Objective: To investigate the awareness of relevant knowledge and influencing factors among high-risk populations for chronic obstructive pulmonary disease (COPD), so as to provide the reference for improving COPD awareness and disease prevention. Methods: The COPD high-risk populations were selected from community health service centers of Nanfeng, Jingang, Daxin and Fenghuang townships in Zhangjiagang City, Jiangsu Province from 2020 to 2022. Basic information, lifestyle, health status and awareness of relevant knowledge were collected through questionnaire surveys. Anxiety symptoms was assessed using the Generalized Anxiety Disorder Scale. Factors affecting the awareness of relevant knowledge among COPD high-risk populations were identified using a structural equation model. Results: A total of 2 078 COPD high-risk populations were surveyed, including 1 111 females (53.53%) and 967 males (46.47%), and had a mean age of (60.85±6.59) years. The total awareness score of relevant knowledge among high-risk populations for COPD was (6.33±2.59) points, with an awareness rate of 72.18%. Structural equation model analysis showed that age, annual household income, educational level, smoking, respiratory diseases, occupational exposure and anxiety symptoms had direct effects on the awareness of relevant knowledge, with direct effect values of -0.057 (95%CI: -0.099 to -0.014), 0.048 (95%CI: 0.005 to 0.090), 0.162 (95%CI: 0.117 to 0.204), 0.060 (95%CI: 0.018 to 0.096), 0.055 (95%CI: 0.021 to 0.088), 0.139 (95%CI: 0.107 to 0.170) and -0.172 (95%CI: -0.209 to -0.135), respectively; while age, gender, and occupational exposure also had indirect effects on the awareness of relevant knowledge, with indirect effect values of -0.069 (95%CI: -0.090 to -0.051), -0.084 (95%CI: -0.113 to -0.053), and 0.007 (95%CI: 0.003 to 0.014), respectively. Conclusion The awareness of relevant knowledge among COPD high-risk populations is associated with age, gender, annual household income, educational level, smoking, respiratory diseases, occupational exposure and anxiety symptoms.

Objective:To explore the effects of NOD2 gene on the proliferation and apoptosis of tongue squamous cell carcinoma Tca8113 cells.Methods:NOD2 expression vector(NOD2-pEZ-M29)and NOD2-shRNA vector were established,then were trans-fected into Tca8113 cells respectively.Expressions of HBD-2 and NOD2 in the cells was detected by RT-PCR and Western blot.Cell proliferation was examined by MTT assay and apoptosis by flow cytometry at 48h post transfection.Results:Compared with the control group,the expression of NOD2 and HBD-2 in NOD2-pEZ-M29 transfection group was significantly higher and markedly lower in NOD2-shRNA group.The proliferation rate of Tca8113 cells was markedly lower in NOD2-pEZ-M29 transfection group and signifi-cantly higher in NOD2-shRNA group while the apoptosis rate was significantly higher in NOD2-pEZ-M29 transfection group and sig-nificantly lower in NOD2-shRNA group.Conclusion:In Tca8113 cells NOD2 expression was positively correlated with HBD-2 ex-pression.NOD2 gene may promote the apoptosis,inhibit the proliferation of Tca8113 cells.

Objective : To investigate the prevalence of comorbid chronic obstructive pulmonary disease (COPD) and osteoporosis (OP) and its influencing factors among residents aged 40 years and older in Zhangjiagang City, Jiangsu Province, so as to provide insights into prevention of comorbid COPD and OP. Methods: Permanent residents aged 40 years and older were sampled using a multi-stage stratified cluster random sampling method in Zhangjiagang City in 2019, and their pulmonary functions and bone mineral density were measured. The prevalence of comorbid COPD with OP was estimated. The demographic features, smoking, diet, exercises and physical examinations were collected, and factors affecting the prevalence of comorbid COPD with OP were identified using a logistic regression model. Results: Totally 3 140 subjects were enrolled, including 1 315 men (41.88%) and 1 825 women (58.12%), and a mean age of (62.89±9.13) years. A total of 137 subjects were detected with comorbid COPD and OP, with prevalence of 4.36%. The patients with COPD alone included 19 cases with mild, 133 cases with moderate, 87 cases with severe and 26 cases with very severe COPD, and the patients with comorbid COPD and OP included 10 cases with mild, 56 cases with moderate, 56 cases with severe and 15 cases with very severe COPD. The grade of pulmonary functions was higher in patients with comorbid COPD and OP than in patients with COPD alone (Z=-12.304, P<0.001). Multivariable logistic regression analysis identified ages of 65 years and older (OR=2.703, 95%CI: 1.862-3.923), women (OR=2.897, 95%CI: 1.915-4.384) and physical labor (OR=1.540, 95%CI: 1.083-2.189) as factors affecting the development of comorbid COPD and OP. Conclusion The prevalence of comorbid COPD and OP was 4.36% among residents at ages of 40 years and older in Zhangjiagang City, and moderate and severe degree was the predominant grade of pulmonary functions. The elderly, women and physical labors are at a high risk of developing comorbid COPD and OP.