Objective To establish cell suspension culture system of Gentiana macrophylla.Methods Good cell line was selected by the methods of small cell aggregate and the effects of different media,inoculation age of cells,initial pH value,culture temperature,and rotation speed on cell growth and gentiopicroside accumulation were investigated.Results The eligible cell suspension culture conditions were:MS media as the basic medium,cells with inoculation age of 15 d,initial pH 7,and rotation speed of 110 r/min at 25 ℃,culturing in Erlenmeyer flasks.Conclusion Optimal cell suspension culture system of G.macrophylla has been established by shake flasks.The different effects of media on cell growth and gentiopicroside accumulation indicate that some inorganic ions have significant effects on regulation.

Objective To investigate the effects of carbohydrate and its concentration,nitrogen source,and metal ions on suspension cell growth of Gentiana macrophylla and gentiopicroside accumulation. Methods The cell dry weight and gentiopicroside content were measured under factors of different carbohydrate,NH4+/NO3-,Ca2+,and Zn2+ at various concentration. Results The optimum culture conditions are as follows:3% sucrose,NH4+/NO3-of 20/40,3 mmol/L Ca2+,0.007 49 mmol/L Zn2+. Conclusion Different carbohydrates and their concentration have significant effects on cell growth and gentiopicroside accumulation while high concentration of carbohydrates does not have such effect. NO3-is better than NH4+ for the cell growth. Ca2+ Remarkably influences both cell growth and gentiopicroside accumulation while Zn2+ contributes only to gentiopicroside content.

Objective To summarize the experience of nursing cancer patients with Wernicke encephalopathy (WE) after gastrointestinal surgery. Method The clinical data of 6 patients with WE after gastrointestinal surgery in our hospital from January 2005 to June 2015 were retrospectively analyzed to summarize the nursing strategies. Results All the 6 patients showed mental changes of different types, 5 patients developed with ocular manifestations and one patient with ataxia. Only one patient showed the classic triad of the disease at clinical presentation. All the 6 patients had characteristic manifestations on cranial MRI and received thiamine (vitamin B1) at diagnosis. Four patients were completely recovered and 2 were partially recovered. Conclusion Timely sufficient thiamine supplementation during the perioperative period is the key to prevent and treat WE in the patients with gastrointestinal cancer. Careful observation of the patients′ mental state and the effective nursing for the special classic triad of the disease are critical for a better prognosis.

BACKGROUND: Trigeminal ganglion percutaneous puncture is one of the methods for the therapy of primary trigeminal neuralgia. The difficulties in percutaneous puncture technique and operation can lead accidental injury and some serious complications. The application of DZY-C trigeminal nerve stereoguide in the treatment of trigeminal neuralgia has the features of high accuracy in puncture and low complications, which can reduce the chances of accidental injury to surrounding vessels and nerves.OBJECTIVE: To evaluate the therapeutic effects of DZY-C trigeminal nerve stereoguide in the treatment of trigeminal neuralgia.DESIGN: A before-and-after experimental study based on patients.SETTING: Department of cerebral surgery of a municipal hospital of tradi tional Chinese medicine and a municipal hospital.PARTICIPANTS: Ninety patients including 39 male and 51 female patients with primary trigeminal neuralgia aged between 21 and 90 years old were selected from outpatient or inpatient department of Foshan Hospital of Traditional Chinese Medicine between 2001 and 2003.INTERVENTIONS: Glycerine was injected into trigerminal ganglion of each patient through percutaneous puncture under the guidance of DZY-C trigeminal nerve stereoguide. Therapeutic effects were evaluated by visual analogue scale(VAS) before and 30 minutes after the treatment.MAIN OUTCOME MEASURES: Results of therapeutic effects.RESULTS: Ninety patients with primary trigeminal neuralgia received trigeminal ganglion puncture with the application of DZY-C trigeminal nerve stereoguide. All punctures succeeded with one shot. After the injection of glycerine, the original trigeminal neuralgia disappeared in all 90 cases with the good and excellent rate of 100%.CONCLUSION: Trigeminal nerve stereoguide(DZY-C model) has the features of reasonable structural design, easy surgical operation, agile adjustment, less tissue injury, and safe manipulation, which can significantly eliminate trigeminal neuralgia.

Objective To evaluate the role of nuclear factor kappa B (NF-κB) in sevofluraneinduced release of inflammatory factors in mouse microglias.Methods Microglial cells obtained from newborn C57BL/6 mice (aged 2-3 days) were seeded in 24-well plates (density 1 × 105 cells/ml, 1 ml/well) , a total of 80 wells.The cells were randomly divided into 4 groups using a random number table (n =20 each) : control group (group C);sevoflurane group (group S);NF-κB selective inhibitor pyrrolidine dithiocarbamate (PDTC) group (group P);PDTC + sevoflurane group (group P +S).In S and P+S groups, 4.1％ sevoflurane was inhaled for 6 h.In P+S group, 10 μmol/L PDTC was added at 1 h before sevoflurane inhalation.At 6 h of incubation with sevoflurane, NF-κB activity and expression and levels of interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were determined.Results Compared with group C, the NF-κB activity and levels of IL-6 and TNF-α were significantly increased in group S, and no significant change in the above parameters was found in the other two groups.Compared with group S, the NF-κB activity and levels of IL-6 and TNF-α were significantly decreased in group P+S.There was no significant difference in NF-κB expression between the four groups.Conclusion NF-κB mediates sevoflurane-induced release of inflammatory factors in mouse microglias.

Objective To observe the effects of electro-acupuncture on Jiaji acupoints combine with treadmill training on tibia bone mass of rabbits with sciatic nerve injury,so as to provide the theoretical support for better project to maintain normal metabolism of bone after peripheral nerve injury.Methods A total of 24 New Zealand rabbits were randomly divided into four groups (n =6),namely a model group,a sham group,a treadmill group and an electro-acupuncture + treadmill group according to a random number table.The sciatic nerve of rabbits in the model,treadmill and electro-acupuncture + treadmill group was clamped using hemostatic forceps to establish the model of sciatic nerve injury,while that of the sham group was not clamped,just cut soft tissue around the sciatic nerve.Three days after modeling,the rabbits in treadmill group and the electro-acupuncture + treadmill group were given treadmill training and electro-acupuncture combine with treadmill training respectively,lasting 4 months,whereas those in the sham and the model groups were not given any intervention.Right after modeling,the behavior of rabbits in four groups was observed.Four weeks after treatment or feeding,their bone mineral density and bone strength of tibia were measured.Results Four weeks after treatment,there was significant reduction in bone density and bone strength of tibia in the model,the treadmill and the electro-acupuncture + treadmill groups compared with those in the sham group(P ＜ 0.05) ; the bone mineral density and bone strength of tibia in the electro-acupuncture + treadmill group were 0.17 ± 0.01 g/cm2 and 161.92 ± 43.27 N respectively,significantly higher than those in both the model and the treadmill groups (P ＜ 0.05).Conclusion Electro-acupuncture combined with treadmill training can improve tibia bone mineral density and bone strength after sciatic nerve injury and facilitate the normal metabolism of the bone and to maintain its normal function.

Objective To study the analepsia effect of high and low dose sevoflurane combined with propofol anesthesia in partial hepatectomy.Methods 64 patients with partial hepatectomy were selected as the subjects.They were randomly divided into low concentration group and high concentration group,32 patients in each group.The two groups were treated with sevoflurane combined with propofol anesthesia,low concentration group was given 0.5MAC sevoflurane,high concentration group was given 1.0MAC sevoflurane.The clinical results were compared between the two groups.Results The time of propofol use,postoperative recovery time,extubation time and improved Aldrete score of 9 min in the high concentration group were(765.83±342.86)mg,(21.39±8.23)min,(41.81±8.37)min and(30.24±9.63)min,respectively,which were significantly lower than those in the low concentration group [(1 143.64±374.64)mg,(25.46±7.98)min,(46.68±7.73)min and(35.94±8.89)min,respectively,t=4.208,0.008,2.418,2.460,all P<0.05].In addition,the OAA/S scores at T0,T1of the high concentration group were(3.83±0.64)points and(4.28±0.85)points,respectively,which were significantly higher than those of the control group[(3.36±0.76)points and(3.79±0.87)points],the differences between the two groups were statistically significant(t=2.676,2.279,all P<0.05).Conclusion The combination of sevoflurane and propofol in patients with partial hepatectomy can reduce the dosage of propofol and shorten the recovery time of postoperative spontaneous breathing in patients,and contribute to improve the quality of patients'' recovery.

Objective To observe the clinical efficacy of diltiazem in patients with coronary disease following percutaneous coronary intervention(PCI).Methods From Jan 2014 to Jan 2016,116 cases of coronary disease were selected as the research subjects,they were randomly divided into the observation group(62cases)and the control group(54 cases).The control group was given conventional therapy,while the observation group was given diltiazem therapy on the basis of control group.The levels of BP,HR,NT -proBNP,CRP and angina frequency in the two groups were compared before and 24h,48h after PCI.The incidence of major adverse cardiovascular events(MACEs) was assessed at the sixth month after PCI.Results After surgery,the CRP levels of the observation group and control group were (40.1 ±7.4)mg/L and (41.7 ±8.8)mg/L,the NT -proBNP levels of the two groups were (501.8 ± 56.5)ng/L and (445.7 ±50.6)ng/L,which were significantly higher than preoperation(t =5.684,P <0.05).The blood pressure,heart rate,CRP,NT -proBNP of the observation group since 24 hours after PCI were (73.1 ± 6.2)mmHg,(121.7 ±15.8)mmHg,(68.1 ±7.9)times/min,(24.8 ±3.7)mg/L,(201.7 ±20.6)ng/L,which were significantly lower than those of control group [(85.6 ±9.3 )mmHg,(134.3 ±17.4)mmHg,(77.5 ± 9.2)times/min,(36.5 ±7.9)mg/L,(481.5 ±48.2)ng/L].The effect was kept over 48 hours(F =7.281,8.097, 6.945,7.682,6.517,all P <0.05).Angina pectoris after treatment in the observation group and control group were (1.5 ±0.9)times/month,(2.9 ±0.7)times/month,which were significantly lower than before treatment(t =7.584,P <0.05).The incidence rate of total MACE in the observation group was 4.84%,which was significantly lower than that in the control group(χ2 =6.942,P <0.05).Conclusion Diltiazem after PCI can decrease the adverse reaction, and help to improve the prognosis of patients.

Objective To observe the efficacy of de novo combination therapy lamivudine plus adefovir , lamivudine monotherapy and entecavir monotherapy in HBeAg-positive CHB patients with genotype B/C. Methods A total of 182 treatment-naive CHB patients in line with treatment standards of Chinese CHB prevention and treatment guidelines were randomly assigned to three groups and treated with lamivudine plus adefovir or lamivudine monotherapy or entecavir monotherapy for 48 weeks. Results Patients in three groups presented no difference in baseline levels. After treatment by three therapies , the group of lamivudine plus adefovir showed a higher biochemical response rates (12 week P < 0.01, 24 week P < 0.01, 48 week P < 0.01), HBeAg-serological rates(12 week P < 0.01, 24 week P < 0.05, 48 week P < 0.05) and completely virological response rates (12 week P < 0.05, 24 week P < 0.05, 48 week P < 0.05) than lamivudine group. In terms of biochemical response rates , the group of lamivudine plus adefovir had certain advantages when compared with entecavir group. Conclusion De novo combination therapy lamivudine plus adefovir is a good antiviral strategy for chronic hepatitis B patients with B/C genotype viral infection in China.

From August 2011 to March 2012,5 241 type 2 diabetic patients with body mass index ≥ 24kg/m2 were enrolled from 60 hospitals in Guangdong Province.According to estimated glomerular filtration rate (eGFR),a total of 2 631 subjects with norml urine microalbumin level (＜30 ng/L) were divided into normal eGRF group and decreased eGRF group.Binary logistic regression was used to analyze the associations between eGFR and its related risk factors.The results showed that age,blood uric acid,blood urea nitrogen,history of hypertension and coronary heart disease,family history of diabetes,and hyperuricemia were positively related to lowering of eGFR (P＜ 0.05 or P＜0.01).HbA1C＜7％,regular glucose monitoring,and regular physical activity were negatively related to eGFR decrease (all P＜ 0.01).These results suggest that urine microalbumin and eGFR should be applied to overweight or obese patients with type 2 diabetes in order to screen diabetic nephropathy.Furthermore,intensive control of blood glucose,uric acid,and blood pressure is beneficial to lowering the risk of diabetic nephropathy.