The +2 ions in the extenal fluids of the cells touching the outer surface of the nerve membranes are indespensible to excitation; if a +1 ion is added, excitation becomes easy. In the inner fluids touching the inner walls of the membrane with the existence of +1 ions only there is no problem, but if a +2 ion happens to come to exist within these inner fluids generally, this results in a decrease in the active electrical potential.
Very little is known about the quantitative tendencies of trace metal elements (or ions) within the local domain of the human tissue however the verification of the amounts of VIa (Cr, Mo), VIIa (Mn), VIII (Fe, Co, Ni, Pt), Ib (Cu, Ag, Au) transition metal elements and IIb (Zn, Cd) among the heavy metal elements existing in the human body is a matter of great physiological and pathological importance.
We will report information concerning metals among heavy metals and the transition metals in which characteristic X-rays appeared notably in this experiment.
The results of this experiment indicate the following: (1) We can induce the equation of diffusion and distribution concerning to the ions of metal element introduced into the skin and the muscle. (2) The amount of ionization of metal elements introduced into the tissues is not abided by Farady's law of electrolysis. (3) In proportion as time of stimulation increases the amount of K-ions in the local domain punctured by Au-needle. (4) As the effect of metal element punctured and introduced into the tissues, the ions of the transition metal elements that were already existing in the tissues are acting in conjunction with the introduced element. (5) The cause of variation on the trace element is not analyzed in this experiment.