With the understanding of the function mechanism of RNAi,it has been widely applied in various fields.RNAi is a powerful tool to study gene functions and provides a new technical method for the specific gene treatment as well.The further development of RNAi technique opens up a new way for tumor gene treatment.

Objective To evaluate the effect of nasal continuous positive airway pressure (nCPAP) in pediatric patients with respiratory failure after liver transplantation.Method A prospective? randomized controlled clinical trial was conducted during June 1st 2013 to June lst2015 in 71 pediatric patients with respiratory failure after liver transplantation.A total of 66 patients completed the trial and 5 patients quitted.Conventional oxygen therapy group included 35 cases and nCPAP group included 31 cases.The vital sign,blood-gas analysis,intra-abdominal pressure and prognosis were compared between the two groups.Result After nCPAP treatment,the heart rate,respiratory rate,and PCO2 decreased at 4 and 24 h (P＜0.05),oxygenation index was improved at 24 and 48 h (P＜0.05),and intra-abdominal pressure decreased at 48 h (P＜0.05).nCPAP showed better outcome than conventional oxygen therapy (P＜0.05).The incidence of refractory atelectasis,intra-abdominal hypertension,and epilepsy was higher in treatment failure population.Conclusion nCPAP is more suitable for pediatric patients with respiratory failure after liver transplantation,especially for those accompanied with intra-abdominal hypertension.

Objective To compare the efficacy and adverse reactions between floxuridine (FUDR) and 5-fluorouracil (5-Fu) treatment of gestational trophoblastic tumor.Methods 111 trophoblastic tumor patients of Ⅱ-Ⅲ stage including 62 cases of in vasive mole and 49 cases of choriocarcinoma were analyzed retrospectinely.The patients were divided into FUDR+KSM group (55 cases) and 5-Fu+KSM group (56 cases).FUDR was according to 23 mg/kg·d phleb instillation,keeping 8 hours,KSM was according to 6 μg/kg·d phleb instillation,keeping 4 hours.5-Fu was according to 26 mg/kg·d phleb instillation,keeping 8 hours.KSM was according to 6 μg/kg·d phleb instillation,keeping 4 hours.Both the two programs were 8 days.Results The cure rate was 98 ％ (54/55) in FUDR+DSM group,and that was 96 ％ (54/56) in 5-Fu+KSM group.The incidence rates of phlebophlogosis,dental ulcer and alopecie in the FUDR+KSM group were obviously lower than those in 5-Fu+KSM group [29.1％ (16/55) vs 85.7 ％ (48/56),54.5 ％ (30/55) vs 98.2 ％(55/56),21.8 ％ (12/55) vs 89.3 ％ (50/56),all P ＜ 0.05].Both the FUDR+KSM group and the 5-Fu+KSM group bad significant differences at phlebophlogosis dental ulcer and alopecie in Ⅲ stages.Conclusion The curative effect of the FUDR is aimilar with the 5-Fu,but the drug consumption of the FUDR is less.The incidence rates of dental ulcer,alopecie,and phlebophlogosis are less,but myelosuppression and alvi profluvium are rcscmble between the two sets.

OBJECTIVE To discuss the disinfection effect of Laoken KDSJ-Y multi-purpose air disinfecting machine using in the newborn hospital ward for air sterilization.METHODS Standard sample were carried on separately before starting,the starting 60 minutes later,the engine off latter 60 minutes and 120 minutes,after raising and the bacteria number were calculated.RESULTS Dynamic disinfection in room temperature 30-31℃,humidity 68-72%.Room population 10.7,and does not limit in the undertake transfer of personnel situation,starting disinfects 60 minutes to be able to achieveⅡ The kind of disinfection ambient air contains the fungus quantity standard;After before starting disinfection,and engine off,colony quantity continues to increase,cannot achieve toⅡ The kind of disinfection ambient air including the fungus quantity request after 120 minutes,must starting disinfect once more.CONCLUSIONS Laoken KDSJ-Y multi-purpose air disinfecting machine under some person of conditions the dynamic disinfection to be possible in the control air the bacterial content,conforms to the newborn hospital ward 2 kind of ambient air disinfection sanitation standard,suits the promotion.

OBJECTIVE To discuss and focus on the hospital infection management in the secondary care and over level hospitals,and the effective control of nosocomial infection.METHODS For the problems and hidden drisks,were targeted to take a scientific and effective prevention and control measures to strengthen the management of the details.RESULTS By changing the concept of process-oriented monitoring,training and scientific management the nosocomial infection effectively controlled.CONCLUSIONS Only the standardized management and operations,security checks and routine testing can be a real focus on hospital infection management.

Objective To observe the expression and relevance of human papillomavirus L1 (HPVL1)capsid protein and tumor suppressor gene p16~4A in cervical lesions.Methods The expression of HPVL1 capsid protein and p16INK4A in liquid-based cytology specimens and organization from 210 cases infected with HPV virus were detected by Max-vision immunohistochemistry method.Results In different grade of liquidbased cytology specimens,the positive rates of HPVL1 capsid protein had statistic difference (x 2 =70.50,P ＜ 0.005).The rate in LSIL couples was 68 ％ (34/50),which was the highest in all couples.The positive rates of tumor suppressor gene p16INK4A were increased gradually.In SCC couples,the positive rates was 100 ％(30/30),which was the hishest.In LISL couples,the rate of HPVL1+ p16INK4A-was 32 ％ (16/50),which was the hishest.In SCC couples,the rate of HPVL1-p16INK4A+ was 100 ％ (30/30),which was the highest.In orgnizational specimens,the positive rates of HPVL1 capsid protein in cervical intraepithelial neoplasia had statistic difference (x2 =54.37,P ＜ 0.005).The rate in CIN Ⅰ group was 60.4 ％ (32/53),which was the highest.The positive rates of tumor suppressor gene p16 were increased gradually.In cervical cancer group,the positive rate was 100 ％ (28/28),which was the highest.In CIN Ⅰ group,the rate of HPVL1+ p16INK4A-was 45.3 ％ (24/53),which was the highest.In cervical cancer couples,the rate of HPVL1 (-) p16INK4A(+) was 100 ％ (28/28),which was the highest.Conclusion Detection on the expression of HPVL1 capsid protein and tumor suppressor gene p16INK4A can diverse different levels of cervical lesions and separate cases from aggravated or self-healing,to avoid over-treatment or misdiagnose.

ObjectiveTo screen for proteins related to invasive and metastatic potential of ovarian cancer.MethodsThe nude mice were intraperitoneally injected with the human ovarian cancer 3AO cell line.The metastatic tumors were isolated and cultured in vitro.The cell lines were established from tumors and transplanted intraperitoneally for several passages.Invasive potential of cell lines was evaluated by cell adhesion and matrigel invasion assays.Lysates from different cell lines with high or low invasive potential were analyzed by surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS)and protein-chip array technology.The proteomic fingerprint patterns were compared. ResultsThe subclones 3AOF3 and 3AOF4 were isolated from repeated passaging in vivo and vitro,and showed higher invasive capacity than 3AO cells.By comparing their proteomic fingerprints,9 distinct proteins (3494,3831,8121,8293,9123,9332,10 184,15 436 and 17 134 at m/z value) were found,which were expressed only in 3AO cells.On the contrary,1 distinct protein (4360 at m/z value) was expressed only in 3AO F3-4 cells,and 2 distinct proteins (1842 and 3145 at m/z value) were expressed only in 3AO F2-4 cells.Additionally,6 distinct proteins (1236,5476,5649,6575,6897 and 7016 at m/z value) and a mass cluster (13 794 and 14 027 at m/z value)were expressed in all 3AO and 3AOF1-4 cells,while the amount of protein was different.ConclusionBy comparing the proteomic fingerprints of isogenic ovarian cancer cells with different invasive potential,a group of proteins are identified which are correlated with high invasive and metastatic potential.

Objective To analyze the clinicopathologic features,prognosis and influencing factors of invasive cervical adenocarcinoma.Methods Clinical and pathologic data of 96 cases of invasive cervical adenocarcinoma and 100 cases of squamous carcinoma of the cervix treated in Shanxi Cancer Hospital from years of 1998 to 2007 were retrospectively analyzed.Results The morbidity of cervical adenocarcinoma was showing the arise and low-age trend.The overall 5-year survival rate was 60.4 ％,The overall survival rates of patients with stage Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 82.9 ％,51.5 ％,13.3 ％ and 0,respectively.Ocervical adenocarcinoma had many prognosis-related factors:clinical stage,lymmphatic spread,vascular invasion,volume of tumor,histologic grade.Multivariate analysis showed that clinical stage,lymph nodal metastasis and vascular invasion were independent prognosis-related factors.However,whether ovary was reserved,it was irrelative to prognosis.Conclusion The morbidity of cervical adenocarcinoma is showing the arise and low-age trend.Earlier diagnosis is extremely important for improving the survival rate of cervical adenocarcinoma,the reserved ovary doesn't influence the overall 5-year survival rate for young women with early cervical adenocarcinoma.

Objective To assess the change of plasma adrenomedullin(ADM)and endothelin(ET)in patients with essential hypertensin. Methods Concentrations of plasma ADM and ET were measured in 18 healthy control subjects and 52 patients with essential hypertension, 36 patients of which had no target organ damage, while 16 patients of which were complicated with renal impair. Results The concentrations of ADM and ET were significantly higher in patients with renal impair than in patients without organ damage. Plasma concentrations of ADM and ET were strongly positively correlated with blood urea nitrogen levels and serum creatinine levels. A strong inverse correlation between GFR and plasma concentrations of ADM and ET was found. Conclusion The increase of plasma ET and ADM may have an intimate relation to the renal function. ADM and ET may play some important role in the pathophysiologic makeup of hypertension and hypertension with renal dysfunction. ADM may act against excessive vasoconstrictor ET increased in essential hypertension and may be part of a compensatory mechanism to offset further development of renal impair.

Objective To compare the effects of sedation induced with midazolam and propofol on blood pressure(BP), heart rate(HR) and heart rate variability (HRV) during operation on lower extremity preformed under continuous epidural anesthesia in patients with the type Ⅱ diabetes. Methods Forty patients with type Ⅱ diabetes scheduled for lower limb surgery were randomized to receive either midazolam (group M n - 20) for propofol (group P n = 20) for sedation during operation, all patients were classified as ASA physical status Ⅱ or Ⅲ . The patients were unpremeditated. Epidural catheter was inserted at L2-3 or L3-4 . The local anesthetic used was 2% lidocaine. MAP,HR,HRV and SpO2 were monitored during anesthesia and operation. After onset of epidural analgesia a loading dose of midazolam(0.03 mg?kg-1 ) or propofol (0.7 mg?kg-1 ) was given i.v. followed by intermittent i.v. boluses of 1 /4-1 /2 loading dose to maintain the depth of sedation at OAA/S score of 3 (respond to name outer if called repeatedly) . Changes in HRV before and during sedation were recorded and compared between the two groups. Results The demographic data including sex, age and body weight were comparable between the two groups. LF and TP decreased significantly during sedation as compared with baseline in both groups ( P