7;there were 32 b ands in tumor group. ③SDS PAGE showed that the number of protein bands with relative molecular mass of 77 000,50 000-52 000,38 000-30 000 increased in the tumour group. Conclusion: In the saliva of periodontitis indivauals there are more basic proteins,the relative molecular mass of the prot ein in the saliva of patients with tumor is different from that of health contro ls.

Objective To examine the differences in the structure of brain white matter among deficit schizophrenia, nondeficit schizophrenia and healthy controls by using voxel-based morphometry (VBM). Methods Ten deficit schizophrenic patients, eleven nondeficit patients and fifteen healthy comparison subjects participated in the study. All the subjects were scanned by GE Twin Speed 1.5T MRI system. Whole brain, voxel-wise analyses of regional white matter volume were conducted by the VBM toolbox on the Matlab7.6 and SPM5. t -test was then used for the comparison between groups. Results Compared to the healthy controls, nondeficit schizophrenic patients significantly decreased the density of gray matter in the frontal, parietal, temporal, occipital lobe and basal ganglia , while the deficit patients showed the characteristically broad and significant decreasion in the frontal lobe, including left medial frontal gyrus, bilateral inferior frontal gyrus, left middle frontal gyrus, and left orbital gyrus (Cluster ≥ 30 mm3, P<0.01). Moreover, deficit patients showed the decreasion in the temporal cortex and the limbic lobe (right insula). Relative to the nondeficit schizophrenic patients, deficit patients had significant regional gray matter decreases in the left medial frontal gyrus, bilateral inferior frontal gyrus, right precentral gyrus, and right superior temporal gyrus (Cluster ≥ 30 mm3, P<0.01). Conclusion Structural heterogeneity in schizophrenia may relate to specific patterns of gray matter density reductions in deficit and nondeficit patient. However the two subtype of schizophremia patients share a common prefrontal-temperal pattern of structural brain alterations.

Objective To investigate the prevalence and risk factors of non?suicidal self injury among middle school students. Methods Data were collected from 2 140 middle school students with the mean age of 13.92 by stratified sampling method. All students were evaluated with Beck Depression Scale (BDI),Barratt Im?pulsiveness Scale ( BIS ) , Adolescent Self?Rating Life Events Check List ( ASLEC ) , Adolescents Health related Risk Behaviors Inventory ( AHRBI ) , Chinese version of Family Adaptability and Cohesion Evaluation Scales ( FACES II?CV) ,and self?made investigate questionnaire. Results The prevalence of non?suicidal self injury a?mong local middle school students was 23.2%,total 495 adolescents were endorsed non?suicidal self injury,and 49.7% were girls.The results of in multivariable logistic regression showed the main risk factors for non?suicidal self injury among adolescents were being female,depressive symptoms,impulsiveness,negative life events and health re?lated risk behaviors. Conclusion Non?suicidal self injury is high among Chinese adolescent in the city of Dujian?gyan,and it is necessary to take effective interventions.

Objective: To select highly metastatic cells from human salivary gland mucoepidermoid carcinoma cell clone Mc3. Methods: In situ transplantation of Mc3 cells into submandibular gland of nude mice, in situ transplantation of Mc3 induced lung metastasized tumor tissue among nude mice and cell culture were employed to obtain the wanted cells. Morphological observation, cell growth analysis, flow cytometry, chromosome staining, clonogenic assay and artificial metastasis test in nude mice were used to characterize the cells. Results: Lung metastasis was observed in 3 out of 10 nude mice after 4 cycles of in situ transplantation of Mc3 cell induced lung metastasized tumor tissue. Epidermoid cells with similar morphology to Mc3 were obtained through cell culture and the cells were named M3SP4. M3SP4 cell induced lung metastatic foci were histologicaly proved to be mucoepidermoid carcinoma. Subdiploid karyotype with human chromosome morphology was observed in M3SP4 and Mc3 cells. The population doubling time (h) of M3SP4 and Mc3 cells was 23.9 and 25.9, the percentage of S phase cells in cell cycle 26.8 and 15.3, clonogenecity (%) 54.6 and 30.2, respectively. The artificial lung metastatic potential of M3SP4 cells was 35% higher than that of Mc3 in nude mice. Conclusion: M3SP4 cells are of human mucoepidermoid carcinoma with higher metastatic potential than Mc3. In situ transplantation of mucoepidermoid carcinoma cells or lung metastasized tumor tissue may maintain the metastatic potential of the cells.

Objctive: To study the inhibitory effects of homoharringtonine(HHT) or methoxsalen (8-MOP) or in combinnation on humen highly metastatic mucoepidermoid carcinoma Mc3 cells. Methods: Mc3 cells were exposed to the drugs or their combination at various concentrations. The inhibitory effects were tested with MTT assay. Results: The IC 50 values of HHT and 8-MOP were 79.43 ng/ml and 5 980 ng/ml respectively, HHT at the doses of 1-320 ng/ml combined with 8-MOP at 800, 4 000 and 20 000 ng/ml the CI 50 values were 0.76?0.33 and 0.15 respectively.Conclution: The data indicate that combination of HHT with 8-MOP is synergic in the inhibition of Mc3 cell growth.

Objective To understand the prevalence and risk factors of non-suicidal self-injury in middle school students.Methods 1312 middle school students of Pengzhou and Santai were selected to fill in a Risky Behavior Questionnaire for Adoluscents (RBQ-A),Family Environment Scale ( FES ),Center for Epidemiological Survey,Depression Scale (CES-D),Adolescent Self-Rating Life Events Check List (ASLEC),Social Support Scale for Adolescents (SSSA) and self-administered questionnaire.In all the research subjects,1288 were qualified for the study in April 2011 before the risk factors for non-suicidal self-injury were identified by logistic regression.Results In 1288 middle school students,22.67％ had a history of non-suicidal self-injury,with 22.70％ in boys and 22.64％ in girls.63.36％ of students had injured themselves through variouslyways,more seen in boys (26.88％) than in girls (11.36％ ) who cut or burnt themselves.The scores of ASLEC and CES-D in non-suicidal self-injury group appeared higher than that in the control group and the score of SSSA was found higher in the control group.The main risk factors for non-suicidal self-injuries were family conflict,depressive emotion,negative life events and receiving less social support.Conclusion The prevalence of non-suicidal self-injury among middle school students in Pengzhou was high,whicn called for more attention.

OBJECTIVETo establish the model of bone mesenchymal stem cell-derived smooth muscle cells (BMSC-SMCs) and investigate the role of BMSC-SMCs in the development and progression of artherosclerosis.

METHODSBMSCs were isolated from the femoral bone of SD rats by adherent tissue culture method, and vascular smooth muscle cells (VSMCs) were obtained from the thoracic aorta. The differentiation of BMSCs into BMSC-SMCs was induced in the conditioned medium. The specific markers of BMSCs and BMSC-SMCs were identified by immunofluorescence (IF) staining. After treatment with 80 mg/L oxidative low-density lipoprotein (ox-LDL) for 72 h, the growth characteristics of BMSC-SMCs and VSMCs were observed. Flow cytometry was applied to analyze the cell cycle of BMSC-SMCs and VSMCs.

RESULTSBMCS-SMCs transformed into foam cells after treatment with ox-LDL, which was more obvious in comparison with VSMCs. The growth curve of BMSC-SMCs and VSMCs presented with an S-shape pattern with the cell doubling time of 20 and 32 h, which was reduced to 15 and 28 h after treatment with 80 mg/L ox-LDL, respectively. Flow cytometry showed that exposure to 80 mg/L ox-LDL significantly increased G(0)/G(1) and decreased S and G(2)/M phase cells in both BMSC-SMCs (P<0.01, n=3) and VSMCs (P<0.05, n=3) in comparison with the control cells.

CONCLUSIONBMSC-SMC might be involved in the formation of fatty core and accelerate the development of atherosclerosis.

Animals ; Atherosclerosis ; etiology ; Bone Marrow Cells ; cytology ; Cell Differentiation ; physiology ; Cell Proliferation ; drug effects ; Cells, Cultured ; Female ; Foam Cells ; cytology ; Lipoproteins, LDL ; pharmacology ; Male ; Mesenchymal Stromal Cells ; cytology ; Muscle, Smooth, Vascular ; cytology ; Rats ; Rats, Sprague-Dawley

OBJECTIVEThis study was to analyze the mental health status of the children and adolescents from the areas struck by Wenchuan earthquake and to understand the factors that may have impact on their mental health after exposing to the earthquake.

METHODSSubjects of this survey were students (8 - 16 years old) from Chendu, Dujiangyan, Beichuan and Wenchuan districts which were the most severely affected areas in the earthquake. We analyzed the subjects' mental health conditions after Wenchuan earthquake by using a general questionnaire that was composed of the Children's Revised Impact of Event Scale (CRIES-13) and Depression Self-rating Scale for Children (DSRSC). Students are investigated 7341 case, 7387 case, 7395 case after 3, 6, 24 months. The data were analyzed with logistic regression model.

RESULTSThe CRIES-13 scores were (27.51 ± 12.26), (23.54 ± 12.79) and (21.35 ± 12.59) (F = 28.842, P ≤ 0.05) and the DSRSC scores were (11.79 ± 5.73), (10.94 ± 5.50) and (10.75 ± 5.27) (F = 17.084, P ≤ 0.05) 3, 6 and 24 months after the earthquake, respectively. The risk factors associated with depression 3, 6, 24 months after the earthquake were gender (female) (OR values were 2.14, 2.72, 2.31; 95%CI values were 1.01 - 3.03, 1.10 - 5.01, 1.02 - 4.58), age (less than 12 years old) (OR values were 1.97, 2.22, 1.93; 95%CI values were 1.43 - 3.17, 1.02 - 3.54, 1.32 - 3.27), having family member injury and death (OR values were 2.19, 2.45, 2.35; 95%CI values were 1.04-2.89, 1.16-2.08, 1.02 - 2.79), desperation (OR values were 2.24, 2.09, 2.16; 95%CI values were 1.00 - 2.54, 1.70 - 2.58, 1.00 - 4.56). The risk factors associated with PTSD 3, 6, 24 months after the earthquake were gender (OR values were 2.16, 2.94, 1.96; 95%CI values were 1.36 - 2.53, 1.36 - 2.53, 1.06 - 3.92), age (less than 12 years old) (OR values were 3.51, 2.62, 1.92; 95%CI values were 1.22 - 5.40, 1.14 - 4.93, 1.08 - 3.35), having teachers and schoolmates injury and death (OR values were 2.32, 2.48, 2.36; 95%CI values were 1.17 - 2.48, 1.30 - 2.72, 1.02 - 2.85), having family member injury and death (OR values were 2.73, 2.21, 1.85;95%CI values were 1.67 - 3.61, 1.04 - 3.15, 1.05 - 2.38), witnessing injury (OR values were 2.17, 2.36, 2.34; 95%CI values were 1.15 - 3.65, 1.17 - 2.67, 1.02 - 3.67), witnessing death (OR values were 1.98, 2.39, 1.71;95%CI values were 1.10 - 2.56, 1.14 - 3.70, 1.57 - 3.88), desperation (OR values were 2.02, 1.94, 2.02; 95%CI values were 1.67 - 2.35, 1.66 - 2.27, 1.82 - 2.26).

CONCLUSIONThe symptoms of PTSD and depression of young survivors from the earthquake-struck areas had gradually alleviated 3, 6, 24 months after the earthquake. Age (less than 12 years old), gender (female), having family member injury and death, witnessing injury and death, and desperation were the main risk factors that affected the children and adolescents mental health.

Adolescent ; Child ; China ; epidemiology ; Depression ; epidemiology ; etiology ; psychology ; Disasters ; Earthquakes ; Female ; Humans ; Male ; Risk Factors ; Stress Disorders, Post-Traumatic ; epidemiology ; etiology ; psychology ; Surveys and Questionnaires

Objective : To evaluate the clinical outcomes of all-ceramic crowns whose oral digital impressions were scanned by 3Shape Trios. Methods : All the impressions were scanned by Trios and all the crowns were fabricated according to the standard procedure. The restoration quality were evaluated using modified United States Public Health Service (USPHS) criteria one year after treatment. Restoration integrity、 marginal adaptation、enamel wear、 color satisfaction and gingival index (GI) of the prostheses were evaluated according to the modified USPHS criteria. Results: The clinical 1 year survival rate of 92 porcelain crowns fabricated by 3-shape Trios digital impression and made by CAD/CAM was 100%. The scored A rates of restoration integrity、 marginal adaptation、 enamel wear、 secondary caries、color satisfaction and gingival index of the prostheses were 100%、91.3%、 98%、95.6%、96.7% and 94.7% after 1 year, respectively. Conclusion The clinical outcomes of all-ceramic crowns whose oral digital impressions were scanned by 3Shape Trios and whose crowns were made by CAD-CAM are satisfying.

Objective : To evaluate the clinical outcomes of all-ceramic crowns whose oral digital impressions were scanned by 3Shape Trios. Methods : All the impressions were scanned by Trios and all the crowns were fabricated according to the standard procedure. The restoration quality were evaluated using modified United States Public Health Service (USPHS) criteria one year after treatment. Restoration integrity、 marginal adaptation、enamel wear、 color satisfaction and gingival index (GI) of the prostheses were evaluated according to the modified USPHS criteria. Results: The clinical 1 year survival rate of 92 porcelain crowns fabricated by 3-shape Trios digital impression and made by CAD/CAM was 100%. The scored A rates of restoration integrity、 marginal adaptation、 enamel wear、 secondary caries、color satisfaction and gingival index of the prostheses were 100%、91.3%、 98%、95.6%、96.7% and 94.7% after 1 year, respectively. Conclusion The clinical outcomes of all-ceramic crowns whose oral digital impressions were scanned by 3Shape Trios and whose crowns were made by CAD-CAM are satisfying.