Objective: To establish an experimental animal model of chromic alcoholic fatty liver.Methods: Fifty three Wister rats were divided into four groups: control group and model groups(lower dose ethanol group?medium dose ethanol group and higher dose ethanol group).The rats in model groups were induced by ethanol intragastric infusion of 40%(v/v)ethanol orally [7g/(kg?d) for lower group?8 g/(kg?d) for medium group and 9 g/(kg?d) for higher group],two times a day for the first 4 weeks.Ethanol intragastric infusion of 50%(v/v)ethanol orally [8 g/(kg?d) for lower group,9 g/(kg?d) for medium group and 10 g/(kg?d) for higher group],two times a day for the second 4 weeks.Then,the liver was examined by Ultrasonography.The pathologic changes were observed and the amount of ALT?AST??-GT in serum were detected at the end of the eighth week.Results: At the end of eighth week,serum AST and ?-GT levels were increased significantly in model groups.The typical fatty liver pathologic changes of chronic alcoholism were observed in rats in medium and higher dose model groups.Conclusions: Experimental model of chronic alcoholic fatty liver can be established in rats with ethanol intragastric infusion.

Objective:To study the effect of Lycium Barbarum Pclysaccharide(LBP)on the expression of hepatocyte cytochrome P4502E1(CYP2E1) in rat model of alcoholic fatty liver(AFL).Methods: The AFL rats models were established by ethanol intragastric infusion in ten weeks,then,5% and 10% LBP and 10% GSH intragastric infusions were adopted to treat those AFL model rats.Liver pathologic changes were observed and CYP2E1 gene and protein expressions as well as the contents of malondialdehyde(MDA) and glutathione(GSH) in liver were detected and compared with those in the control group,treatment groups and abstinence treatment group.Results: Fatty degeneration in liver recovered normally in the LBP-treated groups.LBP could inhibit the expressions of CYP2E1 gene and protein,reduce the content of MDA,and increase the content of GSH.The curative effect of LBP was of no significant difference compared with GSH,but had more effect than abstinence treatment.Conclusion: LBP can prevent AFL though inhibiting the hepatocyte CYP2E1 expression markedly.

Objective:To study the effect of Lycium Barbarum Polysaccharide(LBP) on the activity and gene expression of hepatocyte cytochrome P4503A(CYP3A) in rat model with alcoholic fatty liver (AFL). Methods:The AFL rats models were established by ethanol intragastric infusion for ten weeks. Then,those AFL model rats were treated with 5% and 10% LBP and 10% GSH intragastric infusion. Liver pathologic changes were observed and the activity of CYP3A and its gene expression as well as the contents of malondialdehyde(MDA) and glutathione(GSH) in liver were detected and compared among the control group,treatment group and abstinence treatment group. Results:Fatty degeneration in liver recovered normally in the LBP treatment groups. LBP could inhibit the expression of CYP3A gene,reduce the activity of CYP3A and the content of MDA,and increase the content of GSH. The curative effect of LBP was not significantly different from that of GSH,but greater than that of abstinence treatment. Conclusion:LBP can prevent AFL through markedly inhibiting the hepatocyte CYP3A activity and gene expression.

Objective:To evaluate the effect of hydrotalcid combined with Tibolone,a compound with the effects of estrogen,progestogen and androgen on the menopause patients with bile-reflux gastritis and 24-hour intragastric bile.Methods:Nineteen menopause women with bile-reflux gastritis proven by gastroscope and 24-hour intragastric bile monitoring were divided into 2 groups at random, which were treated with hydtotalcid 1.0g three times a day and the same dose of hydrotalcid combined with Tibolone 2.5mg one time a day.The severity of the symptoms including heartburn,epigastric distention,nausea and bile vomiting was investigated before and after 2,4 weeks treatment.Twenty-four hour intragastrie bile monitoring was also reexamined.Results:The severity of all the symptoms in the two groups decreased greatly after 2 weeks treatment.After 4 weeks treatment,the severity of all the symptoms decreased further.The total fraction time of bile reflux,the time of the longest bile reflux in two groups decreased greatly after treatment ( P

Objective:To investigate the effect of tibolone on the symptoms of functional dyspepsia in menopausal women.Methods:143 menopausal patients with functional dyspepsia were divided into 3 groups at random.They were treated with tibolone or cisapride or both drugs respectively for 4 weeks.The scores of symptoms including epigastric pain and fullness,abdominal distention after meal,early satiety and belching were recorded before and after 2 or 4 weeks' treatment.From the 3 guoups we drew out 20 patients at random.The gastric emptying was measured before and after 4 weeks' treatment respectively.Results:The symptoms of epigastric pain and fullness,abdominal distention after meal were improved after 2,4 weeks' treatment in three groups.Either tibolone or tibolone combined with cisapride remarkably relieved early satiety and belching,but no response was observed in the treatment of cisapride.The combination of tibolone and cisapride did not show better effect than tibolone treatment alone.The 30-min gastric emptying rates of all groups were significantly improved ( P 0.05).Conclusion:Compared with cisapride,tibolone can increase gastric emptying and show better relief for the symptoms of functional dyspepsia in menopausal patients.

Objective To investigate the expression of long non-coding RNA FRAPT(lncRNA FRAPT) in triple-negative breast cancer(TNBC) cells and analyze its effects on proliferation,cell cycle and drug resistance of TNBC cells.Methods Using scRNASeqDB and TCGA on-line bioinformatics database,the expression of lncRNA FRAPT in TNBC and its correlation with prognosis were detected;TNBC cell line MDA-MB-231 was transfected with si-lncRNA FRAPT and si-Ctrl(negative control) by using Lipofectamine~(TM) 2000 respectively,of which the proliferation and cell cycle changes after transfection were detected by SRB test and flow cytometry,and the drug resistance to chemotherapy drug paclitaxel(PTX)was detected.Results The expression of lncRNA FRAPT was up-regulated in TNBC tissues and cells,and its high expression was positively correlated with the poor prognosis of TNBC patients(Hazard Ratio=1.66,P=0.047).Silencing lncRNA FRAPT significantly inhibited the proliferation of TNBC cells,with the cell cycle arrested in G1 phase,while increased the sensitivity of TNBC cells to PTX.Conclusion LncRNA FRAPT was highly expressed in TNBC and related to tumor prognosis-Silencing lncRNA FRAPT inhibited proliferation and cell cycle of TNBC,and increased the sensitivity to chemotherapy drug PTX.

Breast cancer related lymphedema is the accumulation of interstitial fluid due to the lymphatic fluid load exceeds its transport ability as a consequence of the lesion of lymphatic structure.It mainly presents as swelling of the affected limb,a feeling of heaviness or tightness in the limb,aching and restricted limb motion.Its incidence is related to surgery,chemical therapy,obesity,hypertention as well as the alteration of particular genes.To prevent the occurrence and exacerbation of lymphedema,patients should avoid the overuse of the affected limb.While limb exercise step by step can solve part of this problem,but not all,doctor should also take both the patterns of exercise and risk factors that patients expose into consideration,then make a individualized exercise plan,evaluate the condition precisely,in order to achieve the best treatment purposes.

Objective To investigate the relationship between helicobacter pylori(HP)eradication and curative effect of gastroe-sophageal reflux disease(GERD) .Methods Two hundred and fifty four patients with GERD were prospectively recruited for endo-scope and acid reflux evaluation .According to the results of gastroscope ,the eligible patients were divided into non-erosive reflux disease (NERD) and erosive esophagitis (EE) group ,and each divided into HP positive and HP negative group ,the HP positive group were randomly divided into the anti HP treatment group and not .These patients were assigned to rabeprazole triple therapy (the anti HP treatment group) or rabeprazole(20 mg ,twice per day) single(other groups) for 10 d ,then rabeprazole (20mg ,twice per day) was given to all patients until two months .Then all patients underwent acid reflux evaluation ,EE group underwent endos-copy ,and all patients underwent a 13 C urea breath test 4 weeks after cessation of rabeprazole to determine HP status .Results One hundred and seventy nine GERD patients were included in the study(NERD group 119 cases ,including anti HP treatment group 47 cases ,not anti HP treatment group 32 cases ,HP negative group 40 cases ;EE group 60 cases ,including anti HP treatment group 24 cases ,not anti HP treatment group 15 cases ,HP negative group 21 cases) .HP was eradicated in 76 .6% of the NERD anti HP treatment group and in 75 .0% of the EE anti HP treatment group .Overall ,there is no difference between the anti HP treatment group and other groups on these aspects ,including symptom、esophagitis and acid reflux improvement .Conclusion There are no significant correlation between HP eradication and GERD .

Objective To observe the effectiveness of lactobacillus acidophilus combined with electroacupuncture on gastrointestinal function in patients with traumatic brain injury. Methods A total of 167 patients with traumatic brain injury treated in our hospital from June 2013 to December 2016 were randomly divided into combining treatment group (n=84) and lactobacillus acidophilus group (n=83). The lactobacillus acidophilus group was given routine treatment and lactobacillus acidophilus. The combining group was given the routine treatment, electroacupuncture treatment and lactobacillus acidophilus. Data of gastronintestinal function, infection, pH of gastric juice, serum two amine oxidase (DAO) and D-lacticacid level were compared between two groups. Results Compared with lactobacillus acidophilus group, the incidence rates of reflux (χ2=7.948), vomit (χ2=7.514), diarrhea (χ2=4.521), abdominal distension (χ2=5.968), pulmonary infection (χ2=4.521) and enterogenic infection (χ2=4.531) were significantly decreased in the combining treatment group (P<0.05). After 20-day treatment, pH value of gastric juice (t=8.125) was significantly increased, the serum DAO value (t=6.383) and D-lacticacid level (t=6.745) were decreased, in combining treatment group than those of lactobacillus acidophilus group (P<0.05). Conclusion Lactobacillus acidophilus combined with electroacupuncture can improve the gastrointestinal function, stimulate gastric acid secretion and decrease serum levels of DAO and D-lacticacid.

With the emergence of new technologies such as brain imaging, bio-sensing, human-computer interaction, cloud computing and large data, the treatment of traumatic brain injury has entered a new era. However, it must be clearly recognized that the central nervous system (CNS) trauma remains a worldwide medical problem. Based on the existing medical knowledge, it is important for promoting CNS trauma treatment levels including nerve trauma standardized treatment, professional management team, multi modal monitoring and data analysis, actively participating in domestic and international multi-center clinical research. Medical workers should dare to explore and innovate, keep up with the pace of the times, and truly achieve the overall improvement of nerve trauma.