Objective:To investigate the effects of Yizhi Oral Solution on protein and RNA synthesis of brain tissue of mice. Methods: The radioactive intensities of 3H leucine in brain protein and 3H uridine in RNA of brain tissue of young mice were observed by the methods of incorporation in vivo and group control observation.Results: The radioaetive intensity of 3H leucine of Yizhi Oral Solution group was higher than that of cycloheximide pathological model group ( P

In order to observe the febrifugal response of needling "Chang Qiang Point", and analyse its mechanism lumbar spinal cord of rabbits were damaged in the expermental study. The experimental findings were as follows: 1. It was found that cAMP content in csf was striking increased, when leukocytic pyrogen fever of rabbits was significant. During needling "Chang Qiang Point" of febrile rabbits, however, febrile response was markedly inhibited(at 60 min~?T℃ was 0.23?0.07 and TRI4 0.61?0.03). There was significant difference(P0.05). 3. After damaging lumbar spinal cord of rabbits, there were certain change of rectal temperature and the febrile response, but it had no effect on cAMP content in csf. The authors infered that the impulses from "Chang Qiang Point" transmiled Via spinal cord might be closely associated with reflex mechanism and the change of cAMP content might play an important role in the mechanism of febrifugal effect during needling "Chang Qiang Point" in febrile rabbits.

Objective To explore the role of ripply1 in zebrafish dorsal-ventral development .Methods Using ze-brafish whole-mount in situ hybridization to examine the ripply1 expression pattern in early embryo development .To analyse the expression pattern changes of dorsal-ventral marker genes at shield stage and the morphological changes at 24 hpf (hours post-fertilization) after overexpression of ripply1 by injecting synthetic mRNA at 1-cell stage.Using Tol2 transposon technology to obtain a ripply1 promoter driven GFP transgenic fish and to identify promoter region that recapitulates endoge -nous ripply1 expression pattern .Results The in situ hybridization results revealed that ripply1 specifically expresses in the future dorsal region at shield stage .Overexpression of ripply1 caused an enhanced expression of dorsal marker genes and a reduction of ventral marker genes .Embryos overexpressing ripply1 also showed severely dorsalized phenotype , with enlarged head, reduced ventral yolk extension , and shortened posterior trunk and tail regions , and the formation of a secondary trunk axis.Transgenic fish revealed the maternal expression of ripply1 and suggested that a 1.2 kb promoter-driven GFP is able to recapitulate the endogenous gene expression pattern .Conclusion ripply1 may participate in the early development of dor-sal-ventral axis in zebrafish embryo .

Objective To analyze the pressure source and effect of comprehensive intervention in type 2 diabetes mellitus patients. Methods 200 diabetes patients were randomized into the observation group and the control group with 100 cases in each group. Comprehensive intervention was introduced into the observation group and the control group underwent routine treatment. The pressure source was investigated after intervention by questionnaires and the results were compared. Results Fearing of serious complications bocame the most common cause of pressure. The scores of Self-rating Depression Scale(SDS)and Self-rating Anxiety Scale(SAS)were lower and the biochemical index was also better in the observation group than those in the control group as well as those before intervention in the observation group(P＜0.05).Conclusion The depression, anxiety status and biochemical index in patients with type 2 diabetes mellitus were effectively controled by corresponding and comprehensive intervention measures according to the sequence order of pressure source.

Objective: For preparing the reference material of alkaline phosphatase(ALP), the purification and properties of ALP from porcine kidney were studied. Methods: The ALP purification procedure included isolation microvillus of porcine kidney cortices by solubilization the membrance-binding enzymes with 1-butanol, precipitation with ammonium sulfate, chromatography on DEAE-Sephacel,ConA-Sepharose,Sephadex G-200 and a immuno-affinity column of anti-GGT antibodies. Results: The purified enzyme had a specific activity of 402 kU/g protein at 37℃and was almost free of contaminating enzymes. The apparent Michaelis constants was 1.35 mmol/L, and the optimum pH was 10.40. Conclusion: The kinetic properties of the preparation were very close to those of the enzyme present in the human serum, The product was suitable as enzyme preparation for producing the enzyme reference material.

The occurrence and development of solid tumor is a multi-factor,multi-step and multi-gene interactive process.The generation of micro vasculature of solid tumor,as well as the interaction between tumor and micro-environment,decides the tumor's biological characteristics of growth,recurrence,invasion and metastasis.Reasonable application of anti-angiogenesis therapy enables the normalization of tumor vascular system to enhance the efficiency of chemotherapy and radiation treatment and prolong survival time in patients.

Objective To investigate the efficacy of losartan on the improvement of heart function and its effect on uric acid in patients with chronic heart failure (CHF). Methods Eighty-five patients with CHF were divided into two groups randomly. Routine anti-CHF treatment included cardiotonic, diuresis, isosorbide dinitrate, beta-blockers. In losartan group(n=43), patients were given routine anti-CHF treatment and losartan. The patients in control group(n=42) were treated by routine anti-CHF treatment and Benazapril. The heart functional was evaluated. The content of serum uric acid, electrocardiogram, and Duppler ultransonography were measured before and after treatment respectively. Results The variance of heart function, left ventricle diastoic diameter (LVDD), left ventricle ejection fraction (LVEF) and cardiothotic ratio did not show statistical difference. However, they were improved sharply as compared with base-line condition in both groups after 3 months and 12 months treatment(P

AIM:To evaluate the effect of Huazhuo Jiedu Huoxue Recipe(Herba Agastaches,Herba Eupatorii,Rhizoma Pinelliae,Pericarpium Citri reticulatae,Poria,etc.) on plasma orphanin FQ(OFQ) and prostaglandins in the patients of endometriosis(EM) with pain.METHODS:The concentrations of plasma OFQ and prostaglandins were measured by radioimmunoassay before and after the treatment on the patients of EM with pain.29 patients of EM with severe pain(severe group),31 patients with slight pain(slight group) and 25 patients without pain(control group) were studied.RESULTS:The levels of OFQ、 PGF_(2a)、 PGE_2、 6-keto-PGF_(1a) and TXB_2 in plasma were significantly higher in patients of EM with pain than that of patients without pain(P

Objective To investigate the influence of Zengshiling oral liquid on gastric emptying,plasma motilin (MOT) and small intestine propulsion of young rat. Methods Experiment animal were divided into five groups randomly:control group,large dose of Zengshiling oral liquid group,normal dose of Zengshiling oral liquid group,small dose of Zengshiling oral liquid group and Motilium group. The rats were gavaged by 0.9% of NaCl and corresponding drug. 7 days later,gastric emptying was assayed by phenol red,plasma MOT levels and carbon powder propelling rate of small intestine were also assayed. Results The rate of gastric emptying of Zengshiling oral liquid groups were significantly higher than control group (P

64 cases of gallstone pancreatitis were treatedin our hospital in recent eight years. Early biliaryoperations were performed in 36 cases and good re-sults were got. Gallstone pancreatitis has the char-acteristics of cholelithiasis, the temporary damageof liver and the manifestations of pancreatitis, aswell. The purpose of the paper is to analyse anddiscuss diagnostic methods and treatment of gall-stone pancreatitis. It is important to perform theexaminations of ultrasound scanning and liverfunction in differential diagnoses. The necessity ofoperation is emphasized. It is also proposed thatbiliary operation be performed early as soon as theinflammation of pancreas is controlled effectively.