Objective To investigate effect of dicoumarol on the expression of serum tumor necrosis factor-α( TNF-α) in patients with acute cerebral infarction.Methods Eighty cases patients with acute cerebral infarction were admitted and randomly divided into two groups by digital draw:control group and observation group, 40 cases in each group.The control group were given conventional therapy for treatment of acute cerebral infarction, and observation group were given dicoumarol thrombolysis on the basis of control group.The serum TNF-αlevel and neurologic impairment score were compared between two groups pre-and post-treatment.Results After treatment of 6 h, the serum TNF-αlevel in observation group was significantly lower than that in control group (P<0.05).After treatment of 24 h, 48 h and 30 d, there were no significant differences of serum TNF-αlevels between two groups.After treatment of 5 d, 14 d and 28 d, the neurological deficit scores in observation group were significantly higher than those in control group (P<0.05).Conclusion Dicoumarol could effectively reduce TNF-αlevels in serum and extent of cerebral cell damage in patients.

Objective:to study alexithymia of long-term survivors of cancers. Method:to compare 36 long-term survivors of cancers and 45 normal controls with TAS (Toronto alexithymia scale) and SCL-90.Result:there was no significant difference between the two groups in total score of SCL-90. But the survivors had significant higher score in TAS than controls.Conclusion:although long-term survivors are similar in symptom level to normal control, they still have obvious alexithymia.

Objective To review the study of noninvasive imaging methods for evaluating liver fibrosis.Meth- ods The current literatures on the use of the ultrasonography,CT and MRI for the evaluation of liver fibrosis were reviewed.The principles,applications and advancement of each imaging methods were described and summarized re- spectively.The features of the newly developed imaging techniques were also discussed.Results In addition to the morphologic information,the imaging examinations can also provide functional information about the circulation sta- tus,diffusion and metabolism features of liver.The potential diagnostic value of MR elastography for liver fibrosis has been addressed.Conclusion The imaging examinations,especially the functional MRI techniques,are reliable noninvasive alternatives for the evaluation of hepatic fibrosis,with bright potentiality for clinical application.

Objective To investigate the effects of exercise on behavior and serum cortisol levels in depression caused by chronic,unpredictable,mild stress(CUMS).Methods Forty-eight male Sprague-Dawley rats were randomly divided into 3 groups:A,B and C.Each group was then divided into 3 subgroups:a blank control subgroup,a model subgroup,and an intervention subgroup.CUMS such as unpredictable forced swimming in cold water,electric stimulation of the animals'feet and shaking of the cage was used to establish a model of depression.Swimming exercise was administered as a therapeutic intervention.In group A it was administered while the animals were under stress,while in groups B and C,exercise was administered before and after the depression model had been established.Open-field test and fluid consumption data were recorded during the whole procedure.At the end of the experiment,radioimmunity assays were used to measure the levels of serum cortisol.Results Rats exposed to CUMS showed reduced water consumption;a preference for sucrose solutions;and decreased squares crossed,less rearing and increased bowel movement in open-field test.All of these changes were mitigated by the exercise performed while the animals were under stress,but not by exercise before or after the depression model had been established.Rats exposed to CUMS showed significant increases in serum cortisol,which was mitigated by exercise prior to and during stress.Conclusion Different exercise routines have different effects on behavior and serum cortisol levels in depressive rats under chronic unpredictable mild stress.Exercise has cumulative effects in decreasing the serum level of cortisol,but has only immediate effects on the behavior of depressive rats.

Objective To determine plasma resistin level in patients with traumatic brain injury (TBI) and evaluate its correlations with outcome and inflammatory reaction. Methods Fiftyfour patients with moderate TBI, 71 patients with severe TBI and 40 healthy controls were enrolled in this study. Plasma samples were obtained from the healthy controls on physical examination and from the TBI patients on admission. Enzyme-linked immunosorbent assay ( ELISA) was used to determine the plasma resistin concentrations. Results Twenty patients (37.0% ) and 53 patients (74.6% ) with moderate and severe TBI suffered from an unfavorable outcome (defined as GOS score for 1-3 points) three months after TBI respectively. Plasma resistin levels in the patients with moderate and severe TBI were substantially higher than that in the healthy controls ((21. 9 ± 8. 4) ng/ml and (29. 2 ± 9. 6) ng/ml vs (9. 3 ± 2.6) ng/ml, both P ＜0. 01] by using covariance analysis. By using the multivariate linear regression analysis, plasma C-reactive protein level (t =2.212,P =0.035; t =2. 274,P =0. 014) and GCS scores (t =3. 120,P =0.007; t=3.986,P=0.003) were associated with the plasma resistin levels. Logistic regression analysis selected plasma resistin level as an independent predictor for 3-month unfavorable outcome of the patients with moderate and severe TBI (odds ratio = 1. 124, 95% CI = 1. 040-1. 221, P = 0.011; odds ratio = 1. 145, 95% CI = 1. 044-1. 232, P = 0. 009). A receiver operating characteristic curve identified cutoff levels of plasma resistin (22.4 ng/ml and 30.5 ng/ml) that predicted 3-month unfavorable outcome of moderate and severe TBI patients with the high sensitivity (70. 0% and 79. 2% ) and specificity (70.6% and 72.2% ) ( area under curve = 0.719, 95% CI = 0.642-0.829, P = 0.000;area under curve =0.735, 95% CI =0. 671-0. 893, P = 0.000). Conclusions Plasma resistin level is increased after TBI and may be involved in inflammatory response of brain injury. Clinical detection of this indicator can help early determine the prognosis of the TBI patients.

0.05); The result of FCM indicates that the cells transfected with p16 changed in cell cycle distribution ; proportion of G 2 M phase was increased, while that of S phase was decreased.Conclusions:Introduction of p16 gene into low expression human lung adenocarcinoma cell line Anip973 alters the radiation effect in a way which perhaps was correlated with the inhibition of sublethal damage repair and the cell cycle distribution change, but not in the high expression cell line AGZY83a.

In this paper,a number of valuable experiences focusing on teaching idea,cultivation of high-quality teachers and deepening of teaching content and method reform were summarized through exploring the construction and practice of the national excellent course-medical genetics. Aim of the ex-ploration is provide references for the construction and application of national excellent course and its sus-tainable development.

Objective To detect the proteus and analyze the drug-resistance in intensive care unit.Methods From Jan 2005 to Dec 2006,proteus was detected by Disc diffusion test(K-B method)to study the antimicrobial resistance in ICU and general ward,and compared the drug-resistance.Results The isolating rate of proteus was 80.9% in sputum,and proteus mirabilis accouted for 80.4%.The resistance rates to ciprofloxacin,amikacin,cefotaxim,ceftazidime,cefmetazole and piperacillin/Tazobactam were between 64%~77%,and 32%~41% in imipenem and cefoperazone/sulbactam.Compared with general ward,the antibiotic resistant rate existed significant difference.And the antibiotic resistant rate of other type proteus had significant difference between ICU and general ward.Conclusion Properly detecting Proteus in time is imporrant to use antibiotic and to avoid nosocomial outbreak infections by Proteus.

Objective We have examined tumor suppressor gene p53 from N- methyl - N- nitrosourea (MNU ) induced the col - orectal tumors in rats. Methods PCR, SSCP and DNA sequence analysis. Results In colorectal tumors induced by MNU, the mobil- ity shifts of p53 gene mutation were observed, p53 gene mutation was a deletion at third position C of codon 244 in exon7. Conclu- sion The present study suggested that MNU induces rat p53 mutations in the colorectal tumors.

AIM: In order to search carrier material and better tissue culture method, the morphology and structure of the cultured cat corneal endothelium was observed. METHODS: The cat corneal endothelial cells were seeded on the Descemet's membrane of the dehydrated swine corneal stroma, and then cultured in the medium with epidermal growth factor and laminin for 7 days. The morphology and structure of reconstructed tissue was tested by the inverted microscope and the scanning electron microscope. RESULTS: As shown by the morphological observations, the cultured cat corneal endothelial cells formed an integrated membrane, and attached to the Descemet's membrane, which was similar to the nature tissue. The cells connected tightly to each other, and some of them arranged in hexagon approximately. CONCLUSION: The cat corneal endothelial cells could be rebuilt on the carrier of the dehydrated swine corneal stroma successfully, which is similar to the nature tissue.