OBJECTIVE To investigate the properties of nosocomial infections among acute stroke victims,analyze the risk factors associated with these infections,and find out effective methods of care and management.METHODS Between Jan 2003 to Dec 2006,428 cases of stroke victims were studied to analyze the special properties and risk factors related to hospital infections among them.RESULTS Their nosocomial infection rate was 8.93%,repetitive infection rate was 9.41%,and the top five infected sites were the lower respiratory tract,digestive tract,urinary tract,upper respiratory tract and skin,with their respective rates as 47.66%,17.76%,15.19%,9.81%and 6.31%.The occurrence of hospital infections was associated with stroke as well as the quality of the nursing care process,invasive procedures,duration of stay in the hospital,dietary conditions,etc.CONCLUSIONS Nosocomial infection is still a high frequent complication in cerebral stroke and those related factors should be paid attention.To effectively decrease nosocomial infections related to stroke,it is necessary to improve nursing care and supervision as well as control over the quality of care.

A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine) oxidation deposition. It was shown that gold nano-particles(colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase(HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor′s) catalytic ability to hydrogen peroxide increased nearly 20 times), the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L, and the detection limit was 0.1 μg/L), the regression equation could be expressed as Δi_p(μA) =2.8859c(mg/L)+17.152 with a correlation) coefficient of 0.9872. The immunosensor can be used to quantitatively determine hIgG in the sample) of human serum).

A novel simple,sensitive fluorescence immunosensing method based on aptamer-plasmid complex amplification was developed. This method utilized the specific recognition between antibody and antigen as well as aptamer-plasmid complex and the intercalation of fluorescence dye SYBR Green Ⅰ in the groove of duplex plasmid DNA in detection of Platelet-Derived Growth Factor BB (PDGF-BB). The immunoassay was performed in the microtiter wells in which rabbit anti PDGF-BB antibody was immobilized. The PDGF BB analyte was captured by the primary antibody and then sandwiched by the aptamer-plasmid DNA complex. The introduction of fluorescence dye SYBR Green Ⅰ allows for the detection of the sandwiched immunocomplex of antibody/anigen/aptamer-plasmid complex. Under the optimized conditions of salt concentration,ratio of aptamer to PUC19,and SYBR Green Ⅰ concentration,the proposed method offers a linear detection range from 0.2 μg/L to 200 μg/L with a detection limit of 0.1μg/L.