copy number variation sequencing (CNV-seq) was applied to detect alterations of genome CNVs. RESULTS: The patient from pedigree 1 and the fetuses from pedigrees 2 and 3 all carried a heterozygous 17q12 deletion, with the size ranging from 1.4 Mb to 1.48 Mb encompassing the HNF1B gene. CONCLUSION The diagnosis of 17q12 microdeletion may be difficult during fetal period for its variable phenotypes. Alterations of chromosomal copy numbers need to be excluded in such patients.
Chromosome Deletion ; Chromosomes, Human, Pair 17 ; genetics ; DNA Copy Number Variations ; Fetus ; Genetic Testing ; Hepatocyte Nuclear Factor 1-beta ; genetics ; Humans ; Pedigree ; Phenotype

To investigate the possibility of microsurgical anastomosis of artery, vein and lymphat-ic vessel under 3-dimension screen without eyepiece. Methods During March, 2019, 2 cases (48 and 62 years old) were operated for breast reconstruction, chest wall deformity modified, and axillary scar contracture release, under 3-dimension screen without eyepiece.Deep epigastric artery perforators (artery and vein) dissections were carried on, and microsurgical anastomosis of artery, vein and lymphatic vessel were finished. Coupler was used to do the end-to-end anastomosis of veins (2.5 mm), interrupted suture end-to-end anastomosis with 9-0 nylon for artery (2.0 mm). Reverse arm lymphatic dynamic fluorescence methylene blue tracer under Near Infrared Imaging was used to test the func-tion of lymphatic system. The ends of 2 dominant drainage lymphatic vessels was found in the released axillary area (0.2 mm and 0.3 mm, respectively), and were anastomosis to the vein (0.5 mm) of lateral chest lymphatic tissue.Im-mediate methylene blue tracer under near infrared imaging was used to confirm the patency of lymphatic vessels-veins anastomosis and follow-up post operation. Flap were monitored use HHD. Results Two patients recovered well, and the flaps survived completely with appreciated appearances. The lymphedema of the arms were getting better, the peripheral diameter was reduced by about 2.0 cm compared with that before operation. Conclusion The technique of microsurgical anastomosis of artery, vein and lymphatic vessel without eyepiece under 3-dimension screen is possi-ble and safe.

OBJECTIVE: To explore the genetic etiology for a pedigree affected with progressive familial intrahepatic cholestasis (PFIC). METHODS: Target sequence capture and next generation sequencing (NGS) were applied for the proband. PCR and Sanger sequencing were used to verify the suspected mutation in his sister with similar symptoms and his parents. RESULTS: The proband and his sister manifested after birth with symptoms including jaundice, pruritus and developmental retardation. NGS has identified compound heterozygous mutations of ABCB11 gene, which encodes bile salt export pump protein (BSEP), namely c.2494C>T (p.Arg832Cys) and c.3223C>T (p.Gln1075*), in the proband, which were inherited from his father and mother respectively. His sister carried the same compound mutations. CONCLUSION Based on the phenotype and genetic testing, the patients were diagnosed as PFIC2 caused by mutation of the ABCB11 gene. The c.3223C>T is a novel nonsense mutation which may cause premature termination of translation. Above results have enriched the spectrum of ABCB11 mutations and provided new evidence for the molecular basis of PFIC, which also facilitated genetic counseling for this pedigree.
ATP Binding Cassette Transporter, Subfamily B, Member 11 ; genetics ; ATP-Binding Cassette Transporters ; Cholestasis, Intrahepatic ; genetics ; Female ; Genetic Testing ; Humans ; Male ; Mutation ; Pedigree ; Phenotype