Objective To investgate the efficiency of autologous fat grafting for breast augmentation by improving low negative pressure mechanical liposuction.Methods A total of 37 cases of breast augmentation by autologous fat grafting were reviewed;group A included 19 cases of improved low pressure mechanical liposuction,and group B had 18 cases of traditional syringe liposuction.Respectively,the operating time of harvesting 800 ml fat granules (standing 30 mins) were recorded,and operative blood loss by testing the red blood cell count of lower mixed liquid was calculated,and then growth increment of nipple chest circumference of 6 months after operation were measured.The valid follow-up satisfaction scores were counted.Results The operating time of harvesting 800 ml fat granules were (53.32±4.73) mins for group A and (80.78±7.86) mins for group B,respectively;operative blood loss was (81.58±10.80) ml for group A and (72.94±13.45) ml for group B;All P＜0.05.The valid follow-up satisfaction scores were 78.9％ (15/19) for group A and 77.7％ (14/19) for group B.Growth increment of nipple chest circumference of 6 months after operation between groups were no statistical significance (P＞0.05);but the remarkable difference within both groups in internal comparison existed between 6 months and 1 month after operation (P＜0.05),also between 3 months and 1 month after operation (P＜0.05).Conclusions Improved low pressure mechanical liposuction in breast augmentation by autologous fat grafting is able to enhance the surgical efficiency evidently and shorten the operating time significantly.

Objective To explore the application of diffusion tensor imaging in the assessment of ischemic muscle in hind-limb ischemia model of rabbit. Methods Excision of femoral artery in unilateral hind limb was done in 14 New Zealand white rabbits and ischemic model were established in 12 rabbits. Three (12 rabbits),10(10 rabbits) ,28(7 rabbits),56(5 rabbits) days after the model establishment, DTI scan was performed on bilateral hind limbs in each of the models, respectively and, λ_1,λ_2,λ_3, ADC and FA values were measured. Histological analysis was also performed at these time points. Pared t test was used to compare the differences of these indexes in bilateral hind limbs. Results Following femoral artery excision, a rapid ascending of ADC,λ_2,λ_3 values with sharply reduced FA value was observed in ligated hind-limb, which reached maximal on 3 days post-excision (ADC_(ligated)= 1.72±0.16, ADC_(unligated)= 1.53±0.16, t = 6.48, P < 0.01 ; λ_(2 ligated)= 1.70±0.15, λ_(2 unligated)= 1.51±0.06, t=10.87, P < 0.01 ; λ_(3 ligated_ =1.17±0.12, λ_(3 unligated)= 0.88±0.12, t=6.67, P < 0.01 ; FA_(ligated)= 0.24±0.04, FA_(unligated) =0.39±0.03, t = -10.61 ,P <0.01) and histologic analysis revealed the severest muscle damag at that time. Ischemic muscle recovered very slowly during the first 10 day post-excision accompanied with reduction of ADC , λ_2, λ_3 values, however there was also difference of ADC,λ_2, λ_3 values between ligated and nonligated limbs except λ_1 ( ADC_(ligated) = 1.65±0. 16, ADC_(unligated)= 1.50±0.12, t =6.42, P <0.01 ; λ_(2 ligated) = 1.62±0.32, λ_(2 unligated) =1.48±0.31, t=5.09, P < 0.01) ; λ_(3 ligated)= 1.11±0.13, λ_(3 unligated)= 0.85±0.09, t=6.26, P <0. 01;λ_(1 ligated)=2.20±0.21, λ_(1 unligated) =2.18+0.20, t=0.87, P=0.40). After 28 days, ADC and λ_3 returned to normal (ADC_(ligated)= 1.51±0. 16, ADC_(unligated)= 1.55±0.14, t=-1.35, P=0.23 ; λ_(3 ligated) =0.95±0. 10, λ_(3 unligated)= 0.92±0.06, t=1.70, P=0.14), but λ_2 and FA of ligated limb were still different from those of nonligated limb (λ_(2 ligated)= 1.45±0.23, λ_(2 unligated)= 1.52±0. 95, t=-3.56, P=0.012; FA_(ligated)=0.35±0.02, FA_(unligated)=0.40±0.03, t=-3.83, P<0.01). After 56 days, all parameters retuned to normal(ADC_(ligated) =1.57±0.18, ADC_(unligated)=1.58±0.23, t=-0.71, P=0.52; λ_(1 ligated) =2.18±0.18, λ_(1 unligated)=2.24±0.14, t=-0.22, P=0.10; λ_(2 ligated)=1.64±0.13, λ_(2 unligated)=1.59±0.15, t=0.89, P=0.42; λ_(3 ligated)=0.89±0.1,λ_(3 unligated)=0.91±0.07, t=- 1.64,P= 0.18; FA_(ligated)= 0.39±0. 03, FA_(unligated)= 0.41±0.02, t=-0.83, P=0.47). Conclusion DTI is a quantitative and relatively accurate technique to assess time-dependent changes of ischemic muscle in hindlimb ischemia model of rabbit.

Objective To explore the relation between the enlargement of orbital volume and the degree of enophthalmos, and that between the enlarged volume of floor together with that of medial wall and the degree of enophthalmos. Methods A total of 17 patients of unilarteral orbital fracture were scanned by MSCT (slice width 0.625mm), who suffered late enophthalmos as a results of combination fracture of the medial wall and floor. The clinical data were collected, and input into a software named MIMICS in order to measure orbital volume, the degree of enophthalmos, the enlarged volume of medial wall, and that of orbital floor. Simple linear correlation and regression were carried out between the enlargement of orbital volume and the degree of enophthalmos. Mutiple linear correlation and regression were carried out between the enlarging volume of floor together with that of medial wall and the degree of enophthalmos. Results The equation of the enlargement of orbital volume (V) and the degree of enophthalmos (E) was E= 0.851 V-0.197 (Pearson r=0.969, P＜0.01, the 95% confidence intervals of 0.732-0.970). The standardized equation between the enlarged volume of floor together with that of medial wall and the degree of enophthalmos was E= 0.690 VF+0.413 VM-0. 086 (setpwise, entry =0.5, removal= 0. 11,P＜0.01 ); Comparing the standardized coefficients of independent variables, the outcome was bF (0.690) ＞ bM(0.413). Conclusion Significant linear correlation between the increment of the orbital volume and the degree of enophthalmos is found that 1.0 ml enlargeement in bony volume causes approximately 0.9 mm of enophthalmos; enlarged volume of floor has more powerful influence on the degree of enophthalmos than enlarged volume of medial wall, the former is 1.56 times of the latter.

ObjectiveTo evaluate radio-frequency hemostasis in hepatectomy.MethodsFrom January 2009 to February 2011,the clinical data of 60 patients undergoing curative liver resection were divided into two groups using radio-frequency hemostasis (RFH) and clamp crushing method (CCM) respectively,RFH group (30 cases) and CCM group (30 cases).There was no difference between the 2 groups regarding the age,sex.hepatic function and tumor size.Data regarding the intra-operative and postoperative courses of the patients were analyzed.ResultsNo damage of hepatic vein occured in RFH group.Hepatic veins rupture occurred in 5 cases and massive bleeding occurred in 3 cases in CCM group.lntra-operative blood loss was significantly less in FRH group [ (219 ±62) ml] than in CCM group [ (416 ±96) ml ] (P ＜ 0.05 ).The postoperative drainage volume in RFH group was significantly less than that in CCM group on the third postoperative day.The serum ALT and T-BIL in RFH group was significantly lower than that in CCM group on postoperative day 1 and day 7 ( separately t =5.987,16.803,22.264,8.386,8.255,all P ＜0.05 ).Postoperative hepatic function in RFH group was significantly better than that in CCM group.ConclusionsThe use of radio-frequency hemostasis in hepatectomy is less traumatic,of less bleeding,faster recovery than clamp crashing method.