A cold saponification method for determination of 5 lutein stereoisomers in dairy products by high performance liquid chromatography ( HPLC ) was developed. Samples were cold-saponified at room temperature and extracted by n-hexane/petroleum/dichloromethane ( 2: 2: 1 , V/V/V ) . Then 5 lutein stereoisomers were separated on a YMC C30 column with gradient elution using methanol/methyl tert-butyl ether as the mobile phase, and data were acquired by a photodiode array detector at wavelength of 445 nm. The calibration curve was linear in the range of 0 . 127-5 . 082 mg/L with correlation coefficient of 0 . 9999 , and the recoveries were from 96 . 7% to 102 . 2% with the RSDs in the range of 4 . 1%-5 . 4% ( n=6 ) . The limit of detection was 0 . 010 μg/g ( S/N=3 ) , and the limit of quantification was 0 . 030 μg/g ( S/N=10 ) . By presenting results of good accuracy, precision and sensitivity, this method validates its suitability for routine analysis of 5 lutein stereoisomers in dairy products.

Objective To explore the effects of follow-up management based on structural family therapy on self efficacy and quality of life in discharged puerpera. Methods A total of 124 hospitalized puerpera with childbirth of the Affiliated Hospital of Medical School of Ningbo University were selected from October 2015 to October 2016. All of them were divided into observation group and control group, 62 cases in each group. Puerpera of control group were treated with conventional nursing. On the basis of the conventional nursing, puerpera of observation group adopted follow-up management based on structural family therapy. We compared the scores of breast feeding knowledge questionnaire, the rate of exclusive breastfeeding and the quality of life with the Exercise of Self-Care Agency Scale (ESCA) and the quality of life index scale of puerpera in two groups. Results The mean score of breast feeding knowledge questionnaire of puerpera in the observation group was higher than that in the control group with a significant difference (P<0.05). The rate of exclusive breastfeeding of puerpera in the observation group was 93.54% higher than that in the control group (74.19%) with a significant difference (P<0.05). There was a statistically significant difference in the total score of self efficacy of puerpera between the observation group and the control group [(143.4±14.0) vs. (109.6±11.6)] (P< 0.05). The total score of quality of life of puerpera in the observation group was significantly higher than that in the control group [(8.8±1.3) vs. (5.6±1.1)](P< 0.05). Conclusions The follow-up management based on structural family therapy is favourable to improve the self efficacy on nursing and quality of life of puerpera so as to increase the rate of breast feeding.