Objective To develop an indirect ELISA method for quantitative detection of antibodies against pertussis toxin(PT), filamentous hemagglutinin(FHA) and pertussis adhesin(PRN) in serum of mice, and verify and preliminarily apply the method in order to provide reference for the optimization of production process of pertussis vaccine.Methods Enzyme-labeled plates were coated with PT, FHA and PRN antigens respectively, and the pertussis anti-mouse serum standard and mouse serum samples were added. Using goat anti-mouse IgG labeled with HRP as the enzyme-labeled secondary antibody,the contents of PT, FHA and PRN antibodies in serum of mice were quantitatively determined by the indication degree of TMB color development. The specificity, linear range, accuracy and precision of the developed indirect ELISA detection method were verified. The serum of mice immunized with pertussis vaccine and qualified internal reference products was detected by the developed method.Results The results of the pertussis anti-mouse serum standard and mouse serum samples detected by PT, FHA and PRN antibody ELISA were positive, while the other samples were negative. The linear range of ELISA for PT antibody was 0. 005 3-0. 17 U/mL, the recovery rate was 90. 91%-104. 77%, the RSD of reproducibility verification was 6. 31%, and the RSD among personnel was 6. 40%. The ELISA for FHA antibody had the linear range of 0. 022 3-1. 43 U/mL, the recovery of 95. 84%-102. 18%, the RSD of reproducibility verification of 9. 02%, and the RSD among personnel of 5. 79%. The ELISA for PRN antibody showed the linear range of 0. 009 4-0. 3 U/mL, the recovery rate of 86. 27%-100. 22%, the RSD of reproducibility verification of 6. 94%, and the RSD among personnel of 8. 90%. The detection results of mouse serum immunized with pertussis vaccine and qualified internal reference products by the developed method showed that, the PT antibody levels of six groups of samples were significantly higher than those of the internal reference products, while the FHA and PRN antibody levels were equivalent to those of the internal reference products.Conclusion The developed ELISA methods have strong specificity, good linear correlation(R2> 0. 99), high accuracy and good precision, and can be used for quantitative detection of pertussis(PT, FHA, PRN) antibodies in mouse serum, providing a reference for the optimization of pertussis vaccine production process.

China ; Humans ; Infant, Newborn ; Intensive Care, Neonatal ; Respiration, Artificial ; Respiratory Care Units

Galectin-9 is a novel eosinophil chemoattractant,which belong to Galectin family.Galectin-9exhibits a variety of biological functions,such as cell differentiation,maturation,adhesion and aggregation,cell chemotaxis,activity,and cell death.Galectin-9 may play important roles in the development of acute inflammation as well as chronic inflammation associated with allergies,autoimmune diseases,infectious processes.This review summarizes the advances in Galectin-9 study.

Hypoxia-inducible factor-I is the critical factor existed in mammal and human cells which plays an important role in maintaining the oxygen balance. Many hypoxia-responding genes can be activated by H1F-1. So, the relationship between HIF-land matrix matalloproteinases/stromal-derived factor-I/special CXC chemokin receptor/angiogenesis related factors is the hot spot in research of the tumor invasion and me-tastasis.

Objective To investigate the significance of procalcitonin(PCT),C-reactive protein (CRP)and WBC counts in predicting the postoperative jnfections in patients with bone fractures.Methods Clinical data of 49 patients with open fractures were retrospectively analyzed,and the patients were grouped into the infeeted(n=24)and non.infected(n=25).PCT,CRP and WBC values were detected 1,4,7and 10 days after the operations,and the results were compared with those taken from the healthy controls.Areas under the receiver operating characteristic(ROC)curves were used to determine the value in predicting postoperative infections.Results PCT,CRP and WBC values in the infected group and the noninfected group were higher than those in health controls(F=19.84,57.71 and 35.44,P＜0.01).PCT,CRP and WBC values in the infected group were higher than those in the non-infected group 4.7 and 10 days after the operations(tPCT=7.31,7.74 and 4.59;tCRP=2.23,5.75 and 8.01;tWBC=2.34,2.51 and 4.07,P＜0.05).ROC curves suggested that PCT value was more sensitive and had higher specificity than CRP and WBC values in the diagnosis of postoperative infections.Conclusions PCT,CRP and WBC values arise in bone fracture patients with postoperative infections.Detection of PCT has higher sensitivity and specificity.which can be used in early diagnosis of postoperative infections.

Cervical intervertebral disc replacement effectively tackles limited motion range of the neck and long-term adjacent degeneration acceleration following traditional anterior cervical fusion and fixation. This paper reviews history of cervical intervertebral disc replacement,current prosthesis types,related indications and complications,and further investigates the feasibility of minimally invasive replacement.

Objective To control the hazardous effects of excessive arsenic in drinking water on human health. Methods The experiment on the removal of excessive arsenic from drinking water by electric absorb technology (EST) was performed. Results The pilot experiment and experiment in situ showed that, after treatment by EST, the contents of arsenic in source water(0.06-0.33 mg/L) decreased to the levels of

Cardiomyopathies ; Heart Ventricles ; Humans

Objective To explore the effects of sports training on endothelin-1 ( ET-1 ) and endothelin-1 receptor (ETR) and its subtypes in the myocardium in rats. Methods After sports training, levels of endothelin-1 were measured using radioimmunoassay, and changes in ETAR and ETBR levels by radioligand binding assay. Results The endothelin-1 level in myocardial cells was significantly reduced ( P