We present the results of determining Zn,Cu,Fe,Mn,Ca and Mg in the serum samples of 36 cases and hair samples of 42 cases.Comparing the patients of trophoblastic tumor with the local normal pregnant women in the same period,we found that in the patients' serum the amount of Zn decreased,and the amount of copper and the ratio of Cu/Zn increased evi- dently.They developed or went down along with the patients being worse or better.In the pa- tients' hair the amount of Zn decreased obviously and the ratio of Cu/Zn increased.The re- sults indicated that the determination of Zn in the seum and hair would be of great value in finding and diagnosing the disease early and the determination of Cu,Zn and the ratio of Cu/Zn with CP and HCG would provide a basis for the state of illness and it's prognosis.It was noted that the amount of manganese in the serum and hair of the patients was distinctly higher than those of the contrast.It provided an experimental basis for the prevention, diagnosis,treatment and prognosis of trophoblastic tumor.

Objective To investigate the effects of Kidney nourishing decoction in the treatment of reproduce patients in serum hormone .Methods 120 cases with reproduce patients from June 2014 to June 2016 in hangzhou traditional chinese medical hospital were selected,and divided into control group and experiment group, 60 cases in each group.The control group was given artificial cycle treatment, the experiment group was treated with Kidney nourishing decoction, two groups were treated for three months.Serum sex hormone and AMH content, clinical effect and pregnancy rate were compared before and after treatment.Results Compared with before treatment, levels of FSH, LH, E2 and FSH/LH in two groups decreased, AMH content increased after treatment,the thickness of endometrium and ovarian antral follicle number increased,symptom scores decreased after treatment (P <0.05);Compared with the control group, levels of FSH, LH, E2 and FSH/LH in the experiment group were lower, AMH content were higher, the thickness of endometrium and ovarian antral follicle number in the experiment group were higher,and the symptom scores were lower (P<0.05).The total effective rate of the study group was 90.00% higher than the total effective rate of the control group 73.33% (P<0.05).There was no significant difference in adverse reaction rate between the two groups, the pregnancy rate in the experiment group 30.00% was higher than the control group 11.67% ( P <0.05 ) .Conclusion Kidney nourishing decoction can reduce the sex hormone and increase the content of AMH , and increase the pregnancy rate in the treatment of reproduce patients.

Objective To investigate the effect of Interleukin(IL)-18,IL-12 and tumor necrosis factor-α(TNF-α)in hepatic injury in intrahepatic cholestasis of pregnancy(ICP).Methods Sixty-two cases of ICP patients(ICP group),30 cases of normal pregnant women(control group)and 30 cases of hepatitis B(HBV) women (hepatitis group) were recruited. Serum IL-18, IL-12 and TNF-α were examined by ELISA. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were examined by automatic biochemical analysis instrument. Results ( 1 ) In hepatitis group, serum concentrations of IL-18,IL-12 and TNF-α were (256±51 ) ng/L, ( 122±96) ng/L and (207±3) ng/L; serum levels of ALT and AST were(363±174) U/L and (359 ±237) U/L, respectively. In ICP group, serum concentrations of IL18, IL-12 and TNF-α were (72±32) ng/L, (42 ±28) ng/L and (48±14) ng/L; serum levels of ALT and AST were (201 ±128) U/L and ( 132±87) U/L, respectively. While in control group, serum concentrations of IL-18, IL-12 and TNF-α were (43 ± 13) ng/L, ( 10±3) ng/L and (33±9) ng/L; serum levels of ALT and AST were (13 ~ 4) U/L and (15 ± 3) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in hepatitis group were significantly higher than those in ICP group and control group ( P ＜0. 05 ).Serum IL-18, IL-12, TNF-α, ALT and AST levels in ICP group were significantly higher than those in control group(P ＜ 0. 05 ). (2) In severe ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (81 ±32) ng/L, (50 ±25) ng/L and(50 ± 14) ng/L; serum levels of ALT and AST were (269 ± 111 ) U/L and (181±73) U/L In mild ICP subgroup, serum concentrations of IL-18, IL-12 and TNF-α were (48 ±18 ) ng/L, (17 ± 4 ) ng/L and (40 ± 10 ) ng/L; serum levels of ALT and AST were (87±46) U/L and (50 ±21 ) U/L, respectively. Serum IL-18, IL-12, TNF-α, ALT and AST levels in severe ICP subgroup were significantly higher than those in mild ICP subgroup and control group (P ＜ 0. 05). And serum ALT and AST levels in mild ICP subgroup were significantly higher than those in control group(P ＜0. 05). (3) There were 16 cases with preterm birth (50%, 16/32 ) and 10 cases with meconium-stained amniotic fluid( 31%, 10/32 ) in severe ICP subgroup, significantly higher than those in mild ICP subgroup ( P＜ 0. 05 ), which contained 2 preterm births ( 7%, 2/30) and 1 meconium-stained amniotic fluid (3%, 1/30). While in control group, the numbers were 1(3%, 1/30)and 1(3%, 1/30),respectively. As for the cases of neonates whose 1 minute Apgar score were not more than 7, there were 2 cases, 1 case and 1 case in severe ICP subgroup, mild ICP subgroup and control group, respectively,which showed no significant difference(P＞ 0. 05). Conclusion Serum IL-18, IL-12 and TNF-α may be involved in the process of hepatic injury of ICP.

Objective To investigate the efficacy and safety of anterior pelvic floor reconstruction with transobturator tension-free vaginal mesh in the treatment of moderate to severe or recurrent anterior vaginal wall prolapse. MethodsAnterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh were performed on 36 women with stage 3 to stage 4 or recurrent anterior vaginal wall prolapse.Those with stress urinary incontinence(SUI) or potential SUI were also treated with transobturator inside-out tension-free vaginal tape(TOT).The surgical efficacy was determined by comparison of POP-Q classification system pre-and post-operation.Complications of the procedure were statistically analyzed. Results The mean time of operation for anterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh was 52 min,mean intraoperative blood loss was 55 mL,and mean hospital stay was 3.5 d.No operative complications occurred.All the anterior vaginal wall prolapse was corrected.No recurrence was observed after the follow up for 2 to 24 months,with the effective rate of 100%.The grade of urinary incontinence was improved remarkably,with the effective rate of 100%.The erosion after the procedure with vaginal polypropylene mesh happened in 2 cases,with the incidence of 5.56%. Conclusion Anterior pelvic floor reconstruction with transobturator tension-free vaginal polypropylene mesh is a minimally-invasive procedure for the treatment of moderate to severe or recurrent anterior vaginal wall prolapse.TOT should also be performed for those with SUI or potential SUI.Though erosion of mesh may take place post-operation,this procedure is safe and has a favourable short-term effect.The long-term effect leaves for further investigations.

ObjectiveTo evaluate two "up and down stair" methods used by hemiplegic patients.Methods40 cases with hemiplegia were randomly divided into two groups with 20 cases in each group. Patients in the group A went upstairs with health leg and downstairs with affected leg, while, patients in the group B upstairs with affected leg, downstairs with health leg, and then up and down stairs with health and affected leg alternated. Effects of two training menthods were compared.ResultsEach of two training methods had its advantage and disadvantage, but motor function and mobile ability of patients in the group B were better than patients in the group A (P<0.05).ConclusionHemiplegic patients Should choose different training method of up and down stairs according to his status, and not always choose the method of going upstairs with health leg and downstairs with affected leg.

Ear Protective Devices ; Follow-Up Studies ; Hearing Loss, Noise-Induced ; prevention & control ; Humans ; Male ; Mining ; Noise, Occupational ; prevention & control ; Petroleum ; Retrospective Studies

Using Isopropyl amine (0.003 mol/L), p-methyl-phenol (0.03 mol/L) and methanol (1 mol/L) for simulating amino groups, phenol groups and hydroxyl groups in silk fibroin respectively, the reaction rate of different nucleophilic groups in silk with monochlorotriazine reactive dye was studied at various temperature and pH by high performance liquid chromatography. It was found that the overall reaction rate of dyes′ phenolysis was by far higher than that of ammonolysis and alcoholysis on the conditions of 70-95 ℃ and pH=8-10. The phenolysis overall reaction rates of C.I. reactive red 24 and C.I. reactive orange 2 were 8.5 and 12.5 times of their ammonolysis′, and 23 and 50 times of their alcoholysis′ in the term of pH 9 and 95 ℃. The phenolysis efficiencies of those two dyes were 47.4 and 96.3, the ammonolysis efficiencies were 4.6 and 6.9, and the isopropyl amine, p-methyl-phenol and methanol to simulate the silk fibroin, the reaction selectivity between the monochlorotriazine reactive dyes and nucleophilic groups in silk fibroin was investigated. It was deduced that monochlorotriazine reactive dyes are suitable for dyeing silk on the conditions of about 85 ℃ and pH=8-9, and under this condition, phenol groups are the major groups in silk dyeing with monochlorotriazine reactive dyes, amino groups are the minor groups, and the contributions of hydroxyl groups can be neglected.

ATP-Binding Cassette, Sub-Family B, Member 1 ; biosynthesis ; Animals ; Brain ; metabolism ; Disease Models, Animal ; Glycoproteins ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Status Epilepticus ; metabolism

ATP Binding Cassette Transporter, Sub-Family B ; metabolism ; Animals ; Anticonvulsants ; pharmacology ; Brain ; drug effects ; metabolism ; Rats

Objective To explore the change of degrade of clonazepam in serum with single and repeated - dose administration in children with seizure, and find a reasonable method for using the clonazepam. Methods Children with seizures were divided into single - dose paradigam, repeated - dose paradigam, and decreased - dose paradigam. The concentration of CZP in serum was determined by high performance liquid chromatography( HPLC). Results The serum concentrations of clanazepain in single - dose paradigam were (101.9?12.1),(76.9 ? 5.8),(50.7?2.9),(30.9?5.4),(21.5?6.8)?g/L,the time point that the blood samples collected were 15,30,60,120 and 480 min. The serum concentrations in repeated - dose paradigam were (97. 2 ? 6. 1),(130.4? 13. 4), (99. 4 ? 9.8),(79.6?2.4)?g/L,in decreased-dose paradigam were( 101.1 ?13.1),(123.1 ?6. 6), (99.4 ?9. 8), (79. 3 ? 2. 2)?g/L,in these two groups,the time point were 15,45,60 and 120 min. Conclusion Repeated administration of CZP with decreased dose may increase its effectiveness in treatment without substantially increasing toxicity.