Objective:To study the expression of NF-?Bp65 and bcl-x L gene in pancreatic carcinoma and their clinical significance.Methods:The expressions of NF-?Bp65 and bcl-x L protein in 52 pancreatic carcinoma were studied by immunohistochemistry,and their relationship to clinical pathology were analyzed.Results:The positive rates of NF-?Bp65 protein and bcl-x L protein in pancreatic carcinoma were 63.5% and 73.1% respectively,and which were higher than those of normal pancreatic tissues.The expression of NF-?Bp65 was related to tumor diameter,TNM stage and lymph node metastasis,and had no relation with the extent of differentiation and type of pathology.There was positive correlation between NF-? Bp65 and bcl-x L expression.Conclusion:NF-? Bp65 may be an important oncoprotein,and may have positive regulation on bcl-x L,then promote tumorgenesis.

AIM: To study the dynamic levels of tumor necrosis factor alpha (TNF-?), interleukin-6 (IL-6) and gamma interferon (IFN-?) produced by human dendritic cells infected with Dengue virus. METHODS: Monocytes isolated from healthy human peripheral blood were incubated in medium with GM-CSF and IL-4 for more than 7 days. DCs were then collected and identified by scanning electron microscope, immunohistochemistry and lymphocytes stimulatory ability. Human dendritic cells (DC) were infected with Dengue-2 virus (DV-2) in vitro, culture supernatants were collected in different time postinfection (6 h, 12 h, 24 h, 48 h and 72 h), DV antigen in human dendritic cells were demonstrated by an indirect immunofluorescent assay (IFA), production of TNF-?, IL-6 and IFN-? in the culture supernatants were evaluated by ELISA. RESULTS: After 7 days, typical dendritic cells could be obtained. Virus antigen were detected in infected DC by IFA. Dengue virus induces TNF-? and IL-6 secretion from DC and does not induce IFN secretion. CONCLUSION: Human dendritic cells are target of dengue virus infection. TNF-?, IL-6 production from DC are increased with DV infection. Dendritic cells may play an important role in DV pathogenicity and immunity.

In order to probe into effect of stimulation of wind, cold and Sdampness to the cervical part on oxygen free radical and prostaglandins, 24 rabbits were randomly assigned to control group and wind - cold - Sdampness group, head - lowering group and combined model group, 6 rabbits in each group, and changes of superoxide dismutase (SOD),lipid hyperoxide (LPO), prostanglandin E2 (PGE2), 6 -keto -PGF1a in affected local region and blood were observed. Results indicated that the commonly - seen pathogenetic factors of cervical spondylopathy such as wind, cold , dampness and lowering head might cause aspetic inflammation of the cervical part and abnormality of SOD, LPO, PGE2 and 6 - keto - PGF1a in affected local tissue and blood, and abnormal metabolism of oxygen free radical and prostagnadins might be one of important mechanisms of cervical spondylopathy induced by wind, cold and dampness.

Acupuncture Points ; Acupuncture Therapy ; instrumentation ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Popliteal Cyst ; therapy

Objective Study for the security,effectiveness and the related recurrence factors of the elderly primary chronic gouty arthritis with 3 different therapies.Methods Eighty-seven patients in the Huzhou Third Hospital from 2004.3 to 2005.4 with primary chronic gouty arthritis were randomized into 3 groups for a 12-month-observation:31 in group A were given the general therapies;32 in group B and 24 in group C were given the hydroxychloroqine and colchicine respectively besides the general therapy.Results The average of acute attack after treatment in the 3 groups was lower than those before treatment.The levels of blood uric acid decreased significantly(P

Objection: To study the characteristics of shame of patients with neurosis. Method: 52 patients with nuclear neurosis were evaluated by SCL-90, Shame Scale, and Children Trauma Questionnaire. The control group (n=30) was evaluated just by Shame Scale. Results:The total score of Shame Scale and the subscores of each factor were all higher in neurotic patients than in control group (total score 69.0?20.4/48.7?10.2, personality 29.4?9.7/19.8?5.8, behavior 23.5?6.5/18.0?4.2, body 8.3?3.4/5.7?2.2, family 7.7?3.9/5.2?1.7, t=3.15~5.09,P=0.001or

OBJECTIVE To find out the clinical characteristics of nosocomial infections with Stenotrophomonas maltophilia(SMA) and investigate the disinfectant resistant gene of qacE△1,improve the diagnosis and decrease of nosocomial infection cases.METHODS Toally 165 strains of SMA were clinically isolated from 2004 to 2007.The gene of qacE△1 was analyzed by polymerase chain reation(PCR) and homology of the strains was analyzed by the method of by pulsed field gel electrophoresis(PFGE) genotype.RESULTS Susceptible factors were old age,seriousness of underlying disease,prolonged hospitalization and invasive operation with infection of SMA.The lower respiratory tract infection was mostly common with SMA,rate of which was 87.9%.Antibiotic sensitive rate more than 80% against SMA was minocycline,trimethoprim-sulfamethoxazole and levofloxacin.The positive rate of gene qacE△1 was 13.3% in 60 strains tested.Two genotypes in SMA strains from respiratory ICU(RICU) were with the same clone.This result proved that clone transmission occurred in RICU.CONCLUSIONS SMA is an important nosocomial infection pathogen.With the multi-drug resistance,the therapy of infection with SMA is very hard in clinic.As result of gene of qacE△1 and the same clone transmission,clinicians should play an important role of surveillance of effect of disinfection.

Cardiovascular disease has become the first cause of human death.Among the many risk factors in coronary heart disease,depression has been identified as an independent prognostic risk factors.In patients with coronary heart disease accompanied by severe depression,the onset of angina,quality of life deterioration and the mortality will increase by 3～5 times.However,the majority of antidepressant drugs have adverse effects on cardiovascular disease adverse drug reactions,making coronary heart disease patients with depression,did not receive antidepressant treatment due.So more researches to clarify the anti-depressant drugs,safety for cardiovascular disease are needed.

Biologic scaffold materials derived from decellularized tissues and organs have been successfully used in tissue engineering and regeneration medicine.Decellularization methodology varies according to the differences of the origin,tissue density,composition,geometric and biologic properties of the tissue.Optimal decellularization method should be developed on the basis of complete cell content removal with the preservation of extracellular matrix (ECM) integrity and bioactivity.In this article,an overview of decellularization methods and their effect upon acellular bracket structure and composition are presented.

Cardiac Catheterization ; methods ; Heart Defects, Congenital ; therapy ; Humans