1.Microstructure Analysis of Immobilized Osteopenia Using Microcomputed Tomography：An In Vivo Study
Hironobu Koseki ; Makoto Osaki ; Hidehiko Horiuchi ; Yuichiro Honda ; Ryo Sasabe ; Junya Sakamoto ; Takashi Higuchi ; Sinya Sunagawa ; Minoru Okita
The Japanese Journal of Rehabilitation Medicine 2017;54(9):718-723
Objective：To assess the effects of immobilization on the three-dimensional microstructure of cortical and cancellous bone.
Methods：Eight-week-old specific-pathogen-free Wistar rats were divided into two groups：the control (n＝12) and immobilized group (n＝12). The hind limbs of the rats in the immobilized group were fixed using orthopedic casts for 4 weeks. The cortical bone at the mid-shaft and the cancellous bone at the distal metaphysis of the femur were analyzed using micro-computed tomography.
Results：The values of total cross-sectional area, cortical bone area, and cortical thickness in the immobilized group were significantly lower than those in the control group. Meanwhile, the relative bone volume (bone volume/total volume) and mean trabecular thickness in the immobilized group decreased and the structure model index significantly increased compared with the values in the control group (P＜0.05).
Conclusion：Our rat model can evaluate the effects of load-permitting immobilization. The results of this study indicate that structural changes in immobilized osteopenia-affected bone arise mainly from thinning of the cortex and reduction of cancellous bone volume caused by a reduction of trabecular width.
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