1.Therapeutic effect of syringin on adjuvant arthritis in rats and its mechanisms.
Yuanyuan SONG ; Yuan LI ; Hongquan ZHANG
Acta Pharmaceutica Sinica 2010;45(8):1006-11
This study is to investigate the therapeutic effect of syringin on adjuvant arthritis (AA) in rats and its mechanisms. Complete Freund's adjuvant (FCA) was used to induce AA in rats. Secondary paw swelling of AA rats was measured with volume meter. Pain response and polyarthritis index were scored. Meanwhile, splenic lymphocyte proliferation response induced by concanavalin A (ConA) or lipopolysaccharide (LPS) was examined with MTT assay. IL-2 production of splenic lymphocytes and IL-1 beta, TNF-alpha production of peritoneal macrophage (PM phi) were estimated by enzyme linked immunosorbent assay (ELISA). The secondary inflammation of AA rats appeared on the 14th day after injection of FCA. Syringin and tripterygium glycosides (TG) were given by intragastric administration for 16 days from the 14th day. Treatment of AA rats with syringin and TG from the 22th day significantly attenuated the secondary hind paw swelling, as well as relieved the pain response and the polyarthritic symptoms of the whole body as compared with that of the AA model group. The suppressed lymphocyte proliferation and IL-2 production of splenic lymphocytes in AA rats were reversed by treatment with syringin. Meanwhile, syringin remarkably down-regulated IL-1 beta, TNF-alpha productions from PM phi. These results indicate that anti-inflammatory effects of syringin on AA rats are mediated by modulating the immune function of abnormal cells and the balance of cytokines.
2.Effect of Echinacoside on immune function and mitochondrial DNA relative content of aging mice
Yuan LI ; Yuanyuan SONG ; Hongquan ZHANG
Chinese Pharmacological Bulletin 1986;0(06):-
Aim To study the effect of Echinacoside on immune function and the hepatocyte mitochondrial DNA relative content of experimentally aging model mice. Methods Mice were given sc 10%D-galactose 10 ml?kg-1,once daily for 8 weeks to establish the model of aging mice. Vitamin E group ,and ECH groups were given ig Vitamin E 40 mg?kg-1 and ECH 20,40,60 mg?kg-1,respectively at one time. The interleukin-2(IL-2) and interleukin-6(IL-6) content in peripheral blood was measured by ELISA method.Phagocytosis of peritoneal macrophages was tested by neutral red test. Proliferation and transformation of spleen lymphocytes induced by ConA were determined by MTT method. The mitochondrial DNA relative content of liver cells was measured by the means of SDS alkali decomposition from liver.Results The IL-2 content,phagocytosis of peritoneal macrophages and proliferation of spleen lymphocytes were obviously decreased in the aging model group as compared with those in the normal control group.The IL-6 content and mitochondrial DNA relative content of liver cells were significantly increased. ECH 20,40,60 mg?kg-1 and Vitamin E 40 mg?kg-1 could improve the IL-2 content,phagocytosis of peritoneal macrophages and transformation of spleen lymphocytes,and reduce the IL-6 content and mitochondrial DNA relative content of liver cells. Conclusion ECH can enhance immune function and reduce the mitochondrial DNA relative content of liver cells,which may be one of the mechanisms of anti-aging.
3.Effects of Different Scaffolds and Cultures on Growth of Preadipocytes
Jianfeng FEI ; Hongquan SONG ; Yang SUN ; Rui ZHANG ; Fengqin ZHANG
Chinese Journal of Medical Aesthetics and Cosmetology 2010;16(4):252-255
Objective To culture preadipocytes in vitro and to study the cell compatibility of PLGA scaffolds,collagen scarfolds and hyaluronic acid-based scaffolds and tO choose the optimal seeding method.Methods The preadipocytes from human abdominal adipose tissue were isolated and cultured in enzyme-digesting method.The generation of human preadipocytes was planted on PLGA scaffolds,collagen scaffolds and hyaluronic acid-based scaffolds.and the cell compatibility was observed by MTT method.The seeding efficiency of human preadipocytes on scaffolds.human preadipocytes were seeded to hyaluronic acid-based scaffolds by static culture and stirred culture.Results Compared compatibility of preadipocyte with three different scaffolds,there was great difference between hyaluronic acid-based scaffolds and PLGA scaffolds.Difference also existed between hyaluronic acid-based scaffolds and collagen scaffolds that were different from PLGA scaffolds.Among them,hyaluronic acid-based scaffolds was the best.Conclusion Hyaluronic acid iS a better scaffolds material for adipose tissue engineering compared with PLGA and collagen.The seeding efficiency of stirred culture is higher than static culture,which is an optimal method for cell seeding tO 3-D scaffolds.
4.A Progress on the Effects of Resveratrol on Tumor Cells
Junjun HAN ; Yuming ZHANG ; Xuefeng PAN ; Song XIE ; Fei DUAN ; Hongquan LI
World Science and Technology-Modernization of Traditional Chinese Medicine 2016;18(12):2176-2181
Resveratrol is a natural polyphenolic substance with a variety of bioactivities,such as anti-oxidation,anti-tumor and apoptosis induction.This paper summarized the recent research progress of resveratrol induced cancerous cell apoptosis,including human cervical cancer HeLa cells,leukemia cells,hepatoma cells and gastric cancer cells,and analyzed the molecular mechanism behind resveratrol-induced cell apoptosis in tumor.In addition,resveratrol was investigated by blocking the cell cycle,regulating the expressions of related genes and proteins and mitochondria-induced apoptosis pathways as a potential application of cancer drugs to clinical researches.
5.Research progress on the association between neutrophil extracellular traps and oral squamous cell carcinoma
Shengfeng YANG ; Xin GU ; Rui ZHANG ; Hongquan SONG
Journal of Prevention and Treatment for Stomatological Diseases 2024;32(11):894-900
Neutrophil extracellular traps(NETs)are fibrous web-like structures composed of decondensed chromatin and granular proteins released by neutrophils,with the ability to capture and kill bacteria.Pathogens,such as bacteria and viruses,can trigger the formation of NETs via NETosis,a type of programmed cell death that has two distinct forms:suicidal NETosis and vital NETosis.Numerous studies have found that NETs interact with immune cells in the tumor microenvironment,where they activate macrophages,promote immunosuppressive effects of myeloid-derived suppressor cells,and coat the tumor surface to prevent cytotoxic effects of CD8+T cells and natural killer cells.Recent research has identified a substantial presence of NETs in oral squamous cell carcinoma(OSCC)tissues,indicating a complex re-lationship between NETs and OSCC development.Depending on the phenotype of neutrophils,NETs may exhibit pro-tu-mor or anti-tumor effects.For instance,NETs derived from N1-type neutrophils may exert anti-tumor effects,while TGF-β-induced NETs derived from N2-type neutrophils may exert pro-carcinogenic activity,thereby contributing to the devel-opment of oral squamous metaplasia.Furthermore,NETs likely play a role in OSCC metastasis by capturing circulating tumor cells and inducing a hypercoagulable state,thereby facilitating tumor-related thrombus formation and hematoge-nous metastasis.The involvement of NETs in the occurrence and progression of OSCC opens new avenues for anti-tumor therapy and prognostication.Inhibiting NET formation can significantly suppress the development of chemotherapy-in-duced drug resistance and reduce the risk of thrombosis in OSCC patients,thereby inhibiting tumor metastasis.Current-ly,multiple prognostic models based on NET-related genes have been constructed and validated for head and neck squa-mous cell carcinoma,indicating the potential clinical value of NETs.However,the association between NETs and OSCC treatment is still unclear,necessitating further research on its underlying mechanisms and feasibility.This article attempts to review the relationship between NETs and OSCC,aiming to provide novel perspectives for OSCC treatment.
6.Autophagy in tumorigenesis and cancer therapy
Xin WANG ; Haiping SONG ; Ye WANG ; Hongquan SUN ; Xuezhen MA
Journal of International Oncology 2018;45(12):743-746
Autophagy is a crucial physiological process for cellular self-renewing and homeostasis,which plays a dual role in both tumorigenesis and anti-tumor treatment.It plays an antineoplastic role by maintaining genomic stability,as well as a protective role in tumorgenesis and progression.Autophagy improves the effectiveness of anti-tumor therapy,meanwhile,it involves in resistance of tumor cells to chemotherapy and irradiation.Using relevant autophagy regulators in different backgrounds is expected to become a new strategy for improving sensitivity of tumor cells to anti-tumor treatment.
7.Molecular classification of head and neck squamous cell carcinoma based on hypoxia-related genes and clinical significance of STC2
ZHU Jianing ; WANG Tiantian ; ZHANG Rui ; SONG Hongquan
Journal of Prevention and Treatment for Stomatological Diseases 2025;33(5):345-358
Objective :
To construct a molecular classification system for head and neck squamous cell carcinoma (HNSCC) utilizing hypoxia-related gene (HAG) expression profiles, and to comprehensively examine the clinicopathological significance and biological functions of the hypoxia gene stanniocalcin 2 (STC2) in HNSCC.
Methods :
Transcriptomic data and clinical information of 546 HNSCC samples were obtained from The Cancer Genome Atlas (TCGA) database, and based on the expression profiles of 200 HRGs, HNSCC was classified subclasses using non-negative matrix factorization (NMF). HNSCC was classified into three subclasses (C1, C2, and C3), and the molecular characteristics and prognostic differences of the subclasses were assessed by comparing the tumor mutation load, functional enrichment analysis, drug sensitivity, and clinical features among the subclasses. LASSO-Cox regression was used to screen prognosis-related genes and construct prognostic models. Using oral squamous cell carcinoma (OSCC)-related data in the TCGA database, we analyzed the expression differences of STC2 in OSCC and control samples, and detected the mRNA and protein expression of STC2 in oral squamous carcinoma samples using qRT-PCR and immunohistochemistry. We knocked down STC2 in CAL-27 cells and verified the knockdown efficiency by qRT-PCR and Western blot. CCK-8 assay and cell scratch assay were used to assess the effect of STC2 on cell proliferation and migration ability.
Results:
Based on HRGs expression profiles, HNSCC was categorized into three subclasses (C1, C2, and C3). Subclass C1 had moderate hypoxic activity and good prognosis; subclass C2 had the highest hypoxic activity, poor prognosis, and poor sensitivity to CTLA-4 inhibitors (P<0.05); subclass C3 had the lowest hypoxic activity and moderate prognosis, and STC2 belonged to subclass C3. The frequency of cyclin-dependent kinase inhibitor 2A (CDKN2A), phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA), and tumor protein p53 (TP 53) mutations was higher in HNSCC. C1 genomic gain and deletion burden were significantly higher than C3 subclass (P<0.05) and C2 genomic gain than C3 subclass (P<0.05). The C2 subclass was significantly enriched in hypoxia-associated pathways, such as glycine metabolism and base excision repair (P<0.05). The C1, C2, and C3 subclasses were significantly positively correlated in terms of sex (male) (Cramer’s V=0.15), radiation exposure (Cramer’s V=0.12), medication (Cramer’s V=0.18), and pathological grading (G1/G2) (Cramer’s V=0.25) (P<0.05). Nine prognosis-related genes were screened by LASSO-Cox regression, among which high expression of STC2 was positively correlated with poorer overall survival (OS) in HNSCC patients (P<0.01). Bioinformatics analysis showed that STC2 mRNA expression was higher in OSCC than in normal controls (P<0.05). qRT-PCR and immunohistochemistry confirmed that both mRNA and protein expression of STC2 were significantly upregulated in OSCC tissues and cells (P<0.01). In vitro experiments showed that STC2 expression was knocked down to approximately 80% in CAL-27 cells (P<0.001), and the STC2 knockdown group had a reduced value-added rate (P<0.001) and a reduced percentage of scratch closure (P<0.05) compared with the control group.
Conclusion
We successfully constructed a molecular typing system for HNSCC based on the expression profiles of HRGs and categorized HNSCC into three subclasses with significant prognostic differences, among which the C2 subclass had the highest hypoxic activity and the poorest prognosis. STC2 was highly expressed in HNSCC and suggested a poor prognosis, demonstrating that it may be a potential target for HNSCC treatment.
8.A model for predicting the probability of poor outcome at 3 months after intravenous thrombolysis for elderly patients with acute cerebral infarction
Wei XU ; Huiping LI ; Zhen WANG ; Guohua HE ; Jue HU ; Kangping SONG ; Yangping TONG ; Fangyi LI ; Hongquan GUO ; Xinfeng LIU
Chinese Journal of Geriatrics 2022;41(11):1303-1309
Objective:To explore independent predictors for poor outcome at 3 months in elderly patients with acute cerebral infarction(ACI)treated with intravenous thrombolysis(IVT), and to develop a nomogram-based predictive model.Methods:This was a retrospective cohort study.Clinical, laboratory and imaging data of 346 elderly patients with ACI treated with IVT from January 2016 to April 2021 in our hospital were collected.Poor outcome was defined as a modified Rankin Scale(mRS)score >2 at 3 months after the stroke.Logistic regression analysis was used to screen for independent factors predicting poor outcome in elderly ACI patients treated with IVT, and a corresponding nomogram model was developed using the R software.The ROC curve, calibration plots and decision curve analysis were used to evaluate discrimination, calibration and clinical application value of the nomogram model.Results:Among 346 candidates, 109 developed a poor outcome, representing a rate of 31.5%.Logistic regression analysis showed that symptomatic hemorrhagic transformation( OR=15.647, 95% CI: 8.913-27.454), stroke severity(moderate stroke, OR=3.322, 95% CI: 1.414-7.811; moderate-severe stroke, OR=8.169, 95% CI: 4.102-16.258; severe stroke, OR=9.653, 95% CI: 5.440-17.121), stroke-associated pneumonia( OR=2.239, 95% CI: 1.134-4.420), and heart failure( OR=2.758, 95% CI: 1.424-5.336)were independent predictors for poor outcome at 3 months in elderly ACI patients treated with intravenous thrombolysis(all P<0.05). With the area under curve(AUC-ROC)value at 0.85(95% CI: 0.80-0.89), the nomogram model, which was composed of the above four predictors, demonstrated good discrimination.On the calibration plot, the mean absolute error was 0.020, indicating that the model had good calibration.Decision curve analysis revealed that the model had good clinical application value. Conclusions:The nomogram model composed of symptomatic hemorrhagic transformation, stroke severity, stroke-associated pneumonia and heart failure may predict poor outcome at 3 months in elderly ACI patients treated with IVT, with high prediction accuracy and high clinical application value.
9.Pediatric reference intervals for plasma and whole blood procalcitonin of in China: a multicenter research
Zhan MA ; Fangzhen WU ; Jiangtao MA ; Yunsheng CHEN ; Guixia LI ; Jinbo LIU ; Hongbing CHEN ; Huiming YE ; Xingyan BIAN ; Dapeng CHEN ; Jiangwei KE ; Haiou YANG ; Lijuan MA ; Qiuhui PAN ; Hongquan LUO ; Xushan CAI ; Yun XIE ; Wenqi SONG ; Lei ZHANG ; Hong ZHANG
Chinese Journal of Laboratory Medicine 2022;45(6):581-588
Objective:To establish the biology reference interval (RI) of peripheral blood procalcitonin (PCT) for children between 3 days and 6 years old in China.Methods:Totally 3 353 reference individuals with apparent health or no specific diseases were recruited in 18 hospitals throughout the country during October 2020 to May 2021. Reference individuals were divided into four groups: 3-28 days, 29 days - 1 year, 1-3 years and 4-6 years. Vein blood or capillary blood were collected by percutaneous puncture from every reference individual. The PCT level in serum and the capillary whole blood were assayed by Roche Cobas e601 and Norman NRM411-S7 immunoanalyzer. Outliers were deleted and 95th percentiles of every group were provided as RIs. Man-Whitney U test or Kruskal-Wallis test were used performed to assess the difference among different gender, age or method groups. Results:The difference of PCT distribution between male and female is not statistically significant, but the difference between serum and capillary whole blood is statistically significant. The differences between age groups are significant too. For Roche e601, serum PCT RI of 3-28 days group is <0.23 μg/L, 29 days - 6 years are <0.11 μg/L. For NRM411, Serum PCT RI of 3-28 days group is <0.21 μg/L, 29 days - 1 year: <0.09 μg/L, 1 - 6 years: <0.10 μg/L. For whole blood PCT, RI of 3-28 days group is <0.26 μg/L, 29 days - 6 years is <0.15 μg/L.Conclusions:Serum and capillary whole blood PCT have different RIs, however, capillary whole blood PCT testing is valuable in pediatric application. Children in 3-28 days show higher PCT levels than other age group. To establish the RIs and understand the differences among different groups are essential for the interpretation and clinical application of peripheral blood PCT testing results.
10.Analysis of risk factors of frozen shoulder
Li XIANG ; Hongquan SONG ; Honggen DU ; Junlong XIONG ; Yaoyu JIN ; Zukang QIAO
China Modern Doctor 2024;62(10):10-12
Objective To explore the risk factors of frozen shoulder,and to provide the basis for the prevention of frozen shoulder.Methods A total of 114 patients with frozen shoulder who were hospitalized in the First Affiliated Hospital of Zhejiang Chinese Medical University from January 2020 to August 2022 were included in case group,and 114 physical examination patients with no history of frozen shoulder were included in control group.The clinical data of two groups were collected and the risk factors of frozen shoulder were analyzed by multivariate Logistic regression.Results There were statistically significant differences in cervical radiculopathy,type 2 diabetes mellitus,hepatitis,chronic non-atrophic gastritis,lumbar disc herniation,osteoporosis and thyroid sarcoidosis between two groups(P<0.05).Multivariate Logistic analysis showed that cervical radiculopathy(OR=6.114,95%CI:1.458-25.642)and type 2 diabetes mellitus(OR=24.069,95%CI:4.023-144.007)were independent risk factors for the onset of frozen shoulder.Conclusion Patients with type 2 diabetes mellitus and cervical radiculopathy have a higher risk of frozen shoulder,and should pay attention to early prevention.