The latest advances on target therapies of cancer were presented on the 44th Annual Meeting of American Society of Clinical Oncology(ASCO)in 2008.One of the most important results was the outstanding efficacy of Cetuximab in Metastatic Colorectal Cancer fIncao patients with k-ras"wild-type".Besides,detection of k-ras statuswouldmakeitpossibleforthetailoredtargettherapy of mCRC.Itwasestimatedthat k-ras gene status would become a predictive molecular marker for Cetuximab treatment in mCRC.

Antineoplastic Agents ; therapeutic use ; Benzamides ; Chemotherapy, Adjuvant ; Gastrointestinal Neoplasms ; drug therapy ; metabolism ; pathology ; surgery ; Gastrointestinal Stromal Tumors ; drug therapy ; metabolism ; pathology ; surgery ; Humans ; Imatinib Mesylate ; Indoles ; therapeutic use ; Mutation ; Neoplasm Staging ; Piperazines ; therapeutic use ; Proto-Oncogene Proteins c-kit ; metabolism ; Pyrimidines ; therapeutic use ; Pyrroles ; therapeutic use ; Receptor, Epidermal Growth Factor ; genetics ; metabolism ; Vascular Endothelial Growth Factor A ; metabolism

Hepatocellular carcinoma (HCC) is one of the most common malignant tumor with increasing incidence worldwid.Most of patients with HCC are diagnosed at a late stage.Threrfore,the prognosis of HCC patients is generally poor with a 5-year survival rate of 20% if withoutoperration. Screening strategies including α-fetoprotein (AFP) and ultrasound every 6 months in patients with liver cirrhosis,the major risk factor for HCC development, have been recommended to detect HCC at earlier stages amenable to effective treatment strateges.AFP, however,is a marker with poor sensitivity and specificity and the ultrasound is highly dependent on the operator's experience.Apart from AFP, lens culinaris agglutinin-reactive AFP and des-gamma carbexyprothrombin and several other biomarkers(e.g., glypican-3,human hepatocyte growth factor,and insulin-like growth factor) have been proposed as markers for HCC detection.In addition,with recently employed techniques,such as gene-expressing microarrays and proteomics,it is to be expected that new HCC-specific markers will become available in the near future.For all such proposed markers,however,the clinical usefulness has to be carefully evaluated and validated.

The opening rate, information update and menu of official WeChat in 11 Hangzhou municipal public hospitals were analyzed in order to understand their running, which showed that of the 11 public hospitals that opened their official WeChat,36. 36% opened their service account and 63. 64% opened their subscription ac-count. The information update frequency of WeChat varied and the online service function needed to be further deepened. Suggestions were put forward for the WeChat running in hospitals, such as normalizing its information push, deepening its online service, diversifying its communication between physicians and patients, and intensifying its popularization.

Objective:We try to examine the hypothesis that perceptual reversal is function of frontoparietal cortex. Methods:We employed block design in functional MRI, extracted event signal of endogenous reversal of Necker cube and compared with that of exogenous reversal. Results:Functional MRI revealed that spatial varieties were significant in bilateral superior and middle frontal lobe. Conclusion:Perceptual reversal may be the function of frontal cortex.

Objective To explore the relation between single umbilical artery with other malformations and pregnancy outcome,and to provide evidence for pregnancy counseling and diagnosis.Methods The outcomes of 92 fetuses with single umbilical artery diagnosed and delivered in Beijing Obstetrics and Gynecology Hospital Affiliated to Capital Medical University from September 2007 to July 2009 were followed up and retrospectively analyzed with Chi-square test.Results Among 25 292 pregnant women,92 cases were finally diagnosed as single umbilical artery giving the incidence of 0.36％,among which 57.6％ (53/92) of newborns survived without malformations,15.2％(14/92) survived with malformations and 27.2％ (25/92) died during perinatal period.There were 36 fetuses (39.1％,36/92) with single umbilical artery complicated with other malformations,among which 29 had single malformation (80.6 ％ ),and seven ( 19.4 ％ ) had multiple malformations.The incidence of heart defects was the highest among the complicated malformations,and then followed by central nervous system,digestive system,motor system and urinary system.Thirty-three fetuses with single umbilical artery accepted karyotype analysis.Chromosome abnormality was diagnosed in three subjects (9.1％,3/33).Twenty-three fetus (25.0％,23/92) associated with small for gestational age,and among which 18 fetuses (78.3％,18/23) died with a higher mortality than that (4.0％,24/597) of small for gestational age patients without single umbilical artery (x2=181.71,P＜0.01).Conclusions Single umbilical artery is likely to be complicated with congenital anomalies.After single umbilical artery was diagnosed by routine ultrasound,fetal ultrasonography,echocardiography and amniocentesis for karyotype analysis is suggested.Fetal growth restriction is an important indicator of fetal adverse outcomes.

Hyperthyroidism with periodic paralysis is common in clinic,the curative effect of peroral drugs on it is not satisfactory. In order to evaluate the treatment of 131 I for periodic paralysis in patients with hyperthyroidism, fouty two patients with hyperthyroidism complicated with periodic paralysis were put on p.o. 5.0 9.0 mCi 131 I Na treatment and the remission rate of periodic paralysis was observed.The total effective rate was over 95%. It can be concluded that 131 I could be used as a routine therapy for periodic paralysis in most patients with hyperthyroidism for its safety and efficiency.

Objective To observe the effect of ambroxol hydrochloride combined with phentolamine in adju-vant treatment of severe pneumonia in children.Methods According to the digital table,93 children with severe pneumonia were selected and randomly divided into A group,B group and C group with 31 cases in each group.Agroup with conventional measures for treatment of severe pneumonia in children for treatment,B group based on treatment scheme of A group,with ambroxol hydrochloride injection intravenous infusion in group B,group C foundationtreatment scheme,combined with phentolamine intravenous infusion.After 7d treatment in three groups were comparedthe clinical symptoms,signs and hospitalization days disappear,at the same time,the clinical effect between threegroups of children and to observe the adverse reaction.Results In C group,the cooling time for(1.98 ±1.25)d,heart failure free period of (1.71 ±0.92)d,cough disappeared time of (2.90 ±1.08)d,rales disappearing for(6.22 ±1.13)d,the time of hospitalization for (6.42 ±1.57)d,were significantly lower than those of A group(t =4.55,4.72,4.38,4.59,5.12,all P <0.05)and B group(t =3.98,3.76,4.12,4.08,4.14,all P <0.05),B groupof children with heart failure disappeared,antipyretic,cough disappeared,rales disappearing time was significantlylower than that in A group(t =3.97,4.01,3.88,3.56,all P <0.05);After treatment,in C group,the markedly effective rate was 41.94%,effective rate was 54.84%,total effective rate was 96.77%,which were significantly higherthan those in A group and B group(χ2 =10.33,5.165,all P <0.05);The three groups of children in the course oftreatment were not serious adverse reactions.Conclusion Ambroxol hydrochloride injection combined with phentolamine injection can quickly control of children with severe pneumonia disease,improve the clinical efficacy in children with severe pneumonia,and less adverse reactions,which is worthy of popularization and application.

AIM: To quantitatively detect the expression level of transforming growth factor-β1 (TGF-β1) and transforming growth factor-β2 (TGF-β2) genes in the retina of normal rat in order to determine the expression difference of TGF-β1 and TGF-β2 in retina.METHODS: The total RNA was isolated from which the first strand of cDNA was prepared. The mRNA levels of TGF-β1 and TGF-β2 were detected quantitatively by real time polymerase chain reaction (PCR).RESULTS: The mRNA levels of TGF-β1 and TGF-β2 were 0.0008±0.0003 and 0.0378±0.009, respectively. Expression of TGF-β2 was obviously higher than that of TGF-β1 in rat retina with statistical significance (t=12.37, P<0.001). The ratio of TGF-β2/TG-β1 was 55.00±26.61.CONCLUSION: QRT-PCR could specifically and accurately detect gene expression level in rat retina. In retina the TGF-β2 gene was expressed more abundantly than TGF-β1. It is suggested that TGF-β2 play an important role in retina diseases.

AIM: To quantitatively investigate transforming growth factor-β type Ⅰ receptor (TβRⅠ) and transforming growth factor-β type Ⅱ receptor (TβRⅡ) gene expressions in rat retina.METHODS: Sprague-Dawley rats were chosen in this research. Gene expression was detected quantitatively by reverse transcription polymerase chain reaction (RT-PCR) analysis. RESULTS: The expression level of TβRⅠ and TβRⅡ were 0.00034±0.00013 and 0.0001±0.00005, respectively. The expression level of TβRⅠ was obviously higher than that of TβRⅡ in the rat retina with statistical significance (P<0.01). The ratio of TβRⅠ/TβRⅡ was 3.9±1.7.CONCLUSION: Real time quantitative RT-PCR is an effective method to detect differential expression genes in retina. The change of TβRⅠ/TβRⅡ expression may play an important role in the pathogenesis of retinopathy, which could be further investigated in its significance in the development of proliferation retinopathy.