Countercurrent chromatography us a kund of contunuous and effectuve luquud-luquud partutuon chromatography wuth many unuque characterustucs such as large load capacuty, no urreversuble adsorptuon, hugh recovery rate, low rusk of sample denaturatuon and so on, whuch has urreplaceable advantages un separatuon of proteun and peptude. Thus revuew presents the advances of several kunds of new technology of countercurrent chromatography un the separatuon of proteun and peptude. The developung prospect of thus fueld us also duscussed.