Objective To investigate the hand washing state and its influencing factors in patients undergoing infusion in outpatient clinic in order to adopt measures to enhance the patients' consciousness of hand hygiene. Methods 300 adult patients undergoing infusion in outpatient clinic were investigated.The bacteria condition before and after washing hands was observed. The condition and washing apparatus in the infusion room were also evaluated. Results The bacteria amount in the hands of patients undergoing infusion was 20 to 36 times higher than the standard level. The washing apparatus was simple and did not reach the standard requirement. Conclusion We should strengthen the propaganda of hand hygiene knowledge to patients undergoing infusion and improve their recognition of the importance of hand washing. The hospital should improve the washing facilities to reduce hospital infeciton.

Objective To explore the effect of relaxation therapy on rehabilitation patients with functional dyspepsia.Methods 112 cases with functional dyspepsia were randomly divided into test group(n=56) and control group(n=56).Relaxation trains was adopted in test group,usual nursing care was adopted to the patients in both groups.The therapeutic effects and relapse rate were observed in two groups. Results There were no significant differences of clinical curative efficacy in two groups first-visit and less than six-month pathogenesis patients,but compared with the control group,the cure efficacy of recurrent-attack patients and over six-month-pathogenesis patients were significantly lower in test group,and the relapse rate in control group is higher than that in test group. Conclusion The relaxation therapy can promote the rehabilitation in functional dyspepsia patients of recurrent attack and over-six-month pathogenesis,and decrease the relapse rate.

Objective To observe the effects of electroacupuncture on the motor function and mitochondrial dynamics of skeletal muscle of SAMP8 mice;To explore the mechanism of electroacupuncture in improving the motor dysfunction of Alzheimer disease(AD)from the perspective of mitochondrial dynamics.Methods Totally 18 SAMP8 mice were divided into model group and electroacupuncture group,with 9 mice in each group,and the SAMR1 mice with the same age were set as control group."Baihui","Dazhui"and"Shenshu"were selected in the electroacupuncture group,and electroacupuncture was performed daily for 20 min,8 d as a course of treatment.Each course of treatment was separated by 2 d,for a total of 3 courses of treatment.The model group and the control group were not intervened.The motor function of mice was tested by grip strength test,suspension test,hind limb extension test and Morris water maze experiment.The morphology and structure of gastrocnemius were observed by HE staining,ATP content in gastrocnemius was determined by colorimetry,the mRNA expression of optic atrophy 1(OPA1),mitofusin 2(MFN2)and dynamin-related protein 1(DRP1)in gastrocnemius were detected by real-time quantitative PCR,the expressions of OPA1,MFN2 and DRP1 in gastrocnemius were detected by Western blot.Results Compared with the control group,the grip strength,the score in suspension test,and the average speed and maximum speed of Morris water maze experiment of mice in model group significantly decreased(P<0.01);the arrangement of fibers in the gastrocnemius muscle tissue was disordered,the gaps become wider,and the distribution of nuclei was uneven;the ATP content in the gastrocnemius muscle tissue was significantly decreased(P<0.01),the mRNA and protein expressions of OPA1 and MFN2 were significantly decreased(P<0.01),and the expression of DRP1 mRNA and protein significantly increased(P<0.01).Compared with the model group,the grip strength,the score in suspension test,and the average speed and maximum speed of Morris water maze experiment in electroacupuncture group significantly increased(P<0.01);the arrangement of gastrocnemius muscle tissue was relatively neat,the gaps become narrower,and the distribution of nuclei was more uniform;the ATP content in gastrocnemius muscle tissue significantly increased(P<0.01),while the mRNA and protein expressions of OPA1 and MFN2 significantly increased(P<0.05,P<0.01),the expression of DRP1 mRNA and protein significantly decreased(P<0.01).Conclusion Electroacupuncture can improve the skeletal muscle morphological structure and motor dysfunction of SAMP8 mice,and the mechanism may be related to the correction of skeletal muscle mitochondrial dynamic imbalance and the increase of skeletal muscle ATP content.