【Purpose】 To investigate effects of physiotherapy on delayed onset muscle soreness (DOMS), DOMS was induced in the elbow flexors of the non-dominant arm. We selected transcutaneous electrical nerve stimulation (TENS) to examine effects of physiotherapy to the recovery process.【Methods】 Fifteen human volunteers were recruited. DOMS was induced by eccentric exercise. Subjects were assigned randomly to one of three groups (Control, 110 Hz TENS, 4 Hz TENS). TENS was applied for 20 min over the biceps brachii on 1 day only after exercise. Measurements of Mechanical Pain Threshold (MPT) and Visual Analogue Scale (VAS) were performed as evaluation of pain. Measurements were taken before and after exercise.【Results】 There was no significant improvement in MPT and VAS among all three groups.【Conclusion】 It was suggested application of TENS on DOMS at the parameters used here had no beneficial effect.